Date published: 2025-9-6

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PRMT1 抗体 (PRMT1-171): sc-59648

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说明书
  • PRMT1 抗体 PRMT1-171 是小鼠单克隆 IgG1, 在11篇文献中引用,提供 100 µg/ml
  • 针对全长PRMT1,human起源
  • 推荐用于 mouse, rat, human, bovine 和 canine 来源的PRMT1 WB, IP 和 ELISA检测; 不会与 PRMT2, 3, 4, or 5产生交联反应
  • 关于如何获取PRMT1 (PRMT1-171): sc-59648免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是PRMT1 Antibody (PRMT1-171) for WB applications. 的首选辅助检测试剂,这些试剂现与PRMT1 Antibody (PRMT1-171) 打包提供(请参阅下面的订购信息)。

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関連項目

PRMT1抗体(PRMT1-171)是一种IgG1小鼠单克隆PRMT1抗体,可通过WB、IP和ELISA检测小鼠、大鼠和人源的PRMT1。PRMT1抗体(PRMT1-171)以非偶联抗PRMT1抗体形式提供。一类称为1型蛋白质精氨酸N-甲基转移酶(PRMT)酶的蛋白质参与RNA结合蛋白的翻译后修饰,但在底物特异性、寡聚化特性和亚细胞定位方面有所不同。PRMT1是哺乳动物细胞中主要的形式,位于细胞核中,而PRMT3存在于细胞质中。在羧基末端,白细胞介素增强子结合因子3(ILF3)与PRMT1结合,从而调节PRMT1的活性。PRMT基因的mRNA选择性剪接导致三种不同氨基末端区域的PRMT1异构体。PRMT1的所有三种剪接变异体都具有酶活性。PRMT3在其氨基末端识别并结合具有锌指结构域的RNA相关底物。该结构域的锌配体形式是酶识别RNA相关底物所必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PRMT1 抗体 (PRMT1-171) 参考文献:

  1. PRMT1 是哺乳动物细胞中最主要的 I 型蛋白质精氨酸甲基转移酶。  |  Tang, J., et al. 2000. J Biol Chem. 275: 7723-30. PMID: 10713084
  2. 蛋白精氨酸甲基转移酶 I 是细胞中最主要的蛋白精氨酸甲基转移酶,它与白细胞介素增强子结合因子 3 相互作用并受其调节。  |  Tang, J., et al. 2000. J Biol Chem. 275: 19866-76. PMID: 10749851
  3. PRMT3 是精氨酸 N-甲基转移酶家族中的一个独特成员。通过锌指结构域实现底物特异性。  |  Frankel, A. and Clarke, S. 2000. J Biol Chem. 275: 32974-82. PMID: 10931850
  4. 人类精氨酸甲基转移酶 1 基因的基因组结构, 物理图谱和表达分析。  |  Scorilas, A., et al. 2000. Biochem Biophys Res Commun. 278: 349-59. PMID: 11097842
  5. 主要蛋白质精氨酸甲基转移酶 PRMT1 的结构及其与底物肽的结合分析。  |  Zhang, X. and Cheng, X. 2003. Structure. 11: 509-20. PMID: 12737817
  6. PRMT1, p300 和 CARM1 在 p53 激活转录过程中的有序合作功能。  |  An, W., et al. 2004. Cell. 117: 735-48. PMID: 15186775
  7. DNA 损伤检查点控制需要 PRMT1 对 MRE11 进行精氨酸甲基化。  |  Boisvert, FM., et al. 2005. Genes Dev. 19: 671-6. PMID: 15741314
  8. CDK5-PRMT1-WDR24 信号级联促进了 mTORC1 信号转导和肿瘤生长。  |  Yin, S., et al. 2023. Cell Rep. 42: 112316. PMID: 36995937
  9. PRMT1介导的cGAS甲基化抑制抗肿瘤免疫。  |  Liu, J., et al. 2023. Nat Commun. 14: 2806. PMID: 37193698
  10. PRMT1与SAMTOR协同作用,通过NPRL2的精氨酸甲基化来调节mTORC1的蛋氨酸感应。  |  Jiang, C., et al. 2023. Cell Metab. 35: 2183-2199.e7. PMID: 38006878
  11. FBXO7泛素化PRMT1以抑制肝细胞癌中的丝氨酸合成和肿瘤生长。  |  Luo, L., et al. 2024. Nat Commun. 15: 4790. PMID: 38839752
  12. PRMT 3 是一种 I 型精氨酸 N-甲基转移酶,在寡聚化, 亚细胞定位, 底物特异性和调控方面与 PRMT1 不同。  |  Tang, J., et al. 1998. J Biol Chem. 273: 16935-45. PMID: 9642256

订购信息

产品名称产品编号规格价格数量收藏夹

PRMT1 抗体 (PRMT1-171)

sc-59648
100 µg/ml
RMB2377.00

PRMT1 (PRMT1-171): m-IgG Fc BP-HRP 套装

sc-539137
100 µg Ab; 10 µg BP
RMB2662.00

PRMT1 (PRMT1-171): m-IgG1 BP-HRP 套装

sc-541285
100 µg Ab; 20 µg BP
RMB2662.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with PRMT1 (PRMT1-171): sc-59648 antibody?

Asked by: cjMara
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Great antibody. It works well.The antibody works very well and recognizes only one band at the expected molecular weight of PRMT1.
Date published: 2024-06-13
Rated 1 out of 5 by from Ab did not workThis Ab did not work and I have to switch to other company's product.
Date published: 2022-09-19
Rated 5 out of 5 by from Excellent in Western BlottingDilution: 1:1000 Wester blotting on HeLA cell lysate Results: very specific and clear band at the expected molecular weight
Date published: 2019-12-11
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of PRMT1 expression in PC-3 whole cell lysate.
Date published: 2017-02-01
Rated 5 out of 5 by from Published for IHC and WB in human Hodgkin'sPublished for IHC and WB in human Hodgkin's lymphoma (HL) cell lines(L591,KMH2,L428,L540,L1236), GC B cells, Hodgkin Reed-Sternberg (HRS) cells. -SCBT Publication Review
Date published: 2015-04-07
Rated 5 out of 5 by from Strong signal strength in WB in PCStrong signal strength in WB in PC-3 whole cell lysate. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Strong band in WB in human keratinocytesStrong band in WB in human keratinocytes. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Good specificity in WB in mouse transfectedGood specificity in WB in mouse transfected 293T and PC-1 whole cell lysates. -SCBT QC
Date published: 2015-01-08
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PRMT1 抗体 (PRMT1-171) is rated 4.6 out of 5 by 9.
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