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Plectin Antibody (10F6) - Immunofluorescence - Image 12119
Plectin 抗体 (10F6): sc-33649. 甲醇固定的 A549 细胞免疫荧光染色, 显示细胞骨架定位.
Plectin 抗体 (10F6): sc-33649. 甲醇固定的 A549 细胞免疫荧光染色, 显示细胞骨架定位.

Plectin 抗体 (10F6): sc-33649

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寫評論提問

说明书
  • Plectin 抗体 10F6 是小鼠单克隆 IgG1 κ，Plectin抗体, 在30篇文献中引用，规格为200 µg/ml
  • 针对纯化的rat起源的胶质瘤C6细胞Plectin
  • Plectin 抗体 (10F6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Plectin
  • 抗Plectin抗体（10F6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Plectin (10F6): sc-33649的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Plectin 抗体 (10F6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Plectin 抗体 (10F6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Plectin 抗体（10F6）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 Plectin 蛋白。抗Plectin抗体（10F6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Plectin是一种重要的细胞骨架蛋白，通过将中间丝与微管和微丝交联，从而稳定细胞质，在维持细胞完整性方面发挥着重要作用。Plectin能够将中间丝锚定在质膜和核膜上，这对于细胞结构和功能至关重要。Plectin与Lamin B和vimentin等关键蛋白相互作用，这些相互作用由各种蛋白激酶调节。例如，蛋白激酶A或蛋白激酶C的磷酸化会降低与Lamin B的结合亲和力，而蛋白激酶A的磷酸化则会增强与vimentin的相互作用。Plectin是p34cdc2激酶的底物，这表明它参与细胞周期的调节。Plectin有多种可选择的剪接异构体，人类Plectin基因的突变与表皮松解性大疱性单纯性肌营养不良（EBS-MD）有关，这表明它在结构和病理学方面都很重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Plectin 抗体 (10F6) 参考文献:

  1. Plectin。  |  Rezniczek, GA., et al. 2004. Methods Cell Biol. 78: 721-55. PMID: 15646637
  2. 蛋白激酶 A 和蛋白激酶 C 调节的 plectin 与层粘连蛋白 B 和波形蛋白的相互作用。  |  Foisner, R., et al. 1991. Proc Natl Acad Sci U S A. 88: 3812-6. PMID: 2023931
  3. 与 Plectin 相关的肩胛骨肌病，伴有治疗反应性肌萎缩综合征。  |  Argente-Escrig, H., et al. 2021. Neuropathol Appl Neurobiol. 47: 352-356. PMID: 32757300
  4. Plectin 连接具有机械敏感性，是三维细胞迁移的核活塞机制所必需的。  |  Marks, PC., et al. 2022. Mol Biol Cell. 33: ar104. PMID: 35857713
  5. Plectin 通过调节 Rous 肉瘤癌基因的活性促进 B16 小鼠黑色素瘤细胞形成肿瘤。  |  Mizuta, K., et al. 2022. BMC Cancer. 22: 936. PMID: 36038818
  6. 确定 plectin 为 p34cdc2 激酶的底物并绘制单一磷酸化位点图。  |  Malecz, N., et al. 1996. J Biol Chem. 271: 8203-8. PMID: 8626512
  7. 人类 plectin：基因组织, 序列分析和染色体定位（8q24）。  |  Liu, CG., et al. 1996. Proc Natl Acad Sci U S A. 93: 4278-83. PMID: 8633055
  8. Plectin 转录本多样性：具有不同第一编码外显子和无杆异构体的变体的鉴定和组织分布。  |  Elliott, CE., et al. 1997. Genomics. 42: 115-25. PMID: 9177781
  9. plakin家族：细胞骨架结构的多功能组织者。  |  Ruhrberg, C. and Watt, FM. 1997. Curr Opin Genet Dev. 7: 392-7. PMID: 9229116
  10. 对 plectin 的靶向灭活揭示了其在维持皮肤, 肌肉和心脏细胞结构完整性方面的重要功能。  |  Andrä, K., et al. 1997. Genes Dev. 11: 3143-56. PMID: 9389647
  11. Plectin 在细胞骨架组织和动力学中的作用  |  Wiche, G. 1998. J Cell Sci. 111 (Pt 17): 2477-86. PMID: 9701547
  12. 人中枢神经系统中的 Plectin：在 pia/glia 和内皮细胞/glia 界面的主要表达。  |  Lie, AA., et al. 1998. Acta Neuropathol. 96: 215-21. PMID: 9754951

