Date published: 2025-9-6
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PP1 Antibody (E-9) - Western Blotting - Image 376868
PP1 抗体 (E-9) HRP: sc-7482 HRP. 在 Jurkat (A), KNRK (B), PC-12 (C), A-431 (D), MDA-MB-231 (E) 和 MCF7 (F) 全细胞裂解液中 PP1 表达的直接 Western 印迹分析.
PP1 抗体 (E-9) HRP: sc-7482 HRP. 在 Jurkat (A), KNRK (B), PC-12 (C), A-431 (D), MDA-MB-231 (E) 和 MCF7 (F) 全细胞裂解液中 PP1 表达的直接 Western 印迹分析.

PP1 抗体 (E-9): sc-7482

4.7(24)
寫評論提問

说明书
  • PP1 抗体 E-9 是小鼠单克隆 IgG2b κ，PP1抗体, 在206篇文献中引用，规格为200 µg/ml
  • 针对全长磷酸酶PP1α，human来源
  • PP1 抗体 (E-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PP1 family catalytic subunits; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 抗PP1抗体（E-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PP1 (E-9): sc-7482的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 PP1 抗体 (E-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PP1 抗体 (E-9) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 PP1 抗体包括 PP1 α 和 PP1 β

PP1抗体（E-9）是一种小鼠单克隆IgG2b kappa轻链抗体，用于识别全长人蛋白磷酸酶1催化亚基α（PP1α），也称为PPP1CA或蛋白磷酸酶1α。PP1α单克隆抗体（E-9）通过靶蛋白上丝氨酸和苏氨酸残基的可逆去磷酸化，参与细胞过程的调节，包括细胞分裂、稳态和凋亡。 PP1α对磷酸化状态的这种调节对于维持细胞完整性和确保细胞信号传导途径的正常运作至关重要，这使得PP1α成为理解磷酸酶活性被破坏的疾病的关键因素。PP1单克隆抗体（E-9）具有广泛的物种反应性，可有效检测小鼠、大鼠和人类样本以及犬、牛和猪样本中的PP1家族催化亚基。PP1（E-9）抗体具有多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。有非结合型和结合型两种形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor ® 结合物，PP1 (E-9) 单克隆抗体通过靶向 PP1 全酶的催化亚基，有助于详细研究 PP1α 在细胞功能和 NIPP-1 等抑制蛋白调节中的作用。了解 PP1α 的功能和调节对于阐明其对细胞信号传导通路的影响以及作为以磷酸酶活性异常为特征的疾病的治疗靶点至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PP1 抗体 (E-9) 参考文献:

  1. 鉴定蛋白磷酸酶 2A 的调节 A 亚基与催化 C 亚基以及猿病毒 40 和多瘤病毒的肿瘤抗原的结合位点。  |  Ruediger, R., et al. 1992. Mol Cell Biol. 12: 4872-82. PMID: 1328865
  2. 亚基相互作用控制蛋白磷酸酶 2A。有限蛋白水解, N-乙基马来酰亚胺和肝素对 B 亚基相互作用的影响。  |  Kamibayashi, C., et al. 1991. J Biol Chem. 266: 13251-60. PMID: 1649186
  3. 蛋白磷酸酶：最新进展。  |  Shenolikar, S. and Nairn, AC. 1991. Adv Second Messenger Phosphoprotein Res. 23: 1-121. PMID: 1847640
  4. 蛋白丝氨酸/苏氨酸磷酸酶；一个不断扩大的家族。  |  Cohen, PT., et al. 1990. FEBS Lett. 268: 355-9. PMID: 2166691
  5. 蛋白磷酸酶的结构和调节。  |  Cohen, P. 1989. Annu Rev Biochem. 58: 453-508. PMID: 2549856
  6. 蛋白磷酸酶：特性和在细胞调控中的作用。  |  Ingebritsen, TS. and Cohen, P. 1983. Science. 221: 331-8. PMID: 6306765
  7. SV40 小肿瘤抗原与蛋白磷酸酶 2A 的相互作用会刺激图谱激酶通路并诱导细胞增殖。  |  Sontag, E., et al. 1993. Cell. 75: 887-97. PMID: 8252625

