Date published: 2025-9-7
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Pol II Antibody (A-10) - Western Blotting - Image 380573
Pol II 抗体 (A-10): sc-17798. A-431 (A), A-673 (B) 和 NIH/3T3 (C) 全细胞裂解液中 Pol II 表达的 Western 印迹分析.
Pol II 抗体 (A-10): sc-17798. A-431 (A), A-673 (B) 和 NIH/3T3 (C) 全细胞裂解液中 Pol II 表达的 Western 印迹分析.

Pol II 抗体 (A-10): sc-17798

4.6(12)
寫評論提問

说明书
  • Pol II抗体(A-10)是小鼠单克隆IgG2b κ, 在48篇文献中引用，规格为200 µg/ml
  • 免疫human物种的RNA Pol II的氨基酸1-224
  • 抗-Pol II 抗体 (A-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Pol II
  • 抗Pol II抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Pol II (A-10): sc-17798的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Pol II Antibody (A-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Pol II Antibody (A-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外RNA Polymerase抗体，包括RNA pol α, Pol II, Pol II RPB5, Pol II RPB6, Pol III RPC32, Pol III RPC39, RNA pol σ, RPA135, RPA16, RPA194, RPA40, Pol I/II/III RPB6和Pol I/II/III RPB8

Pol II抗体（A-10）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类样本中的RNA聚合酶II（Pol II）。 酶联免疫吸附试验（ELISA）。抗RNA聚合酶II抗体（A-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合型。RNA聚合酶II在蛋白质编码基因的转录中起着至关重要的作用，其正常功能对于基因表达调控至关重要。RNA聚合酶II由12个亚基组成，其中RPB1（也称为RPB205）是一种含有1840个氨基酸的蛋白质，具有C2H2型锌指和富含七肽重复的C末端结构域。RPB1对于催化活性至关重要，并形成DNA模板转录所需的DNA结合结构域。 RPB1与第二大亚基的相互作用对于形成RNA聚合酶II的活性中心至关重要，功能障碍会导致mRNA合成和转录延长受损。 Pol II (A-10)单克隆抗体是研究人员在各种生物学背景下研究转录调控和基因表达分子机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Pol II 抗体 (A-10)

sc-17798
200 µg/ml
RMB2377.00

Pol II (A-10): m-IgGκ BP-HRP 套装

sc-520662
200 µg Ab, 40 µg BP
RMB2662.00

Pol II (A-10): m-IgG2b BP-HRP 套装

sc-548845
200 µg Ab; 10 µg BP
RMB2662.00

Pol II 抗体 (A-10) AC

sc-17798 AC
500 µg/ml, 25% agarose
RMB3129.00

Pol II 抗体 (A-10) HRP

sc-17798 HRP
200 µg/ml
RMB2377.00

Pol II 抗体 (A-10) FITC

sc-17798 FITC
200 µg/ml
RMB2482.00

Pol II 抗体 (A-10) PE

sc-17798 PE
200 µg/ml
RMB2580.00

Pol II 抗体 (A-10) Alexa Fluor® 488

sc-17798 AF488
200 µg/ml
RMB2685.00

Pol II 抗体 (A-10) Alexa Fluor® 546

sc-17798 AF546
200 µg/ml
RMB2685.00

Pol II 抗体 (A-10) Alexa Fluor® 594

sc-17798 AF594
200 µg/ml
RMB2685.00

Pol II 抗体 (A-10) Alexa Fluor® 647

sc-17798 AF647
200 µg/ml
RMB2685.00

Pol II 抗体 (A-10) Alexa Fluor® 680

sc-17798 AF680
200 µg/ml
RMB2685.00

Pol II 抗体 (A-10) Alexa Fluor® 790

sc-17798 AF790
200 µg/ml
RMB2685.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Pol II Antibody (A-10): sc-17798?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 5 out of 5 by from Pol II antibody works well for ChIP-qPCR in humanPol II antibody works well for ChIP-qPCR in human cell lines. https://www.pnas.org/doi/10.1073/pnas.1602886113
Date published: 2025-02-16
Rated 4 out of 5 by from GoodThe band is quite strong, but some backdround also present.
Date published: 2021-05-31
Rated 4 out of 5 by from Good signal on WBFast detection, good signal, expected size, can recommend
Date published: 2020-01-16
Rated 3 out of 5 by from Works for WBThis antibody works for Western blot at 1:200 dilution using human cell whole cell lysate. Not great.
Date published: 2018-02-21
Rated 5 out of 5 by from good antibodyThe antibody is well-quoted in a variety of applications with little background. In the observation process clearly, can be a good observation and guidance, and ultimately helped my test success
Date published: 2017-01-18
Rated 5 out of 5 by from Strong WB !!Very strong western blot data; also see CTD4H8 Clone
Date published: 2017-01-11
Rated 5 out of 5 by from Great antibody for WesternsThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background.
Date published: 2016-12-30
Rated 4 out of 5 by from Used in WBUsed in WB. Optimal results at a 1:300.
Date published: 2015-04-08
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Pol II抗体 (A-10) is rated 4.6 out of 5 by 12.
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