Date published: 2026-10-24

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PRMT1 Antibody (B-2) - Western Blotting - Image 129877
PRMT1 抗体 (B-2): sc-166963. COLO 320DM (A), HeLa (B), IMR-32 (C) 和 WI-38 (D) 全细胞裂解液中 PRMT1 表达的 Western 印迹分析.
PRMT1 抗体 (B-2): sc-166963. COLO 320DM (A), HeLa (B), IMR-32 (C) 和 WI-38 (D) 全细胞裂解液中 PRMT1 表达的 Western 印迹分析.

PRMT1 抗体 (B-2): sc-166963

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寫評論提問
说明书
  • PRMT1 抗体 B-2 是小鼠单克隆 IgG1 κ，PRMT1抗体, 在21篇文献中引用，规格为200 µg/ml
  • 针对氨基酸166-300的图谱，在PRMT1的内部区域，human起源
  • PRMT1 抗体 (B-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PRMT1
  • 抗PRMT1抗体（B-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是PRMT1 Antibody (B-2) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与PRMT1 Antibody (B-2) 打包提供（请参阅下面的订购信息）。
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関連項目

额外PRMT抗体，包括PRMT1, PRMT2, PRMT5, PRMT6和PRMT7

PRMT1抗体（B-2）是一种IgG1κ小鼠单克隆PRMT1抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的PRMT1蛋白。PRMT1抗体（B-2）可作为非偶联抗PRMT1抗体形式，以及多种偶联形式的抗PRMT1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。一类称为1型蛋白质精氨酸N-甲基转移酶（PRMTs）酶的蛋白质参与RNA结合蛋白的翻译后修饰，但在底物特异性、寡聚化特性和亚细胞定位方面有所不同。PRMT1是哺乳动物细胞中的主要形式，位于细胞核中。在羧基末端，白细胞介素增强因子结合因子3（ILF3）与PRMT1结合，从而调节PRMT1的活性。PRMT基因的替代mRNA剪接导致三种不同亚型的PRMT1，其氨基末端区域不同，都具有酶活性。PRMT8，也称为HRMT1L3或HRMT1L4（异质核核糖核蛋白甲基转移酶样蛋白4），是1型PRMT家族的一个独特成员，具有组织特异性表达和质膜定位。PRMT8在脑中特异性表达，在神经元分化中可能具有精氨酸甲基转移酶的作用。它与PRMT1最密切相关，可能是通过基因复制产生的。PRMT8可以与PRMT1异二聚化，具有相似的底物偏好。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PRMT1 抗体 (B-2) 参考文献:

  1. 骨骼肌特异性 Prmt1 基因缺失会通过 PRMT6-FOXO3 轴的失调导致肌肉萎缩。  |  Choi, S., et al. 2019. Autophagy. 15: 1069-1081. PMID: 30653406
  2. PRMT1对EZH2的甲基化调节其稳定性并促进乳腺癌转移。  |  Li, Z., et al. 2020. Cell Death Differ. 27: 3226-3242. PMID: 32895488
  3. PRMT1介导的H4R3me2a通过增强EGFR信号传导，招募SMARCA4促进结直肠癌的进展。  |  Yao, B., et al. 2021. Genome Med. 13: 58. PMID: 33853662
  4. PRMT1对PHGDH精氨酸的甲基化促进丝氨酸的合成，是肝癌治疗的一个薄弱环节。  |  Wang, K., et al. 2023. Nat Commun. 14: 1011. PMID: 36823188
  5. 靶向PRMT1介导的SRSF1甲基化抑制致癌外显子插入事件和乳腺癌的发生。  |  Li, WJ., et al. 2023. Cell Rep. 42: 113385. PMID: 37938975
  6. PRMT1抑制激活干扰素通路，增强黑色素瘤的抗肿瘤免疫并提高检查点阻断功效。  |  Tao, H., et al. 2024. Cancer Res. 84: 419-433. PMID: 37991725
  7. PRMT1通过甲基化PHGDH维持从头脂肪酸合成，从而驱动三阴性乳腺癌的耐药性。  |  Yamamoto, T., et al. 2024. Cancer Res. 84: 1065-1083. PMID: 38383964
  8. Git1-PGK1相互作用通过调节Keap1/Nrf2信号传导实现脊髓缺血再灌注损伤的自我保护。  |  Liu, H., et al. 2024. Cell Death Dis. 15: 170. PMID: 38402202
  9. PRMT1促进胰腺癌的发展和化疗耐药性。  |  Ku, B., et al. 2024. Cell Rep Med. 5: 101461. PMID: 38460517
  10. CRISPR筛选通过双层调节机制确定PRMT1为关键促铁死亡基因。  |  Zhang, X., et al. 2024. Cell Rep. 43: 114662. PMID: 39178116

