Date published: 2025-9-7
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Pol II Antibody (3WG2) - Western Blotting - Image 8681
Pol II 抗体 (3WG2): sc-21750. A-431 (A), U-2 OS (B), NIH/3T3 (C) 和 PC-12 (D) 全细胞裂解液中 Pol II 表达的 Western 印迹分析.
Pol II 抗体 (3WG2): sc-21750. A-431 (A), U-2 OS (B), NIH/3T3 (C) 和 PC-12 (D) 全细胞裂解液中 Pol II 表达的 Western 印迹分析.

Pol II 抗体 (3WG2): sc-21750

5.0(2)
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说明书
  • Pol II抗体(3WG2)是小鼠单克隆IgM κ, 在7篇文献中引用，规格为200 µg/ml
  • 针对RNA聚合酶II最大亚基上保守的七肽重复
  • 推荐用于 mouse, rat 和 human 来源的Pol II WB 和 IP检测
  • 看 Pol II (CTD4H8): sc-47701，可查看有标记的 Pol II 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP是Pol II Antibody (3WG2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Pol II Antibody (3WG2) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

额外RNA Polymerase抗体，包括RNA pol α, Pol II, Pol II RPB5, Pol II RPB6, Pol III RPC32, Pol III RPC39, RNA pol σ, RPA135, RPA16, RPA194, RPA40, Pol I/II/III RPB6和Pol I/II/III RPB8

RNA聚合酶II（Pol II）是一种由12个亚基组成的酶，负责蛋白质编码基因的转录。转录起始需要Pol II介导的转录机制募集到靶启动子，从而使转录开始。Pol II的最大亚基（称为RPB1或RPB205）是一个1840个氨基酸的蛋白质，包含一个C2H2型锌指和一个由几个七肽重复组成的C末端结构域。尽管Pol II功能需要所有12个亚基的协同作用，但最大亚基传递Pol II催化活性，并与第二大亚基一起形成Pol II酶的活性中心。此外，大亚基参与形成Pol II的DNA结合结构域，这是DNA模板转录所必需的沟槽。如果没有大亚基的正常功能，mRNA合成和随后的转录延伸就无法发生。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Pol II 抗体 (3WG2)

sc-21750
200 µg/ml
RMB2377.00

Pol II (3WG2): m-IgGκ BP-HRP 套装

sc-550731
200 µg Ab; 40 µg BP
RMB2662.00

How does PoI II (3WG2): sc-21750 generated? Could it recognize the YSPTSPS heptapeptide and dose it is without any phosphorylation? Thanks.

Asked by: Freca
Thank you for your question. This mouse monoclonal antibody was raised against conserved heptapeptide repeat on the largest subunit of RNA polymerase II. The exact epitope recognized by this monoclonal antibody has not yet been determined. We do not have any data suggesting the antibody is phospho-specific, and should be able to recognize the non-phosphorylated Pol II.
Answered by: Technical Support
Date published: 2018-11-21

What is the recommended storage condition for Pol II (3WG2): sc-21750?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good WB antibody in AGood WB antibody in A-431, U-205, NIH/3T3 and PC-12 whole cell lysates. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from Worked well in ChIP in human T47DWorked well in ChIP in human T47D, MCF7 cells. -SCBT Publication Review
Date published: 2014-04-17
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Pol II 抗体 (3WG2) is rated 5.0 out of 5 by 2.
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