Date published: 2025-9-8

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Plectin 抗体 (7A8): sc-58835

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说明书
  • Plectin抗体(7A8)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 针对Plectin纯化的rat来源胶质瘤C6细胞
  • 推荐用于 mouse, rat 和 human 来源的an epitope in the rod domain of Plectin WB, IP 和 IF检测
  • 看 Plectin (10F6): sc-33649,可查看有标记的 Plectin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Plectin Antibody (7A8) for WB applications. 的首选辅助检测试剂,这些试剂现与Plectin Antibody (7A8) 打包提供(请参阅下面的订购信息)。

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    関連項目

    Plectin是一种丰富的细胞骨架蛋白,参与细胞质稳定。Plectin已被证明可将中间丝交联到微管和微丝上,并将中间丝锚定到质膜和核膜上。Plectin与Lamin B和波形蛋白结合,这种结合受多种蛋白激酶的调节。PKA或PKC的磷酸化导致与Lamin B的结合减少,而PKA的磷酸化增强了plectin-vimentin的相互作用。Plectin也是p34cdc2激酶的底物。已知存在plectin的几种选择性剪接异构体。已知人类凝血素突变可引起伴肌营养不良的单纯型大疱性表皮松解症(EBS-MD)。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Plectin 抗体 (7A8) 参考文献:

    1. 确定小鼠肌母细胞和角质形成细胞中 plectin 的机械特性。  |  Bonakdar, N., et al. 2015. Exp Cell Res. 331: 331-7. PMID: 25447312
    2. 杆状结构域对 plectin 维护组织完整性的功能并不重要。  |  Ketema, M., et al. 2015. Mol Biol Cell. 26: 2402-17. PMID: 25971800
    3. Plectin 通过介导波形蛋白和肌动蛋白网络之间的串扰,加强了血管的完整性。  |  Osmanagic-Myers, S., et al. 2015. J Cell Sci. 128: 4138-50. PMID: 26519478
    4. 用于胰腺癌成像的 Plectin-1 靶向双模式纳米粒子  |  Chen, X., et al. 2018. EBioMedicine. 30: 129-137. PMID: 29574092
    5. 进行性家族性肝内胆汁淤积症中的 Plectin 基因突变  |  Wu, SH., et al. 2019. Hepatology. 70: 2221-2224. PMID: 31269534
    6. 表观遗传学在骨关节炎发病机制和进展中的作用 AK Sorial 等:骨关节炎易感基因座映射到 plectin 基因 PLEC 的多组织表观遗传学分析。  |  Malemud, CJ. 2020. Osteoarthritis Cartilage. 28: 1401-1402. PMID: 32891768
    7. 整合素α6β4需要plectin和vimentin来实现粘附复合物的分布和侵袭性生长。  |  Qi, L., et al. 2022. J Cell Sci. 135: PMID: 34897465
    8. 成纤维细胞中 Plectin 的缺失会改变中间丝和细胞器的形态、迁移和粘附。  |  Zrelski, MM., et al. 2024. J Invest Dermatol. 144: 547-562.e9. PMID: 37716646
    9. 基于 DOTA 的 Plectin-1 靶向对比剂可检测人体组织中的胰腺癌。  |  Gazzi, T., et al. 2024. Angew Chem Int Ed Engl. 63: e202318485. PMID: 38608197
    10. 癌细胞外渗需要iplectin介导的MT1-MMP在内陷嵴的传递。  |  Grafinger, OR., et al. 2024. Br J Cancer. 131: 931-943. PMID: 38969866

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Plectin 抗体 (7A8)

    sc-58835
    200 µg/ml
    RMB2377.00

    Plectin (7A8): m-IgG Fc BP-HRP 套装

    sc-539101
    200 µg Ab; 10 µg BP
    RMB2662.00

    Plectin (7A8): m-IgG1 BP-HRP 套装

    sc-541259
    200 µg Ab; 20 µg BP
    RMB2662.00

    How much sample do you recommend loading on the gel for Western blot detection using Plectin (7A8): sc-58835?

    Asked by: jenniferc
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 4 out of 5 by from Band detected at expected mw in rat adrenalBand detected at expected mw in rat adrenal tissue extract. -SCBT QC
    Date published: 2015-03-19
    Rated 4 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using rat brain tissue extract. -SCBT QC
    Date published: 2014-10-14
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    Plectin 抗体 (7A8) is rated 4.0 out of 5 by 2.
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