Date published: 2025-9-7
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Pol II Antibody (8WG16) - Western Blotting - Image 17625
Pol II 抗体 (8WG16): sc-56767. A-431 (A), A-673 (B), HeLa (C) 和 NIH/3T3 (D) 核提取物及 HeLa 全细胞裂解液 (E) 中 Pol II 表达的 Western 印迹分析.
Pol II Antibody (8WG16) - Western Blotting - Image 17625
Pol II 抗体 (8WG16): sc-56767. A-431 (A), A-673 (B), HeLa (C) 和 NIH/3T3 (D) 核提取物及 HeLa 全细胞裂解液 (E) 中 Pol II 表达的 Western 印迹分析.
Pol II Antibody (8WG16) - Western Blotting - Image 392889
Pol II 抗体 (8WG16) HRP: sc-56767 HRP. 对 A-431 (A), A-673 (B) 和 HeLa (C) 核提取物以及 HeLa (D), HeLa + Calyculin A (E) 和 PC-12 (F) 全细胞裂解液中 Pol II 表达的直接 Western 印迹分析.
Pol II 抗体 (8WG16): sc-56767. A-431 (A), A-673 (B), HeLa (C) 和 NIH/3T3 (D) 核提取物及 HeLa 全细胞裂解液 (E) 中 Pol II 表达的 Western 印迹分析.

Pol II 抗体 (8WG16): sc-56767

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说明书
  • Pol II抗体(8WG16)是小鼠单克隆IgG2a κ, 在104篇文献中引用，规格为200 µg/ml
  • 针对wheat germ来源的纯化RNA聚合酶II
  • 抗-Pol II 抗体 (8WG16) 推荐用于 WB, IP 和 IF，检测mouse, rat, human, Xenopus, C. elegans, yeast, wheat germ 和bovine 来源的 the highly conserved heptapeptide repeat of the largest subunit of eukaryotic Pol II
  • 抗Pol II抗体（8WG16）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Pol II (8WG16): sc-56767的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Pol II Antibody (8WG16) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Pol II Antibody (8WG16) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加RNA Polymerase抗体，包括RNA pol α, Pol II, Pol II RPB5, Pol II RPB6, Pol III RPC32, Pol III RPC39, RNA pol σ, RPA135, RPA16, RPA194, RPA40, Pol I/II/III RPB6和Pol I/II/III RPB8

Pol II 抗体 (8WG16) 是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过 Western 印迹 (WB)、免疫沉淀 (IP) 和免疫荧光 (IF) 等应用检测不同物种的 RNA 聚合酶 II (PolⅡ)，包括小鼠、大鼠、人、爪蟾、优雅子、酵母、小麦胚芽和牛。RNA 聚合酶 II (Pol II) 抗体 (8WG16) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物。RNA 聚合酶 II 是一种重要的酶，由十二个亚基组成，负责转录编码蛋白质的基因，对基因表达和细胞功能至关重要。RNA 聚合酶 II 的最大亚基称为 RPB1 或 RPB205，是一种 1,840 个氨基酸的蛋白质，具有一个 C2H2- 型锌指和一个富含七肽重复的 C 端结构域。这种结构组成至关重要，因为 RNA 聚合酶 II 不仅能促进催化活性，还在形成 DNA 结合域方面发挥着关键作用，而 DNA 结合域是准确转录 DNA 模板所必需的。最大亚基的完整性至关重要；没有正常的功能，mRNA 合成和转录延伸过程就会受到严重影响，这凸显了 RNA 聚合酶 II 在维持细胞平衡和调节基因表达方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pol II 抗体 (8WG16) 参考文献:

  1. 转录的结构基础：4.5 埃的 RNA 聚合酶 II-TFIIB 共晶体。  |  Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
  2. RNA 聚合酶 II 的结构和功能。  |  Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
  3. RNA 聚合酶 I 的转录机制。  |  Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
  4. RNA 聚合酶 II 的转录周期：在染色质中循环。  |  Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
  5. RNA 聚合酶 III 转录与癌症  |  White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
  6. BRCA1 与 NUFIP 和 P-TEFb 合作，激活 RNA 聚合酶 II 的转录。  |  Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
  7. 表皮生长因子可提高细胞中 TATA 结合蛋白的水平，并诱导 RNA 聚合酶 I 和 III 依赖性基因的活性。  |  Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
  8. 视网膜母细胞瘤肿瘤抑制蛋白抑制 RNA 聚合酶 III 转录的不同机制  |  Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
  9. 下游 DNA 选择性地影响人类 RNA 聚合酶 II 的暂停构象。  |  Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834

