Date published: 2025-9-6
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Polycystin-2 Antibody (YCE2) - Western Blotting - Image 11975
多囊胞素-2 抗体 (YCE2): sc-47734. 对 NRK 全细胞裂解液中 Polycystin-2 的表达进行 Western 印迹分析.
Polycystin-2 Antibody (YCE2) - Western Blotting - Image 11975
多囊胞素-2 抗体 (YCE2): sc-47734. 对 NRK 全细胞裂解液中 Polycystin-2 的表达进行 Western 印迹分析.
Polycystin-2 Antibody (YCE2) - Immunohistochemistry - Image 293410
多囊卵巢蛋白-2 抗体 (YCE2): sc-47734. 福尔马林固定, 石蜡包埋的人输卵管组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
Polycystin-2 Antibody (YCE2) - Western Blotting - Image 354301
Polycystin-2 抗体 (YCE2): sc-47734. Western 印迹分析人乳腺组织提取物中 Polycystin-2 的表达.
多囊胞素-2 抗体 (YCE2): sc-47734. 对 NRK 全细胞裂解液中 Polycystin-2 的表达进行 Western 印迹分析.

Polycystin-2 抗体 (YCE2): sc-47734

4.1(9)
寫評論提問

说明书
  • Polycystin-2 抗体 YCE2 是小鼠单克隆 IgG2a κ，Polycystin-2抗体, 在21篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Polycystin-2的氨基酸687-754
  • Polycystin-2 抗体 (YCE2) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Polycystin-2
  • 抗Polycystin-2抗体（YCE2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Polycystin-2 (YCE2): sc-47734的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Polycystin-2 抗体 (YCE2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Polycystin-2 抗体 (YCE2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Polycystin 抗体 包括 Polycystin-2

多囊肾素-2抗体（YCE2）是一种小鼠单克隆IgG2b kappa轻链抗体，靶向多囊肾素-2，也称为PKD2、TRPP2或PC2，经证实可在人类、大鼠和小鼠样本中通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测产生反应。多囊肾蛋白2是一种膜结合型糖蛋白，主要定位于内质网和细胞膜，在钙离子转运和细胞信号传导中发挥重要作用。多囊肾蛋白2在细胞中的定位非常重要，因为它可以调节钙离子平衡，从而影响细胞的增殖、分化及存活。Polycystin-2的正常功能对于肾脏健康至关重要，PKD2基因的突变与常染色体显性多囊肾（ADPKD）有关，会导致肾小管囊肿的形成。已知Polycystin-2与Polycystin-1相互作用，形成一种复合物，增强AP-1的表达，而AP-1对于调节与细胞生长和凋亡相关的基因表达至关重要。抗多囊肾蛋白-2抗体（YCE2）有非结合抗体和多种结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可用于精确检测和调查多囊肾蛋白-2在肾脏病理学和其他细胞过程中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Polycystin-2 抗体 (YCE2)

sc-47734
200 µg/ml
RMB2377.00

Polycystin-2 (YCE2): m-IgG Fc BP-HRP 套装

sc-525556
200 µg Ab; 10 µg BP
RMB2662.00

Polycystin-2 (YCE2): m-IgG2a BP-HRP 套装

sc-546313
200 µg Ab; 10 µg BP
RMB2662.00

Polycystin-2 抗体 (YCE2) AC

sc-47734 AC
500 µg/ml, 25% agarose
RMB3129.00

Polycystin-2 抗体 (YCE2) HRP

sc-47734 HRP
200 µg/ml
RMB2377.00

Polycystin-2 抗体 (YCE2) FITC

sc-47734 FITC
200 µg/ml
RMB2482.00

Polycystin-2 抗体 (YCE2) PE

sc-47734 PE
200 µg/ml
RMB2580.00

Polycystin-2 抗体 (YCE2) Alexa Fluor® 488

sc-47734 AF488
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (YCE2) Alexa Fluor® 546

sc-47734 AF546
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (YCE2) Alexa Fluor® 594

sc-47734 AF594
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (YCE2) Alexa Fluor® 647

sc-47734 AF647
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (YCE2) Alexa Fluor® 680

sc-47734 AF680
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (YCE2) Alexa Fluor® 790

sc-47734 AF790
200 µg/ml
RMB2685.00

Can positive signals by anti-polycystin-2 (YCE2) be observed in primary cilia?

Asked by: TeddyB
Thank you for your question. Polycystin-2 Antibody (YCE2) is recommended for detection of Polycystin-2 of mouse, rat and human origin by WB, IP, IF and IHC(P). We don't have the information about the expression of polycystin-2 in primary cilia.
Answered by: Technical Service SH
Date published: 2023-02-20

Are there any issues with background when using this antibody to stain mouse tissue?

Asked by: AbPolly
Thank you for your question. This antibody is recommended for use with mouse tissue. In order to avoid any potential background issues with anti-mouse secondaries, we recommend using a directly conjugated version. This antibody is available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 1
Date published: 2017-01-16
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Rated 4 out of 5 by from Band detected in WB using mouse cellBand detected in WB using mouse primary cultured cell.
Date published: 2020-11-30
Rated 1 out of 5 by from Nonspecific and does not detect in some cell typesI have bought two different lots of this antibody, mainly to use for western blotting and IF, along with some immunoprecipitation experiments. I was unable to pull down PC2 with this antibody from liver cells, so I checked via western blot and saw that this antibody showed very nonspecific binding in kidney epithelial (LLC) cells, and did not detect PC2 at all in liver cells (Hg2). I have used antibodies from other companies and have successfully detected PC2 in Hg2 cells, even in the same lysate.
Date published: 2019-12-26
Rated 5 out of 5 by from WB good resultSingle strong band in WB(1/800) in mouse spleen tissue extract, very good.
Date published: 2017-05-31
Rated 5 out of 5 by from good detectionVery nice detect for paraffin-embedded human breast samples,1:500 dilution used.
Date published: 2017-05-28
Rated 5 out of 5 by from good resultsVery good results, the antibody works well for western blot.We use 1/500 dilution in 5% milk TBST.
Date published: 2017-02-25
Rated 5 out of 5 by from Good antibody for IHC(P)Polycystin-2 (YCE2) shows good staining in human fallopian tube for IHC(P), showing cytoplasmic staining of glandular cells.
Date published: 2016-12-22
Rated 4 out of 5 by from IF publishable data with mouse embryosIF publishable data with mouse embryos - SCBT Publication Review
Date published: 2015-05-18
Rated 4 out of 5 by from Band detected in WB using NRK whole cell lysateBand detected in WB using NRK whole cell lysate. -SCBT QC
Date published: 2015-01-02
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Polycystin-2 抗体 (YCE2) is rated 4.1 out of 5 by 9.
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