订购信息

产品名称产品编号规格价格数量收藏夹

PRMT1 抗体 (B-2)

sc-166963
200 µg/ml
CNY2422.00

PRMT1 (B-2): m-IgG Fc BP-HRP 套装

sc-527201
200 µg Ab; 10 µg BP
CNY2715.00

PRMT1 (B-2): m-IgG1 BP-HRP 套装

sc-532574
200 µg Ab; 20 µg BP
CNY2715.00

PRMT1 抗体 (B-2) AC

sc-166963 AC
500 µg/ml, 25% agarose
CNY3189.00

PRMT1 抗体 (B-2) HRP

sc-166963 HRP
200 µg/ml
CNY2422.00

PRMT1 抗体 (B-2) FITC

sc-166963 FITC
200 µg/ml
CNY2527.00

PRMT1 抗体 (B-2) PE

sc-166963 PE
200 µg/ml
CNY2625.00

PRMT1 抗体 (B-2) Alexa Fluor® 488

sc-166963 AF488
200 µg/ml
CNY2738.00

PRMT1 抗体 (B-2) Alexa Fluor® 546

sc-166963 AF546
200 µg/ml
CNY2738.00

PRMT1 抗体 (B-2) Alexa Fluor® 594

sc-166963 AF594
200 µg/ml
CNY2738.00

PRMT1 抗体 (B-2) Alexa Fluor® 647

sc-166963 AF647
200 µg/ml
CNY2738.00

PRMT1 抗体 (B-2) Alexa Fluor® 680

sc-166963 AF680
200 µg/ml
CNY2738.00

PRMT1 抗体 (B-2) Alexa Fluor® 790

sc-166963 AF790
200 µg/ml
CNY2738.00

I want to use this antibody to make a experiment of immunoprecipitation in the mouse preadipocytes，can you ensure you product can satisfy my requirement ? Or maybe I need to apply for a trial product.

Asked by: Anonymous
Thanks for your questions. Anti-PRMT1 Antibody (B-2) is recommended for detection of PRMT1 of mouse origin by IP. 感谢您的问题。Anti-PRMT1 抗体 (B-2)是推荐免疫沉淀方法检测小鼠来源样本的，该产品不质保免疫共沉淀。
Answered by: Technical Support 9
Date published: 2020-06-12

I am using PRMT1 (B-2): sc-166963 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by roberto_giambruno from The sc-166963 antibody does not workI prepared 10µg of whole cell lysates of HuH7 or PLC cells and run them on a 10% SDS-PAGE gel. Proteins were transferred from the gel into a nitrocellulose membrane and after blocking, membranes were incubated with the respective antibody rocking them in a shaker at 4 degree over night. The membranes were then washed and incubated with the appropriate secondary antibody and developed with the LI-COR Odyssey imager. The antibody against PRMT1 did not properly work and it produced a not specific staining.
Date published: 2024-01-23
Rated 1 out of 5 by HarryH from This Ab did not work.I recently purchased this Ab and found it did not work for WB. There was no any band detected in my several cell lines and I did detected the PRMT1 bands using Cell Signaling PRMT1 Ab (Cat# 2449S, This Ab detected two bands in my cell lines. Therefore I tried to use Santa Cruz mAb). I asked for replacing it and found the replaced one did not work either.
Date published: 2022-09-02
Rated 5 out of 5 by TINGEr from high-activeStrong bands were observed in human tissues by immunoblotting, and it was very effective for WB.
Date published: 2017-10-26
Rated 5 out of 5 by quyue from good performanceI usually use this PRMT1 anitbody .This PRMT1 antibody worked very well for WB, it be diluted 1:1000,we got clear and strong signal at 42kda
Date published: 2017-09-02
Rated 5 out of 5 by panlilong from Excellent antibodya free sample, but a good result !! I will continue to support SCBT
Date published: 2017-08-15
Rated 4 out of 5 by bode from this antibody also can be used mouse ES cellsthis antibody also can be used mouse ES cells and can be detected about 35KDa.
Date published: 2017-06-27
Rated 5 out of 5 by Big king from good result.This was used for IP detection in AGS, when diluted 1:1000 is a good signal.
Date published: 2017-06-21
Rated 5 out of 5 by zhiqing from specific signalI am glad to buy this antibody. I used it to do western blot .It have signal in Hela cell line.
Date published: 2017-05-31
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PRMT1 抗体 (B-2) is rated 4.3 out of 5 by 12.
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