Date published: 2025-9-8
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PPARγ Antibody (B-5) - Western Blotting - Image 134070
PPARγ (B-5): sc-271392. 非转染: sc-117752 (A) 和人 PPARγ 转染: sc-110516 (B) 293T 全细胞裂解液中 PPARγ 表达的 Western 印迹分析.
PPARγ (B-5): sc-271392. 非转染: sc-117752 (A) 和人 PPARγ 转染: sc-110516 (B) 293T 全细胞裂解液中 PPARγ 表达的 Western 印迹分析.

PPARγ 抗体 (B-5): sc-271392

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寫評論提問

说明书
  • PPARγ抗体(B-5)是小鼠单克隆IgG1 λ, 在64篇文献中引用，规格为200 µg/ml
  • 免疫human物种的PPARγ的氨基酸8-106
  • 抗-PPAR gamma 抗体 (B-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 PPARγ1 and PPARγ2
  • 抗PPAR gamma抗体（B-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-271392 X, 200 µg/0.1 ml)
  • 关于如何获取PPARγ (B-5): sc-271392的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP（小鼠 IgGλ 结合蛋白-HRP）是PPARγ Antibody (B-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与PPARγ Antibody (B-5) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGλ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加PPAR抗体，包括PPAR α, PPAR β, PPAR γ和PPAR γ 2

PPARγ抗体（B-5）是一种IgG1 λ小鼠单克隆PPARγ抗体（也称为PPARγ抗体或PPAR gamma抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的PPARγ1和PPARγ2。PPARγ抗体（B-5）既可以是非偶联的抗PPARγ抗体形式，也可以是多种偶联形式的抗PPARγ抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。过氧化物酶体增殖物激活受体（PPARs）是转录因子核激素受体亚家族的成员。PPARs与视黄醇X受体（RXRs）形成异二聚体。这些异二聚体调节参与胰岛素作用、脂肪细胞分化、脂质代谢和炎症的基因的转录。PPARγ与多种疾病有关，包括肥胖、糖尿病、动脉粥样硬化和癌症。PPARγ激活剂包括前列腺素类、脂肪酸、噻唑烷二酮和N-(2-苯甲酰基苯基)酪氨酸类似物。作为脂肪细胞分化和脂肪特异性基因表达的关键组件，PPARγ可调节巨噬细胞功能，如促炎活动，并刺激氧化低密度脂蛋白（ox-LDL）的摄取。据报道，PPARγ2基因的Pro12Ala多态性可在体外降低反式激活活性。这种替代可能影响对ox-LDL的免疫反应，并与2型糖尿病相关。此外，PPARγ2基因的Pro12Ala变异可能与2型糖尿病中的腹部肥胖相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

PPARγ 抗体 (B-5)

sc-271392
200 µg/ml
RMB2377.00

PPARγ (B-5): m-IgGλ BP-HRP 套装

sc-521929
200 µg Ab, 40 µg BP
RMB2662.00

PPARγ 抗体 (B-5) X

sc-271392 X
200 µg/0.1 ml
RMB2377.00

PPARγ 抗体 (B-5) AC

sc-271392 AC
500 µg/ml, 25% agarose
RMB3129.00

PPARγ 抗体 (B-5) HRP

sc-271392 HRP
200 µg/ml
RMB2377.00

PPARγ 抗体 (B-5) FITC

sc-271392 FITC
200 µg/ml
RMB2482.00

PPARγ 抗体 (B-5) PE

sc-271392 PE
200 µg/ml
RMB2580.00

PPARγ 抗体 (B-5) Alexa Fluor® 488

sc-271392 AF488
200 µg/ml
RMB2685.00

PPARγ 抗体 (B-5) Alexa Fluor® 546

sc-271392 AF546
200 µg/ml
RMB2685.00

PPARγ 抗体 (B-5) Alexa Fluor® 594

sc-271392 AF594
200 µg/ml
RMB2685.00

PPARγ 抗体 (B-5) Alexa Fluor® 647

sc-271392 AF647
200 µg/ml
RMB2685.00

PPARγ 抗体 (B-5) Alexa Fluor® 680

sc-271392 AF680
200 µg/ml
RMB2685.00

PPARγ 抗体 (B-5) Alexa Fluor® 790

sc-271392 AF790
200 µg/ml
RMB2685.00

Is this antibody available as "unconjugated?"

Asked by: 23Cae
Thank you for your question. Yes, the standard format for this and all antibodies is unconjugated. Please reference PPARγ Antibody (B-5): sc-271392. This antibody is also available conjugated. Please refer to the product block above for which conjugated forms are available.
Answered by: Technical Support
Date published: 2019-01-26

do you have peptide for PPARr B5 sc-271392?

Asked by: Haimei
Thank you for your question. This mouse monoclonal antibody is not raised against a peptide, it is raised against a recombinant protein corresponding to amino acids 8-106 of PPARγ of human origin. No blocking peptide is available.
Answered by: Technical Support
Date published: 2019-01-26

I am Vishva from Ohio University. I would like to try two of your antibodies (SC-271392 and SC-6247). Would it be possible for you to send me the free sample for the trial. If it works, I can then buy these products.

Asked by: Vishva
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

Does PPARγ Antibody (B-5): sc-271392 react with avian samples?

Asked by: sweetums
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-20

When should the TransCruz reagent sc-271392 X be used instead of the standard form sc-271392? What is the difference between the two forms?

Asked by: sweetums
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration. The TransCruz reagent is provided as 10x more concentrated than the regular form: sc-271392 X, 200 µg/0.1 ml.
Answered by: Technical Support 1
Date published: 2017-01-16
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Rated 5 out of 5 by from 功能很出色已经购买过santacruz的一抗，santacruz的抗体效果还是不错的，还有小包装和试用装。很赞！
Date published: 2020-05-20
Rated 1 out of 5 by from didn't workit didn't work but we've only tried once. dillution 1/500
Date published: 2019-04-01
Rated 5 out of 5 by from PPARgamma in rat liversReceived this antibody as a sample. Was able to detect the protein in rat livers. However, the antibody needs to be incubated overnight in blocking buffer.
Date published: 2018-07-11
Rated 5 out of 5 by from The antibody works well.I bought the antibody a month ago, and I am happy that It did work very well.
Date published: 2017-11-26
Rated 5 out of 5 by from Strong westernVery nice western data; strong signal with low background
Date published: 2017-01-26
Rated 5 out of 5 by from No Non-specific BandsVery clean blots seen in WB, single strong target band with no additional signal.
Date published: 2017-01-17
Rated 4 out of 5 by from Good specificity in WB at 1:200 dilutionGood specificity in WB at 1:200 dilution in human prostate cancer cell lines. -SCBT Publication Review
Date published: 2015-03-19
Rated 5 out of 5 by from Good antibody for IHC at 1:200 dilution inGood antibody for IHC at 1:200 dilution in human aortic wall segments. -SCBT Publication Review
Date published: 2015-03-13
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PPARγ 抗体 (B-5) is rated 4.4 out of 5 by 11.
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