订购信息

产品名称产品编号规格价格数量收藏夹

PP1 抗体 (E-9)

sc-7482
200 µg/ml
RMB2377.00

PP1 (E-9): m-IgG Fc BP-HRP 套装

sc-528182
200 µg Ab; 10 µg BP
RMB2662.00

PP1 (E-9): m-IgGκ BP-HRP 套装

sc-520503
200 µg Ab, 40 µg BP
RMB2662.00

PP1 (E-9): m-IgG2b BP-HRP 套装

sc-550004
200 µg Ab; 10 µg BP
RMB2662.00

PP1 抗体 (E-9) AC

sc-7482 AC
500 µg/ml, 25% agarose
RMB3129.00

PP1 抗体 (E-9) HRP

sc-7482 HRP
200 µg/ml
RMB2377.00

PP1 抗体 (E-9) FITC

sc-7482 FITC
200 µg/ml
RMB2482.00

PP1 抗体 (E-9) PE

sc-7482 PE
200 µg/ml
RMB2580.00

PP1 抗体 (E-9) Alexa Fluor® 488

sc-7482 AF488
200 µg/ml
RMB2685.00

PP1 抗体 (E-9) Alexa Fluor® 546

sc-7482 AF546
200 µg/ml
RMB2685.00

PP1 抗体 (E-9) Alexa Fluor® 594

sc-7482 AF594
200 µg/ml
RMB2685.00

PP1 抗体 (E-9) Alexa Fluor® 647

sc-7482 AF647
200 µg/ml
RMB2685.00

PP1 抗体 (E-9) Alexa Fluor® 680

sc-7482 AF680
200 µg/ml
RMB2685.00

PP1 抗体 (E-9) Alexa Fluor® 790

sc-7482 AF790
200 µg/ml
RMB2685.00

I have PP1-PE (sc-7482), I will use for Flow Cytometry. At what concentration should I use for 100 ul of washed platelet from 1ml mouse blood? Do you have any recommendation?

Asked by: abva
Thank you for your question. We recommend 1 µg normal immunoglobulins per 1 million cells. Here is our protocol: https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-07-31

PP1抗体 (E-9)可以给动粒染色吗？

Asked by: Mr Wen
感谢您的提问。PP1 抗体 (E-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PP1 family catalytic subunits; 也和以下物种反应，包括： 和 canine, bovine and porcine。 若您的样本不属于以上范围，不能提供质保服务。谢谢。 Thank you for your question. PP1 Antibody (E-9) is recommended for detection of PP1 family catalytic subunits of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine. If your sample does not fall within the above scope, the quality assurance service cannot be provided. Thank you
Answered by: Jiajun Z
Date published: 2023-04-25

Is there an antibody for this protein to detect its phosphorylated form (at Thr 320)?

Asked by: khemeia18
Thank you for your question. We do not offer an antibody specifically against phosphorylated PP1 at residue Thr 320 at this time.
Answered by: Technical Support
Date published: 2019-01-06

How can I ask for a FREE 10 µg sample of PP1 (E-9) (sc-7482)? Thanks.

Asked by: xiaoqin
Thank you for your question. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-09-27

Does this antibody pick up all 3 isoforms (PP1a, PP1b and PP1g)? Or is it recognizing only PP1a?

Asked by: PHVS
Thank you for your question. PP1 Antibody (E-9): sc-7482 is raised against full length phosphatase PP1α of human origin. The exact epitope recognized by this monoclonal antibody has not been determined, so it may recognize all 3 isoforms, but this has not been confirmed experimentally. The antibody was validated by Western blot to recognize PP1 alpha using a specific transfected lysate.
Answered by: Technical Support
Date published: 2017-03-22

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-22
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Rated 4 out of 5 by from Good for Western BlotI used this antibody to check PP1 protein levels in HEK293 cells, and obtained high quality WB results. Used 1:1000 in 0,5% milk. Recommended
Date published: 2024-10-07
Rated 5 out of 5 by from great antibodyI tired this antibody by WB, it works pretty good!
Date published: 2019-11-27
Rated 5 out of 5 by from good productThis antibody works pretty well for western blot in our lab.
Date published: 2018-05-09
Rated 5 out of 5 by from 好用的抗体试用后购买了该产品，WB和IP都很好用！客服非常nice，沟通和回复很及时，希望Santa Cruz的抗体更好用！
Date published: 2017-12-26
Rated 5 out of 5 by from Nice antibody detects endogenous PP1I look for a PP1 antibody that can detect the endogenous protein. This antibody did it job! Highly recommended.
Date published: 2017-11-30
Rated 5 out of 5 by from Great antibodyExcellent signal using 1:1,000 dilution in heart total lysate (WB). Quite clean antibody.
Date published: 2017-09-25
Rated 5 out of 5 by from Clear ressultsVery clear signal and sensitivity to the PP1 positive cells (LNCap cell).1:1000 dilution is used, strong band ,very great result.
Date published: 2017-09-20
Rated 5 out of 5 by from positive resultNo non-specific binding Very clear signal and sensitivity to the PP1 positive cells (A431 cell).1:1000 dilution is used, strong band ,very great result.
Date published: 2017-07-29
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PP1 抗体 (E-9) is rated 4.7 out of 5 by 24.
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