Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Polycystin-2 Antibody (D-3) - Western Blotting - Image 354314
多囊胞素-2 抗体 (D-3): sc-28331. NIH/3T3 (A), 3T3-L1 (B) 和 RAT2 (C) 全细胞裂解液中聚胞素-2 表达的 Western 印迹分析.
多囊胞素-2 抗体 (D-3): sc-28331. NIH/3T3 (A), 3T3-L1 (B) 和 RAT2 (C) 全细胞裂解液中聚胞素-2 表达的 Western 印迹分析.

Polycystin-2 抗体 (D-3): sc-28331

4.6(10)
寫評論提問

说明书
  • Polycystin-2抗体(D-3)是小鼠单克隆IgG2b κ, 在33篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Polycystin-2的氨基酸689-968
  • 抗-Polycystin-2 抗体 (D-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Polycystin-2
  • 抗Polycystin-2抗体（D-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Polycystin-2 (D-3): sc-28331的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Polycystin-2 抗体 (D-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Polycystin-2 抗体 (D-3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Polycystin 抗体 包括 Polycystin-2

多囊肾蛋白2抗体（D-3）是一种小鼠单克隆IgG2b kappa轻链抗体，专门针对多囊肾蛋白2（又称PKD2），在人类、大鼠和小鼠来源中均具有反应性，可用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）在内的各种应用。多囊肾蛋白2是一种重要的跨膜蛋白，参与钙信号传导，对于维持正常肾功能至关重要，因为PKD2可调节细胞内的离子运输。多囊肾蛋白2的缺陷与常染色体显性遗传性多囊肾（ADPKD）有关，其中突变会导致肾小管内囊肿的形成，从而导致肾功能逐渐衰竭。PKD2主要定位于肾小管和皮质集合管以及远曲小管等肾脏结构内，并与Polycystin-1相互作用，调节AP-1信号，影响细胞的增殖、分化及凋亡。通过与Hax-1结合，Polycystin-2还可能影响细胞与基质的相互作用，进一步支持细胞的稳定性和功能。Polycystin-2 (D-3) 抗体有非结合型或多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 以及各种 Alexa Fluor® 选项，可用于检测和研究 PKD2 在 ADPKD 和其他细胞过程中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Polycystin-2 抗体 (D-3) 参考文献:

  1. 常染色体显性多囊肾病基因产物 PKD2 引发的细胞活化。  |  Arnould, T., et al. 1999. Mol Cell Biol. 19: 3423-34. PMID: 10207066
  2. 常染色体显性多囊肾病蛋白--多囊卵巢蛋白-2 和多囊卵巢蛋白-1 在正常组织和囊肿组织中的协调表达。  |  Ong, AC., et al. 1999. Am J Pathol. 154: 1721-9. PMID: 10362797
  3. PKD2基因产物多囊胞素-2的鉴定和特征描述。  |  Cai, Y., et al. 1999. J Biol Chem. 274: 28557-65. PMID: 10497221
  4. Polycystin-1是PKD1基因的产物，与E-cadherin和catenins组成复合物。  |  Huan, Y. and van Adelsberg, J. 1999. J Clin Invest. 104: 1459-68. PMID: 10562308
  5. 大鼠 pkd2 蛋白在不同器官中有不同的亚细胞分布。  |  Obermüller, N., et al. 1999. Am J Physiol. 277: F914-25. PMID: 10600939
  6. Pkd2 靶向突变小鼠的心脏缺陷和肾衰竭  |  Wu, G., et al. 2000. Nat Genet. 24: 75-8. PMID: 10615132
  7. 多囊肾病蛋白 PKD2 与 Hax-1 相互作用，Hax-1 是一种与肌动蛋白细胞骨架缔合作用的蛋白。  |  Gallagher, AR., et al. 2000. Proc Natl Acad Sci U S A. 97: 4017-22. PMID: 10760273
  8. 多囊肾病 1 基因产物介导蛋白激酶 C alpha 依赖性和 c-Jun N 端激酶依赖性激活转录因子 AP-1。  |  Arnould, T., et al. 1998. J Biol Chem. 273: 6013-8. PMID: 9497315

