Date published: 2025-9-8
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PML Antibody (PG-M3) - Immunofluorescence - Image 370906
PML 抗体 (PG-M3): sc-966. 对福尔马林固定的 A-431 细胞进行免疫荧光染色, 显示核体定位.
PML 抗体 (PG-M3): sc-966. 对福尔马林固定的 A-431 细胞进行免疫荧光染色, 显示核体定位.

PML 抗体 (PG-M3): sc-966

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说明书
  • PML抗体(PG-M3)是小鼠单克隆IgG1 κ, 在709篇文献中引用，规格为200 µg/ml
  • 抗原表位对应的氨基酸序列37-51位于human物种的PML的N-terminal的附近
  • PML 抗体 (PG-M3) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 all isoforms of PML
  • 抗PML抗体（PG-M3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-966 X, 200 µg/0.1 ml)
  • 关于如何获取PML (PG-M3): sc-966的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 PML 抗体 (PG-M3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PML 抗体 (PG-M3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PML抗体（PG-M3）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）检测人类来源的PML蛋白。PML (PG-M3) 单克隆抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。PML是一种锌指转录因子，对于调节细胞分化与凋亡相关的基因表达至关重要。在急性早幼粒细胞白血病中，PML在这些过程中的作用尤为重要，因为PML基因与视黄酸受体α（RARα）基因发生染色体易位，形成PML/RARα嵌合蛋白。这种异常蛋白会破坏正常的骨髓分化，因为它会招募核共抑制剂-组蛋白去乙酰化酶复合物，而后者对于视黄酸依赖性分化至关重要。此外，这种异常蛋白还会诱导基因高甲基化，导致关键基因沉默并促进白血病发生。PML能够影响这些途径，这凸显了PML在正常细胞功能和疾病状态中的重要性，使PML（PG-M3）抗体成为研究人员研究这些机制的有用工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PML 抗体 (PG-M3) 参考文献:

  1. 一种致癌转录因子对目标启动子的甲基转移酶招募和DNA超甲基化作用。  |  Di Croce, L., et al. 2002. Science. 295: 1079-82. PMID: 11834837
  2. 鉴定所有急性早幼粒细胞白血病（APL）患者视黄酸受体-α（RAR-α）基因座的 DNA 重排，并绘制 RAR-α 第二内含子中的 APL 断点图。意大利合作研究小组 'GIMEMA'。  |  Diverio, D., et al. 1992. Blood. 79: 3331-6. PMID: 1317727
  3. 急性早幼粒细胞白血病 myl/RAR alpha cDNA 的结构和起源及其视黄醇结合和转录激活特性的鉴定。  |  Pandolfi, PP., et al. 1991. Oncogene. 6: 1285-92. PMID: 1650447
  4. 人类急性早幼粒细胞白血病的染色体易位 t(15;17) 使 RAR alpha 与一种新型推定转录因子 PML 融合。  |  Kakizuka, A., et al. 1991. Cell. 66: 663-74. PMID: 1652368
  5. 急性早幼粒细胞白血病中被 t（15;17）基因破坏的锌指基因的特征。  |  Goddard, AD., et al. 1991. Science. 254: 1371-4. PMID: 1720570
  6. 急性早幼粒细胞白血病的 t(15;17)易位将视黄酸受体 alpha 基因融合到一个新的转录位点上。  |  de Thé, H., et al. 1990. Nature. 347: 558-61. PMID: 2170850
  7. 17 号染色体上急性早幼粒细胞白血病断点群区的分子分析。  |  Borrow, J., et al. 1990. Science. 249: 1577-80. PMID: 2218500
  8. 早幼粒细胞白血病（PML）蛋白在调节细胞粘附、形态、增殖和迁移方面发挥着重要作用。  |  Tang, MK., et al. 2013. PLoS One. 8: e59477. PMID: 23555679
  9. PML蛋白组织异染色质域，通过ATRX/DAXX调节组蛋白H3.3的沉积。  |  Delbarre, E., et al. 2017. Genome Res. 27: 913-921. PMID: 28341773
  10. 磷酸化SIM和PML蛋白的exon8b调节阿霉素诱导的rDNA-PML隔室的形成。  |  Hornofova, T., et al. 2022. DNA Repair (Amst). 114: 103319. PMID: 35325646
  11. 急性早幼粒细胞白血病特异性 PML-RAR alpha 融合蛋白可抑制骨髓前体细胞的分化并促进其存活。  |  Grignani, F., et al. 1993. Cell. 74: 423-31. PMID: 8394219
  12. 视黄酸受体-α融合蛋白在早幼粒细胞白血病中招募组蛋白去乙酰化酶。  |  Grignani, F., et al. 1998. Nature. 391: 815-8. PMID: 9486655