订购信息

产品名称产品编号规格价格数量收藏夹

Plectin 抗体 (10F6)

sc-33649
200 µg/ml
RMB2377.00

Plectin (10F6): m-IgG Fc BP-HRP 套装

sc-526696
200 µg Ab; 10 µg BP
RMB2662.00

Plectin (10F6): m-IgG1 BP-HRP 套装

sc-532069
200 µg Ab; 20 µg BP
RMB2662.00

Plectin 抗体 (10F6) AC

sc-33649 AC
500 µg/ml, 25% agarose
RMB3129.00

Plectin 抗体 (10F6) HRP

sc-33649 HRP
200 µg/ml
RMB2377.00

Plectin 抗体 (10F6) FITC

sc-33649 FITC
200 µg/ml
RMB2482.00

Plectin 抗体 (10F6) PE

sc-33649 PE
200 µg/ml
RMB2580.00

Plectin 抗体 (10F6) Alexa Fluor® 488

sc-33649 AF488
200 µg/ml
RMB2685.00

Plectin 抗体 (10F6) Alexa Fluor® 546

sc-33649 AF546
200 µg/ml
RMB2685.00

Plectin 抗体 (10F6) Alexa Fluor® 594

sc-33649 AF594
200 µg/ml
RMB2685.00

Plectin 抗体 (10F6) Alexa Fluor® 647

sc-33649 AF647
200 µg/ml
RMB2685.00

Plectin 抗体 (10F6) Alexa Fluor® 680

sc-33649 AF680
200 µg/ml
RMB2685.00

Plectin 抗体 (10F6) Alexa Fluor® 790

sc-33649 AF790
200 µg/ml
RMB2685.00

Could you please inform me which epitope does this antibody recognize?

Asked by: Nsyja
This is a mouse monoclonal antibody raised against purified glioma C6 cell Plectin of rat origin. The exact epitope recognized by this monoclonal antibody has not been determined. However, the following reference may be helpful, PMID: 1704007.
Answered by: Technical Support
Date published: 2018-12-14

Is it possible to use this antibody for IF or IHC on mouse tissue samples?

Asked by: Anna99
Yes, this antibody comes conjugated to HRP (sc-33649 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-33649 PE), fluorescein (sc-33649 FITC), Alexa Fluor® 488 (sc-33649 AF488) or Alexa Fluor® 647 (sc-33649 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Service 11
Date published: 2017-01-13

What is the best secondary to use with this mouse antibody?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-13

Hi, I am attempting to use this antibody (plectin 10F6: sc-33649) on formalin fixed,paraffin embedded archival ovarian cancer samples. Can you please give me some recommendations for antigen retrieval please, have tried microwave 15 mins low and high pH

Asked by: Weaver
Thank you for your question. We have not yet tested this antibody, sc-33649: Plectin (1046) using formalin-fixed paraffin-embedded tissue sections so we do not have any specific recommendations for antigen retrieval using this antibody at this time. This antibody is recommended for IF, or immunofluorescent staining of fresh or frozen tissue sections or cells fixed in acetone or methanol. In general, we recommend using a sodium citrate buffer, pH 6 using a heat treatment in a microwave for antigen retrieval.
Answered by: Technical Support
Date published: 2016-09-15
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Rated 5 out of 5 by from works in WBThis antibody worked showed specific bands in WB experiments with tongue squamous cell carcinoma CAl27, in line with the mass spectrometry results.
Date published: 2023-01-12
Rated 5 out of 5 by from good products and it Works wellthis antibody works well for western blot with clean background and specific band in pig skeletal muscle.
Date published: 2018-06-19
Rated 5 out of 5 by from Works wellWorked well for immunostaining and Western blot applications of primary astrocyte samples
Date published: 2018-01-06
Rated 5 out of 5 by from Great productAll applications show outstanding results; best plectin antibody
Date published: 2017-01-19
Rated 5 out of 5 by from Good stainingThis monoclonal Plectin antibody shows good results for IF, and strong signal in WB.
Date published: 2017-01-11
Rated 3 out of 5 by from this antibody does stain expected structuresthis antibody does stain expected structures but does so very weakly even at 1:100 dilution.
Date published: 2016-06-24
Rated 5 out of 5 by from Good antibody for westernGood antibody for western, clean background and specific band in rat brain tissue extract. - SCBT QC
Date published: 2015-06-24
Rated 5 out of 5 by from Published data for IPPublished data for IP, IF and WB in human platelets. -SCBT Publication Review
Date published: 2015-06-20
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Plectin 抗体 (10F6) is rated 4.8 out of 5 by 11.
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