Date published: 2025-9-7
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Pol II Antibody (CTD4H8) - Western Blotting - Image 356692
Pol II 抗体 (CTD4H8): sc-47701. A-431 (A), 3T3-L1 (B), A-673 (C), U-2 OS (D) 和 AT3B-1 (E) 全细胞裂解液中 Pol II 表达的 Western 印迹分析.
Pol II 抗体 (CTD4H8): sc-47701. A-431 (A), 3T3-L1 (B), A-673 (C), U-2 OS (D) 和 AT3B-1 (E) 全细胞裂解液中 Pol II 表达的 Western 印迹分析.

Pol II 抗体 (CTD4H8): sc-47701

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寫評論提問

说明书
  • Pol II 抗体 CTD4H8 是小鼠单克隆 IgG1 κ，Pol II抗体, 在112篇文献中引用，规格为200 µg/ml
  • 针对10个重复的合成肽YSPTSPS使用化学合成的磷酸化丝氨酸5
  • Pol II 抗体 (CTD4H8) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 hypo- and hyper-phosphorylated Pol II
  • 抗Pol II抗体（CTD4H8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-47701 X, 200 µg/0.1 ml)
  • 关于如何获取Pol II (CTD4H8): sc-47701的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Pol II 抗体 (CTD4H8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Pol II 抗体 (CTD4H8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 RNA Polymerase 抗体包括 RNA pol α, Pol II, Pol II RPB5, Pol II RPB6, Pol III RPC32, Pol III RPC39, RNA pol σ, RPA135, RPA16 和 RPA194

Pol II抗体（CTD4H8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的Pol II蛋白。抗Pol II抗体（CTD4H8）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。RNA聚合酶II（Pol II）在蛋白质编码基因的转录中起着至关重要的作用，而Pol II的功能对于基因表达调控至关重要。该酶由12个亚基组成，其中最大的亚基RPB1（也称为RPB205）是一种1840个氨基酸的蛋白质，具有C2H2型锌指和富含七肽重复的C末端结构域。RPB1的结构完整性至关重要，因为它不仅传递Pol II的催化活性，还参与DNA结合域的形成，而DNA结合域对于转录过程至关重要。RPB1与其他亚基之间的相互作用确保了转录机制能够有效地被招募到目标启动子，从而促进mRNA合成的开始。Pol II功能的破坏，特别是通过改变最大的亚基，会导致基因表达的严重损害，这强调了RPB1在细胞功能和发育中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Pol II 抗体 (CTD4H8)

sc-47701
200 µg/ml
RMB2377.00

Pol II (CTD4H8): m-IgG Fc BP-HRP 套装

sc-526732
200 µg Ab; 10 µg BP
RMB2662.00

Pol II (CTD4H8): m-IgG1 BP-HRP 套装

sc-532105
200 µg Ab; 20 µg BP
RMB2662.00

Pol II 抗体 (CTD4H8) X

sc-47701 X
200 µg/0.1 ml
RMB2377.00

Pol II 抗体 (CTD4H8) AC

sc-47701 AC
500 µg/ml, 25% agarose
RMB3129.00

Pol II 抗体 (CTD4H8) HRP

sc-47701 HRP
200 µg/ml
RMB2377.00

Pol II 抗体 (CTD4H8) FITC

sc-47701 FITC
200 µg/ml
RMB2482.00

Pol II 抗体 (CTD4H8) PE

sc-47701 PE
200 µg/ml
RMB2580.00

Pol II 抗体 (CTD4H8) Alexa Fluor® 488

sc-47701 AF488
200 µg/ml
RMB2685.00

Pol II 抗体 (CTD4H8) Alexa Fluor® 546

sc-47701 AF546
200 µg/ml
RMB2685.00

Pol II 抗体 (CTD4H8) Alexa Fluor® 594

sc-47701 AF594
200 µg/ml
RMB2685.00

Pol II 抗体 (CTD4H8) Alexa Fluor® 647

sc-47701 AF647
200 µg/ml
RMB2685.00

Pol II 抗体 (CTD4H8) Alexa Fluor® 680

sc-47701 AF680
200 µg/ml
RMB2685.00

Pol II 抗体 (CTD4H8) Alexa Fluor® 790

sc-47701 AF790
200 µg/ml
RMB2685.00

With what kind of magnetic beads these Abs work good for ChIP? I tried with Dynabeads Protein G and I didn't get it

Asked by: Asya
Thank you for your question. We have not specifically tested this antibody using magnetic beads, and we do not sell those type of magnetic beads so we can not make a recommendation. This is a mouse monoclonal IgG1, so we would recommend asking the magnetic bead supplier for their recommendation. This specific monoclonal antibody has been validated for ChIP (please refer to the product citations for more details).
Answered by: Technical Support
Date published: 2019-01-26

According to the info sheet, this antibody was RNAPII Ser5p specific, have you check the specificity against Ser2p/Ser7p or non phopsphorylated RNAPII?

Asked by: Babybear
Thank you for your question. This antibody is recommended for detection of hypo- and hyper-phosphorylated Pol II of mouse, rat and human origin.
Answered by: Tech Service
Date published: 2024-11-12

Can I use this mouse monoclonal antibody for IHC or IF staining on mouse samples?

Asked by: Anna99
Yes, this Pol II Antibody (CTD4H8): sc-47701 is available conjugated to agarose (sc-47701 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-47701 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-47701 PE), fluorescein (sc-47701 FITC), Alexa Fluor® 488 (sc-47701 AF488) or Alexa Fluor® 647 (sc-47701 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2017-01-05

Does the antibody specifically recognizes only Ser5-phosphorylated Pol II CTD? I would like to perform ChIP for total Pol II, as well as Ser5- and Ser2-phosphorylated Pol II. Would you recommend using this antibody as a control for total Pol II?

Asked by: Mauri
  • y_2025, m_9, d_6, h_5CST
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Rated 5 out of 5 by from works well for ChIPWorks well for ChIP and ChIP-seq (human MCF10A cells)
Date published: 2023-03-22
Rated 5 out of 5 by from Easy to UsePurchased this antibody for use in immunofluorescence and microscopy. Antibody worked well in a variety of concentrations and when paired with a secondary produced good images.
Date published: 2020-08-06
Rated 5 out of 5 by from worked greatat 1:1000 dilution the Western blot worked first time
Date published: 2019-11-01
Rated 4 out of 5 by from It works well for ChIPWe tried this antibody for ChIP on both isolated primary mouse hepatocytes and mouse livers, and it worked well!
Date published: 2019-07-07
Rated 4 out of 5 by from Works for ChIPThsi antibody Works well for Chip, for both cell culture and mouse livers.
Date published: 2019-05-07
Rated 5 out of 5 by from High Signal, no backgroundpPolII antibody was tested on 20µg murine Chromatin. Antibody detected the correct band with no backgorund. We use the antibody 1:1000. No complains!
Date published: 2018-04-19
Rated 3 out of 5 by from Clean but low signalI got a trail size of this antibody and tested it on Murine erythroleukemia cells (MEL). I got clean sharp bands on Western blot, but the signal was very low. I didn't quantify the amount of protein, but the minimum lysate required to see a signal was roughly equivalent to that from 150,000 cells. IMO for Pol II that's a lot!
Date published: 2018-02-23
Rated 4 out of 5 by from Work for HEK cells CHIP!It worked for HEK cells CHIP experiment! It actually worked!
Date published: 2018-02-14
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Pol II 抗体 (CTD4H8) is rated 4.4 out of 5 by 28.
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