订购信息

产品名称产品编号规格价格数量收藏夹

PML 抗体 (PG-M3)

sc-966
200 µg/ml
RMB2377.00

PML (PG-M3): m-IgG Fc BP-HRP 套装

sc-528122
200 µg Ab; 10 µg BP
RMB2662.00

PML (PG-M3): m-IgGκ BP-HRP 套装

sc-520425
200 µg Ab, 40 µg BP
RMB2662.00

PML (PG-M3): m-IgG1 BP-HRP 套装

sc-542774
200 µg Ab; 20 µg BP
RMB2662.00

PML 抗体 (PG-M3) X

sc-966 X
200 µg/0.1 ml
RMB2377.00

PML 抗体 (PG-M3) AC

sc-966 AC
500 µg/ml, 25% agarose
RMB3129.00

PML 抗体 (PG-M3) HRP

sc-966 HRP
200 µg/ml
RMB2377.00

PML 抗体 (PG-M3) FITC

sc-966 FITC
200 µg/ml
RMB2482.00

PML 抗体 (PG-M3) PE

sc-966 PE
200 µg/ml
RMB2580.00

PML 抗体 (PG-M3) Alexa Fluor® 488

sc-966 AF488
200 µg/ml
RMB2685.00

PML 抗体 (PG-M3) Alexa Fluor® 546

sc-966 AF546
200 µg/ml
RMB2685.00

PML 抗体 (PG-M3) Alexa Fluor® 594

sc-966 AF594
200 µg/ml
RMB2685.00

PML 抗体 (PG-M3) Alexa Fluor® 647

sc-966 AF647
200 µg/ml
RMB2685.00

PML 抗体 (PG-M3) Alexa Fluor® 680

sc-966 AF680
200 µg/ml
RMB2685.00

PML 抗体 (PG-M3) Alexa Fluor® 790

sc-966 AF790
200 µg/ml
RMB2685.00

PML 抗体 (PG-M3) TRITC

sc-966 TRITC
200 µg/ml
RMB2407.00

请问可以申请试用装吗

Asked by: 陈 永俊
感谢您的提问。试用装的申请原则是需要购买一抗的正装，可以随单申请三个不同货号，不同目的蛋白的试用装。 Thank you for your question. The principle of application for trial packaging is to purchase the formal packaging of the primary antibody. You can apply for three trial packaging with different product numbers and different target proteins.
Answered by: Zhanglong
Date published: 2024-10-29

Can I get an epitope sequence of sc966 anti-PML antibody? I would like to check if I could use it to blot mouse protein on IF. Thank you! Won

Asked by: Wonkyun
Thank you for your question. This antibody was raised against an epitope corresponding to amino acids 37-51 mapping near the N-terminus of PML of human origin. This antibody has not yet been tested in mouse and so that is not a recommended species at this time.
Answered by: Tech Service
Date published: 2020-02-10

For molecular weight of PML, it displayed 55-90KD, others showed 90-132KD. So, what is the difference?

