Date published: 2025-9-8

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PREP-1 抗体 (B-2): sc-25282

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说明书
  • PREP-1 抗体 B-2 是小鼠单克隆 IgG1 κ,PREP-1抗体, 在10篇文献中引用,规格为200 µg/ml
  • 抗-PREP-1 抗体 (B-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PREP-1
  • PREP-1抗体(B-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-25282 X, 200 µg/0.1 ml)
  • 关于如何获取PREP-1 (B-2): sc-25282免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 PREP-1 抗体 (B-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PREP-1 抗体 (B-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PREP-1抗体(B-2)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的PREP-1蛋白。PREP-1 (B-2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。PREP-1在转录调控中起着至关重要的作用,特别是在前B细胞急性白血病中,PREP-1通常与染色体重排有关,例如t(1;19)(q23;p13.3),导致E2A和Pbx1之间形成融合基因。这种融合产生的蛋白质将E2A的转激活结构域与Pbx1的同源框结构域结合在一起,这对于调节细胞增殖和分化基因至关重要。此外,PREP-1与Pbx蛋白相互作用,形成稳定的复合物,从而增强尿激酶基因的转录,影响细胞迁移和侵袭等过程。PREP-1能够调节转录活性,使其成为正常细胞功能和病理条件下的重要因素,凸显了PREP-1在癌症研究和治疗开发中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PREP-1 抗体 (B-2) 参考文献:

  1. PBX2和PBX3,与人类原癌基因PBX1具有广泛同源性的新同源基因。  |  Monica, K., et al. 1991. Mol Cell Biol. 11: 6149-57. PMID: 1682799
  2. 染色体易位t(1;19)导致合成了一个同源染色体融合的mRNA,该mRNA编码一个潜在的嵌合转录因子。  |  Nourse, J., et al. 1990. Cell. 60: 535-45. PMID: 1967982
  3. 一个新的同源染色体基因为前B型 ALL的t(1;19)易位蛋白的DNA结合域做出了贡献。  |  Kamps, MP., et al. 1990. Cell. 60: 547-55. PMID: 1967983
  4. Pknox1/Prep1调节骨骼肌线粒体氧化磷酸化成分。  |  Kanzleiter, T., et al. 2014. Mol Cell Biol. 34: 290-8. PMID: 24216763
  5. Prep1 缺乏会改善白色脂肪组织的代谢反应。  |  Liotti, A., et al. 2018. Biochim Biophys Acta Mol Cell Biol Lipids. 1863: 515-525. PMID: 29474930
  6. Prep1,一种参与葡萄糖和脂质代谢的同源基因转录因子。  |  Oriente, F., et al. 2018. Front Endocrinol (Lausanne). 9: 346. PMID: 30002646
  7. PREP1 homeodomain 转录因子的遗传学和分子功能。  |  Purushothaman, D. and Blasi, F. 2018. Int J Dev Biol. 62: 819-825. PMID: 30604851
  8. Prep1 通过 PGC-1&alpha-mediated 机制调节血管生成。  |  Cimmino, I., et al. 2019. FASEB J. 33: 13893-13904. PMID: 31618597
  9. 在携带 t(1;19) 易位的人类白血病中,与 E2A 融合可将 Pbx1 同源域蛋白转化为组成型转录激活因子。  |  Lu, Q., et al. 1994. Mol Cell Biol. 14: 3938-48. PMID: 7910944
  10. E2a-Pbx1 嵌合肿瘤蛋白的转化特性:与 E2a 融合是必要的,但 Pbx1 同源域则是可有可无的。  |  Monica, K., et al. 1994. Mol Cell Biol. 14: 8304-14. PMID: 7969166
  11. 与 E2A 融合会改变 t(1;19)白血病中同源结构域蛋白 PBX1 的转录特性。  |  LeBrun, DP. and Cleary, ML. 1994. Oncogene. 9: 1641-7. PMID: 8183558
  12. 参与调节尿激酶和其他 AP-1 控制的启动子的新型因子 UEF3 的纯化和特征描述。  |  Berthelsen, J., et al. 1996. J Biol Chem. 271: 3822-30. PMID: 8632000

订购信息

产品名称产品编号规格价格数量收藏夹

PREP-1 抗体 (B-2)

sc-25282
200 µg/ml
RMB2377.00

PREP-1 (B-2): m-IgG Fc BP-HRP 套装

sc-528393
200 µg Ab; 10 µg BP
RMB2662.00

PREP-1 (B-2): m-IgGκ BP-HRP 套装

sc-520763
200 µg Ab, 40 µg BP
RMB2662.00

PREP-1 (B-2): m-IgG1 BP-HRP 套装

sc-542912
200 µg Ab; 20 µg BP
RMB2662.00

PREP-1 抗体 (B-2) X

sc-25282 X
200 µg/0.1 ml
RMB2377.00

PREP-1 抗体 (B-2) AC

sc-25282 AC
500 µg/ml, 25% agarose
RMB3129.00

PREP-1 抗体 (B-2) HRP

sc-25282 HRP
200 µg/ml
RMB2377.00

PREP-1 抗体 (B-2) FITC

sc-25282 FITC
200 µg/ml
RMB2482.00

PREP-1 抗体 (B-2) PE

sc-25282 PE
200 µg/ml
RMB2580.00

PREP-1 抗体 (B-2) Alexa Fluor® 488

sc-25282 AF488
200 µg/ml
RMB2685.00

PREP-1 抗体 (B-2) Alexa Fluor® 546

sc-25282 AF546
200 µg/ml
RMB2685.00

PREP-1 抗体 (B-2) Alexa Fluor® 594

sc-25282 AF594
200 µg/ml
RMB2685.00

PREP-1 抗体 (B-2) Alexa Fluor® 647

sc-25282 AF647
200 µg/ml
RMB2685.00

PREP-1 抗体 (B-2) Alexa Fluor® 680

sc-25282 AF680
200 µg/ml
RMB2685.00

PREP-1 抗体 (B-2) Alexa Fluor® 790

sc-25282 AF790
200 µg/ml
RMB2685.00

Dear Sir, I have 2 quesions: 1. Does this antibody work well for chip(PREP-1 Antibody (B-2) sc-25282)? 2. Does this antibody still work for chip and immunohistochemical experiment after left at room temperature overnight? Thank you!

Asked by: zhidong
Thank you for your question. PREP-1 Antibody (B-2): sc-25282 is recommended for detection of PREP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, so you can perform immunohistochemistry with this antibody using the standard protocol provided on our website. A TransCruz reagent for Gel Supershift and ChIP applications with higher concentration and an optimized buffer is also available for this antibody: sc-25282 X. You can also take a look at publication PMID 30337366, in which the antibody has been used for ChIP.
Answered by: Tech Support Europe
Date published: 2019-06-06

When should the TransCruz reagent sc-25282 X be used instead of the standard form sc-25282?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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PREP-1 抗体 (B-2) is rated 5.0 out of 5 by 2.
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