Date published: 2025-9-6
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PP1α Antibody (G-4) - Western Blotting - Image 352015
PP1α 抗体 (G-4): sc-271762. BT-20 (A), PC-12 (B), C32 (C), SK-BR-3 (D), Jurkat (E) 和 KNRK (F) 全细胞裂解液中 PP1α 表达的 Western 印迹分析.
PP1α 抗体 (G-4): sc-271762. BT-20 (A), PC-12 (B), C32 (C), SK-BR-3 (D), Jurkat (E) 和 KNRK (F) 全细胞裂解液中 PP1α 表达的 Western 印迹分析.

PP1α 抗体 (G-4): sc-271762

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说明书
  • PP1 alpha 抗体 G-4 是小鼠单克隆 IgG1 κ，PP1α抗体, 在26篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PP1α的C-terminus的氨基酸297-326之间
  • PP1 alpha 抗体 (G-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 PP1α; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 抗PP1 alpha抗体（G-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PP1α (G-4): sc-271762的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是PP1α Antibody (G-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PP1α Antibody (G-4) 打包提供（请参阅下面的订购信息）。

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描述
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说明书与实验方案
研究信息

関連項目

额外PP1抗体，包括PP1 β

PP1α抗体（G-4）是一种IgG1 κ小鼠单克隆PP1α抗体（也被指定为PPP1CA抗体、PPP1A抗体，或丝氨酸/苏氨酸蛋白磷酸酶PP1-α催化亚基抗体），它能够通过WB、IP、IF和ELISA检测到小鼠、大鼠和人类来源的PP1α蛋白。PP1α抗体（G-4）既有非偶联型抗PP1α抗体形式，也有多种偶联型抗PP1α抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在真核生物中，丝氨酸和苏氨酸残基上蛋白质的磷酸化和去磷酸化是调节细胞广泛功能的重要手段，包括细胞分裂、稳态和凋亡。密切参与这一过程的蛋白质是蛋白磷酸酶。通常，蛋白磷酸酶（PP）全酶是由调节亚基、可变亚基和催化亚基组成的三聚体复合物。已鉴定出四个主要的蛋白磷酸酶催化亚基家族，分别被命名为PP1、PP2A、PP2B（钙调磷酸酶）和PP2C。另外一个蛋白磷酸酶催化亚基PPX（也被称为PP4）是一个新型PP家族的假定成员。PP1家族由亚家族成员PP1α、PP1β和PP1γ组成，它们都是MgATP依赖性酶。PP1的失活是通过与抑制性蛋白NIPP-1（PP1的核抑制剂）结合来维持的。NIPP-1被cAMP-PK或酪蛋白激酶II磷酸化后，会释放出活性的PP1。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PP1α 抗体 (G-4) 参考文献:

  1. 蛋白磷酸酶-1 的核抑制剂 NIPP-1 的分子克隆揭示了其与参与 RNA 处理的多肽的同源性。  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
  2. 丝氨酸/苏氨酸蛋白磷酸酶。  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
  3. 新型蛋白磷酸酶对丝氨酸和苏氨酸残基去磷酸化的重要作用  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
  4. 平滑肌肌球蛋白结合磷酸酶的调节亚基。  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
  5. 蛋白磷酸酶 2A 72 kDa 调节亚基的结构和表达。替代剪接产生不同大小形式的证据。  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
  6. 蛋白丝氨酸/苏氨酸磷酸酶：结构, 调节和在细胞生长中的功能。  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

订购信息

产品名称产品编号规格价格数量收藏夹

PP1α 抗体 (G-4)

sc-271762
200 µg/ml
RMB2377.00

PP1α (G-4): m-IgGκ BP-HRP 套装

sc-551993
200 µg Ab; 40 µg BP
RMB2662.00

PP1α (G-4): m-IgG1 BP-HRP 套装

sc-552056
200 µg Ab; 20 µg BP
RMB2662.00

PP1α 抗体 (G-4) AC

sc-271762 AC
500 µg/ml, 25% agarose
RMB3129.00

PP1α 抗体 (G-4) HRP

sc-271762 HRP
200 µg/ml
RMB2377.00

PP1α 抗体 (G-4) FITC

sc-271762 FITC
200 µg/ml
RMB2482.00

PP1α 抗体 (G-4) PE

sc-271762 PE
200 µg/ml
RMB2580.00

PP1α 抗体 (G-4) Alexa Fluor® 488

sc-271762 AF488
200 µg/ml
RMB2685.00

PP1α 抗体 (G-4) Alexa Fluor® 546

sc-271762 AF546
200 µg/ml
RMB2685.00

PP1α 抗体 (G-4) Alexa Fluor® 594

sc-271762 AF594
200 µg/ml
RMB2685.00

PP1α 抗体 (G-4) Alexa Fluor® 647

sc-271762 AF647
200 µg/ml
RMB2685.00

PP1α 抗体 (G-4) Alexa Fluor® 680

sc-271762 AF680
200 µg/ml
RMB2685.00

PP1α 抗体 (G-4) Alexa Fluor® 790

sc-271762 AF790
200 µg/ml
RMB2685.00

PP1α (G-4) 中和勝肽

sc-271762 P
100 µg/0.5 ml
RMB511.00

Was this product validated for isotope specificity, i.e. does it detect only PP1alpha and not PP1beta and PP1gamma?

Asked by: cstech
PP1α Antibody (G-4) is specific for an epitope mapping between amino acids 297-326 at the C-terminus of PP1α of human origin. It does not detect PP1β or PP1γ. For an overview of all antibodies that detect PP1 or one of its isotypes, just follow this link: https://www.scbt.com/scbt/browse/pp1-Antibodies/_/N-nb1lg9 Please contact technical service if you have further questions.
Answered by: Technical Support Europe
Date published: 2019-10-11

What application is the blocking peptide sc-271762 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Weak signal but specific for WB, IPCould detect but a little weak signal but specific for WB(x200~500, 1hr~o/n), IP(1 µg) in HeLa S3 cells
Date published: 2022-08-07
Rated 5 out of 5 by from Great antibodyThis antibody could help you get good WB images, however, this antibody is not good enough for IF exp.
Date published: 2020-08-13
Rated 5 out of 5 by from Good for WB, but not ChIPI tried it for both WB and ChIP on human cells. I worked for WB but not ChIP.
Date published: 2020-07-12
Rated 2 out of 5 by from A weak antibodyCannot detect endogenous PP1a in HEK293 and A549 cells. A very weak band was found in RAW264.7 although the size was correct.
Date published: 2017-11-30
Rated 5 out of 5 by from strong band at 37kdaThe antibody used for analysis PP1a protein expression in metastatic and non metastatic malignant melanoma.We suggest use 1:600 dilution by WB,not too much loading the sample (controled around 50ug),It gave us strong signal at 37kda.
Date published: 2017-09-09
Rated 5 out of 5 by from Widely ReactiveGreat data with mouse, rat and human cell lines in Western Blot, good choice when working with non-model species such as canine, bovine and porcine.
Date published: 2017-01-27
Rated 4 out of 5 by from Publishable data in IP using human t cellsPublishable data in IP using human t cells. -SCBT Publication Review
Date published: 2015-02-26
Rated 4 out of 5 by from Robust band seen in WB using NIH/3T3 wholeRobust band seen in WB using NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-01-26
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PP1α 抗体 (G-4) is rated 4.2 out of 5 by 10.
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