Date published: 2025-9-7
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Pol II Antibody (F-12) - Western Blotting - Image 353139
Pol II 抗体 (F-12): sc-55492. P19 (A), A-431 (B) 和 HeLa (C) 全细胞裂解液中 Pol II 表达的 Western 印迹分析.
Pol II 抗体 (F-12): sc-55492. P19 (A), A-431 (B) 和 HeLa (C) 全细胞裂解液中 Pol II 表达的 Western 印迹分析.

Pol II 抗体 (F-12): sc-55492

4.8(9)
寫評論提問

说明书
  • Pol II抗体(F-12)是小鼠单克隆IgG2b κ, 在61篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Pol II的氨基酸1-224
  • 抗-Pol II 抗体 (F-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Pol II
  • 抗Pol II抗体（F-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Pol II (F-12): sc-55492的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 Pol II 抗体 (F-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Pol II 抗体 (F-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 RNA Polymerase 抗体包括 RNA pol α, Pol II, Pol II RPB5, Pol II RPB6, Pol III RPC32, Pol III RPC39, RNA pol σ, RPA135, RPA16 和 RPA194

Pol II 抗体 (F-12) 是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过各种应用检测小鼠、大鼠和人类样本中的 RNA 聚合酶 II (PolⅡ)，包括免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组织化学和酶联免疫吸附试验 (ELISA)。RNA 聚合酶 II (Pol II) 抗体 (F-12) 有非共轭和多种共轭形式，如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物。RNA 聚合酶 II 在转录蛋白质编码基因中起着至关重要的作用，RNA 聚合酶 II 的功能对基因表达调控至关重要。RNA 聚合酶 II 由 12 个亚基组成，其中 RPB1（又称 RPB205）是一种 1,840 氨基酸的蛋白质，具有一个 C2H2 型锌指和一个富含七肽重复的 C 端结构域。RPB1 对 RNA 聚合酶 II 的催化活性至关重要，并形成负责合成信使 RNA 的活性中心。RPB1 还有助于 RNA 聚合酶 II 的 DNA 结合域，形成准确转录 DNA 模板所需的沟槽。RPB1 功能障碍会严重影响 mRNA 合成并阻碍转录延伸，从而凸显 RNA 聚合酶 II 在细胞功能和基因调控中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Pol II 抗体 (F-12)

sc-55492
200 µg/ml
RMB2377.00

Pol II (F-12): m-IgG Fc BP-HRP 套装

sc-525627
200 µg Ab; 10 µg BP
RMB2662.00

Pol II (F-12): m-IgG2b BP-HRP 套装

sc-549597
200 µg Ab; 10 µg BP
RMB2662.00

Pol II 抗体 (F-12) AC

sc-55492 AC
500 µg/ml, 25% agarose
RMB3129.00

Pol II 抗体 (F-12) HRP

sc-55492 HRP
200 µg/ml
RMB2377.00

Pol II 抗体 (F-12) FITC

sc-55492 FITC
200 µg/ml
RMB2482.00

Pol II 抗体 (F-12) PE

sc-55492 PE
200 µg/ml
RMB2580.00

Pol II 抗体 (F-12) Alexa Fluor® 488

sc-55492 AF488
200 µg/ml
RMB2685.00

Pol II 抗体 (F-12) Alexa Fluor® 546

sc-55492 AF546
200 µg/ml
RMB2685.00

Pol II 抗体 (F-12) Alexa Fluor® 594

sc-55492 AF594
200 µg/ml
RMB2685.00

Pol II 抗体 (F-12) Alexa Fluor® 647

sc-55492 AF647
200 µg/ml
RMB2685.00

Pol II 抗体 (F-12) Alexa Fluor® 680

sc-55492 AF680
200 µg/ml
RMB2685.00

Pol II 抗体 (F-12) Alexa Fluor® 790

sc-55492 AF790
200 µg/ml
RMB2685.00

Can this antibody be ordered at the sc-514935-X (200ug/0.1mL)?

Asked by: Simon
Thank you for your question. Please contact our technical service team directly to request custom formulation products.
Answered by: Tech Support Europe
Date published: 2024-04-23

Is Pol II Antibody (F-12): sc-55492 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Pol II (CTD4H8), which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/pol-ii-antibody-ctd4h8)
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 5 out of 5 by from Great antibodyWe used this antibody in Chromatin immunoprecipitation experiments and it works very well
Date published: 2023-12-13
Rated 5 out of 5 by from Reliable tool for research!One of the main and most reliable antibodies for my research!
Date published: 2018-01-18
Rated 5 out of 5 by from Good for WBThis antibody is good for Western blot with human cell nuclear extract.
Date published: 2017-12-30
Rated 4 out of 5 by from Okay antibodyDidn't get clear bands like in the datasheet, rather got some smearing of signal.
Date published: 2017-08-22
Rated 5 out of 5 by from Superb !!Western blot and staining results are so much better than polyclonal alternatives
Date published: 2017-01-10
Rated 5 out of 5 by from Reliable Antibody for Many ApplicationsThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Very reliable.
Date published: 2016-12-22
Rated 5 out of 5 by from IF: good nuclear staining with HeLa cellsIF: good nuclear staining with HeLa cells -SCBT QC
Date published: 2015-04-15
Rated 4 out of 5 by from Works well in WB with AWorks well in WB with A-673 and NIH/3T3 nuclear extracts and AT-3 whole cell lysate -SCBT QC
Date published: 2015-03-10
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Pol II 抗体 (F-12) is rated 4.8 out of 5 by 9.
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