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PML 抗体 (G-8): sc-377340

4.8(5)
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说明书
  • PML抗体(G-8)是小鼠单克隆IgG2b κ, 在5篇文献中引用,规格为200 µg/ml
  • 免疫human物种的PML的氨基酸157-394
  • 推荐用于 human 来源的all isoforms of PML WB, IP, IF, IHC(P) 和 ELISA检测
  • 可用于ChIP的TransCruz试剂(sc-377340 X, 200 µg/0.1 ml)
  • 看 PML (E-11): sc-377390,可查看有标记的 PML 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是PML Antibody (G-8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂,这些试剂现与PML Antibody (G-8) 打包提供(请参阅下面的订购信息)。
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PML蛋白是一种锌指转录因子,由于选择性剪接而表现为三种主要的转录产物。编码人类PML的基因位于染色体15q22。视黄酸受体α(RARα)基因的t(15;17)(q22;q11.2-q12)染色体易位几乎发生在所有急性早幼粒细胞白血病病例中,导致PML/RARα嵌合蛋白的表达。表达PML/RARα嵌合体的骨髓前体细胞不能分化,并因凋亡减少而表现出生长速度增加。PML/RARα通过招募核共抑制因子(N-CoR)-组蛋白去乙酰化酶复合物来转化骨髓前体细胞,该复合物对视黄酸依赖性骨髓分化至关重要。PML/RARα还招募DNA甲基转移酶,以诱导基因高甲基化和沉默,最终促进白血病的发生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PML 抗体 (G-8) 参考文献:

  1. 原粒细胞白血病(PML)蛋白在肿瘤抑制中的作用。  |  Rego, EM., et al. 2001. J Exp Med. 193: 521-29. PMID: 11181703
  2. 人类细胞周期蛋白 T1 通过与 PML 蛋白的直接相互作用被招募到核体中。  |  Marcello, A., et al. 2003. EMBO J. 22: 2156-66. PMID: 12727882
  3. SUMO-1对PML核蛋白的修饰可调节类风湿性关节炎滑膜纤维母细胞中Fas诱导的细胞凋亡。  |  Meinecke, I., et al. 2007. Proc Natl Acad Sci U S A. 104: 5073-8. PMID: 17360386
  4. PML 蛋白的表达调节 JCV 感染。  |  Gasparovic, ML., et al. 2009. Virology. 390: 279-88. PMID: 19523662
  5. 在端粒酶阳性的 MCF7 细胞中稳定表达早幼粒细胞白血病(PML)蛋白会导致端粒表型的替代性延长。  |  Yong, JW., et al. 2012. Genome Integr. 3: 5. PMID: 22925423
  6. 早幼粒细胞白血病(PML)蛋白在HSV-1感染的细胞内宿主免疫顺序调节中的不同时间作用。  |  Alandijany, T., et al. 2018. PLoS Pathog. 14: e1006769. PMID: 29309427
  7. 腺病毒感染诱导核体相关蛋白PML的核内分布重新排列。  |  Puvion-Dutilleul, F., et al. 1995. Exp Cell Res. 218: 9-16. PMID: 7737384
  8. 干扰素诱导正常细胞和急性早幼粒细胞白血病细胞中的PML蛋白。  |  Chelbi-Alix, MK., et al. 1995. Leukemia. 9: 2027-33. PMID: 8609713
  9. 早幼粒细胞白血病PML/RARα蛋白对分化的影响取决于PML蛋白二聚化和RARαDNA结合域的融合。  |  Grignani, F., et al. 1996. EMBO J. 15: 4949-58. PMID: 8890168
  10. 正常和肿瘤性人体组织中早幼粒细胞白血病(PML)蛋白的异质核表达。  |  Gambacorta, M., et al. 1996. Am J Pathol. 149: 2023-35. PMID: 8952536

订购信息

产品名称产品编号规格价格数量收藏夹

PML 抗体 (G-8)

sc-377340
200 µg/ml
CNY2422.00

PML (G-8): m-IgG Fc BP-HRP 套装

sc-538038
200 µg Ab; 10 µg BP
CNY2715.00

PML (G-8): m-IgGκ BP-HRP 套装

sc-535562
200 µg Ab; 40 µg BP
CNY2715.00

PML 抗体 (G-8) X

sc-377340 X
200 µg/0.1 ml
CNY2422.00

I am using PML (G-8): sc-377340 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by marilena from Everything ok!We have already tried the ab in both western and staining and we got great results. We are about to use it in Co-IP.
Date published: 2019-07-25
Rated 5 out of 5 by Randy McDonald from positive western blot in human tissueWe used this antibody for western blot in order to detect PML expression. We got positive bands shown up at the expected molecular weight.
Date published: 2017-01-27
Rated 5 out of 5 by ET from Superb immunoperoxidase nuclear and cytoplasmicSuperb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-04-30
Rated 5 out of 5 by SS from Published western blot analysis of PML usingPublished western blot analysis of PML using HEK cell lysates. -SCBT Publication Review
Date published: 2015-04-10
Rated 5 out of 5 by JM from Positive results obtained in WB in KPositive results obtained in WB in K-562 nuclear extract and human lymph node tissue extract. -SCBT QC
Date published: 2015-03-16
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PML 抗体 (G-8) is rated 4.8 out of 5 by 5.
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