Asked by: dafdafda
Thank you for your question. The PML protein has to main isoforms which run around 78 and 92kDa. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service
Date published: 2023-05-15

How do I obtain PG M3 antibody to India?

Asked by: Merin
Thank you for your question. Please contact one of our Distributors in India to obtain this antibody.
Answered by: Technical Support
Date published: 2019-01-06

Does this antibody also bind mouse PML?

Asked by: Timo
Thank you for your question. PML Antibody (PG-M3): sc-966 is only recommended for detection of all isoforms of PML of human origin by WB, IP, IF and IHC(P).
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

Ho visto che è possibile provare questo ab (sc-966) con una trial size. Come posso procedere per provarlo?

Asked by: Marina55
Molte grazie per la sua domanda. Il prodotto sc-966 é fuori catalogo e sostituito con il mSin3B (H-4): sc-13145. Questo nuovo anticorpo monoclonale é piú specifico, fornisce un segnale piú forte e infine elimina la conosciuta variabilitá tra lotti degli anticorpi policlonali. Per ricevere il campione gratuito di mSin3B (H-4): sc-13145 la prego di contattare il nostro servizio tecnico tramite telefono, email o live chat. Thank you for your question. The mSin3B (A-20) has been discontinued and replaced by mSin3B (H-4): sc-13145. This new monoclonal antibody is more specific, provides a stronger signal and eliminates lot to lot variation characteristic of most, if not all, polyclonal antibodies. If you have an interest in testing a free 10 µg sample of mSin3B (H-4): sc-13145 please contact Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

hi, who can i source this from in Australasia please? We cannot buy from Thermofisher NZD who were our previous supplier. Please advise urgently. Many thanks

Asked by: kate marson
Thank you for the question. The antibody is currently in stock. Please send an email with your full shipping & billing address by email to customercare @ scbt.com. The colleagues from our Customer Service dept. will be happy to contact you.
Answered by: Technical Support Europe
Date published: 2017-12-27

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one of our Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16
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Rated 5 out of 5 by from Great featuresI bought this a month ago and i just wanna say that i love it
Date published: 2023-10-12
Rated 5 out of 5 by from great featuresI have been using this product for 1 month and am very happy with its effectiveness
Date published: 2023-09-28
Rated 5 out of 5 by from Very clear signal!We applied Anti-PML antibody (PG-M3) in immunofluorescence, to visualize PML bodies in U2OS cell line. Antibody at 1:1000 dilution in Goat serum blocking solution (1mg/ml BSA, 3% goat serum, 0,1% Triton X-100, 1mM EDTA ph 8.0) and O.N incubation produced a strong specific signal with no background. solution (1mg/ml BSA, 3% goat serum, 0,1% Triton X-100, 1mM EDTA ph 8.0).
Date published: 2022-06-03
Rated 5 out of 5 by from Good mAb for IFAAs noted in a lot of papers, this monoclonal antibody works well in IFA. Although This mAb has back-ground signal, the positive signal of PML is strong to obtain good picture. (HeLa cell, 1:100 dilution in 1% BSA/PBS, 4C overnight)
Date published: 2020-11-24
Rated 4 out of 5 by from RecommendedWorks well by Western blotting and immunofluorescence microscopy.
Date published: 2020-05-20
Rated 5 out of 5 by from It works great!I bought this antibody one month ago. It does work in my IF analysis.
Date published: 2019-01-10
Rated 5 out of 5 by from Antibody is AWESOMEThis is the first time i tried the HRP tagged antibody and the result was unexpected and also it saved my time. With the very less concentration than i used to use before gave me a clear result. I will try to purchase HRP tagged antibodies for my work from now also i have recommended these antibodies to my collegues too.
Date published: 2018-07-13
Rated 5 out of 5 by from Great for IPI have used the PML antibody for IP and it works very well. I recommend this antibody for RIP (RNA-IP) and ChIP applications as well.
Date published: 2018-02-23
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PML抗体 (PG-M3) is rated 4.8 out of 5 by 18.
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