订购信息

产品名称产品编号规格价格数量收藏夹

Polycystin-2 抗体 (D-3)

sc-28331
200 µg/ml
RMB2377.00

Polycystin-2 (D-3): m-IgG Fc BP-HRP 套装

sc-528427
200 µg Ab; 10 µg BP
RMB2662.00

Polycystin-2 (D-3): m-IgGκ BP-HRP 套装

sc-520801
200 µg Ab, 40 µg BP
RMB2662.00

Polycystin-2 (D-3): m-IgG2b BP-HRP 套装

sc-550036
200 µg Ab; 10 µg BP
RMB2662.00

Polycystin-2 抗体 (D-3) AC

sc-28331 AC
500 µg/ml, 25% agarose
RMB3129.00

Polycystin-2 抗体 (D-3) HRP

sc-28331 HRP
200 µg/ml
RMB2377.00

Polycystin-2 抗体 (D-3) FITC

sc-28331 FITC
200 µg/ml
RMB2482.00

Polycystin-2 抗体 (D-3) PE

sc-28331 PE
200 µg/ml
RMB2580.00

Polycystin-2 抗体 (D-3) Alexa Fluor® 488

sc-28331 AF488
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (D-3) Alexa Fluor® 546

sc-28331 AF546
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (D-3) Alexa Fluor® 594

sc-28331 AF594
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (D-3) Alexa Fluor® 647

sc-28331 AF647
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (D-3) Alexa Fluor® 680

sc-28331 AF680
200 µg/ml
RMB2685.00

Polycystin-2 抗体 (D-3) Alexa Fluor® 790

sc-28331 AF790
200 µg/ml
RMB2685.00

Hello, I would like to use a blocking peptide to preadsorb an antibody (sc-28331) as a negative control. We are interested in synthesizing a peptide to do this using the immunogen sequence. How were the immunogen peptides for this antibody produced?

Asked by: Chris
Thank you for your question. The immunogen is proprietary but is is a link for more information on the protein. https://www.uniprot.org/uniprotkb/Q13563/entry
Answered by: BlakeJ
Date published: 2025-01-08

Can you address the discrepancy between your datasheets for the D3 and YCE2 antibodies? The sheet for YCE2 lists PC2 molecular weight as 110 kDa, consistent with current research. However, D3 sheet says 130 kDa, and the example image supports it-- how?

Asked by: Klab
Thank you for bringing this discrepancy on our product data sheets to our attention. We are going to update them to be consistent. According to Uniprot, and the literature, the predicted molecular weight for isoform 1 of Polycystin-2 is ~ 110 kDa. However, we typically observe a higher molecular weight ~ 130-134 kDa. This may be due to post-translational modification of the protein as we are aware that Polycystin-2 is glycosylated.
Answered by: Technical Support
Date published: 2020-04-28

Can you provide with a FACS protocol for this antibody (polycystin-2, D3, sc-28331) on cell lines?

Asked by: Curious2
Thank you for your question. We do not have a specific FACS protocol for use with this antibody. Please check our general protocols online, or contact our Technical Support for more information.
Answered by: Technical Support
Date published: 2019-04-13

I am using Polycystin-2 (D-3): sc-28331 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_28331, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 169ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Clear and specific antibody (WB)I use this for WB, and can get clear, specific band.
Date published: 2020-11-30
Rated 5 out of 5 by from Nice IHC-P resultswhen used with heat (sodium citrate) antigen retrieval at 4ug/ml concentration on human tissue
Date published: 2017-12-19
Rated 5 out of 5 by from Clear, specific antibodyThe polycystin-2 D-3 antibody gives very clean, specific bands in western blots of protein from C2C12 cells. Our lab has also used it for immunofluorescence with good results. We'll be purchasing more of this antibody!
Date published: 2017-09-21
Rated 5 out of 5 by from Excellent!This antibody is great in Western blot analysis of Polycystin-2 expression in NIH/3T3, 3T3-L1 and RAT2 whole cell lysates.
Date published: 2017-01-27
Rated 5 out of 5 by from Band detected in WB using CakiBand detected in WB using Caki-1 whole cell lysate. -SCBT QC
Date published: 2015-06-30
Rated 4 out of 5 by from Publishable data in ICC using mouse basalPublishable data in ICC using mouse basal body and/or centrosome -SCBT Publication Review
Date published: 2015-05-29
Rated 5 out of 5 by from Publishable data in WB using Human PDL fibroblastsPublishable data in WB using Human PDL fibroblasts -SCBT Publication Review
Date published: 2015-04-15
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-15
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_28331, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
Polycystin-2 抗体 (D-3) is rated 4.6 out of 5 by 10.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_28331, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 122ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。