订购信息

产品名称产品编号规格价格数量收藏夹

Pol II 抗体 (8WG16)

sc-56767
200 µg/ml
RMB2504.00

Pol II (8WG16): m-IgG Fc BP-HRP 套装

sc-537117
200 µg Ab; 10 µg BP
RMB2662.00

Pol II (8WG16): m-IgGκ BP-HRP 套装

sc-534309
200 µg Ab; 40 µg BP
RMB2662.00

Pol II (8WG16): m-IgG2a BP-HRP 套装

sc-547929
200 µg Ab; 10 µg BP
RMB2662.00

Pol II 抗体 (8WG16) AC

sc-56767 AC
500 µg/ml, 25% agarose
RMB3129.00

Pol II 抗体 (8WG16) HRP

sc-56767 HRP
200 µg/ml
RMB2377.00

Pol II 抗体 (8WG16) FITC

sc-56767 FITC
200 µg/ml
RMB2482.00

Pol II 抗体 (8WG16) PE

sc-56767 PE
200 µg/ml
RMB2580.00

Pol II 抗体 (8WG16) Alexa Fluor® 488

sc-56767 AF488
200 µg/ml
RMB2685.00

Pol II 抗体 (8WG16) Alexa Fluor® 546

sc-56767 AF546
200 µg/ml
RMB2685.00

Pol II 抗体 (8WG16) Alexa Fluor® 594

sc-56767 AF594
200 µg/ml
RMB2685.00

Pol II 抗体 (8WG16) Alexa Fluor® 647

sc-56767 AF647
200 µg/ml
RMB2685.00

Pol II 抗体 (8WG16) Alexa Fluor® 680

sc-56767 AF680
200 µg/ml
RMB2685.00

Pol II 抗体 (8WG16) Alexa Fluor® 790

sc-56767 AF790
200 µg/ml
RMB2685.00

Could I get a bulk quote for 1 mg 8WG16?

Asked by: drs808
Thank you for contacting Santa Cruz Biotechnology. For a price quote please email [email protected].
Answered by: BlakeJ
Date published: 2024-11-22

which subunit of pol II does this antibody recognize? The datasheet says the heptapeptide repeat of the largest subunit of eukaryotic Pol II, but it doesn't say if it's the subunit A, B, C, D, etc. Which one is it? Thanks.

Asked by: john
Thank you for your question. Subunit A and B can be detected . https://www.uniprot.org/uniprotkb/P08775/entry
Answered by: BlakeJ
Date published: 2024-03-28

I would like to pull down RNA polymerase II from Nicotiana benthamiana. Will the Pol II Antibody (8WG16) AC work ?

Asked by: Bera
Thank you for your question. It is possible that the AC form would work . Please contact technical service at [email protected] or (800)-457-3801. Please provide the accession number for your sample.
Answered by: BlakeJ
Date published: 2023-05-03

How much Ab is in every tube? The datasheet states "100µg in 1mL PBS per tube", whereas the product description only gives a concentration (200µg/mL). And they are different! Thanks

Asked by: JR Riquelme
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Will this antibody work against Drosophila ovary sample ?

Asked by: Raksha
Thank you for your question. Pol II Antibody (8WG16): sc-56767 is specific against the highly conserved heptapeptide repeat of the largest subunit of eukaryotic Pol II. It has already been used in a publication that worked with samples from Drosophila: PMID 19073139 (ChIP).
Answered by: Tech Support Europe
Date published: 2020-06-25

Is this antibody known to interact with RNAPII regardless of its phosphorylation state?

Asked by: yeet
Thank you for your question. Yes, this antibody will detect Pol II regardless of phosphorylation state.
Answered by: Tech Service
Date published: 2019-08-18

I want to detect Poll II in my monkey samples. Will this antibody work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-29
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Rated 5 out of 5 by from WB detectionTested for WB in a 1:1000 dilution on mouse kidney tissue cells. This antibody showed a fine band for PolII at the correct size at about 210kda.
Date published: 2017-07-23
Rated 5 out of 5 by from GoodThe antibody was good, and the results were good in the western blot experiment, very good.
Date published: 2017-02-12
Rated 5 out of 5 by from Performance is very goodThe antibodies is very good, used in the experiment the Pol II (8 wg16) monoclonal antibody test conclusion satisfactory, great products to recommend!
Date published: 2017-02-10
Rated 4 out of 5 by from 8WG16- ExcelenteFunciona fantasticamente para Western de extractos, IP y ChIp.
Date published: 2017-02-01
Rated 5 out of 5 by from unluckyAccording to the textbook, the largest subunit of Pol II (called RPB1 or RPB205) is a 1,840 amino acid protein that contains a C2H2 zinc finger and a C-terminal domain consisting of several heptad repeats. We tried to test this conclusion, but failed, but the product characteristics are still good.
Date published: 2017-01-24
Rated 5 out of 5 by from Recommended for wide range of species typePol II (8WG16) monoclonal antibody is great to use with various samples types, such as mouse, human, Xenopus, C. elegans, yeast, wheat germ and bovine
Date published: 2016-12-20
Rated 5 out of 5 by from good for ChIPThis antibody works for ChIP in mouse embryonic fibroblast, good replacement for sc-899
Date published: 2016-11-28
Rated 5 out of 5 by from Various publications list success using thisVarious publications list success using this antibody in ChIP applications utilizing mouse T435D, MEF cells and mouse BM macrophages. -SCBT Publication Review
Date published: 2015-05-19
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Pol II 抗体 (8WG16) is rated 4.8 out of 5 by 14.
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