Date published: 2025-9-7

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Pleckstrin 抗体 (227.1): sc-100813

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说明书
  • Pleckstrin抗体(227.1)是小鼠单克隆IgG2a (kappa轻链),规格为100 µg/ml
  • 免疫human物种的重组Pleckstrin
  • 推荐用于 human 来源的Pleckstrin WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgGκ BP-HRP 是 Pleckstrin 抗体 (227.1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Pleckstrin 抗体 (227.1) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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    激活血小板中的蛋白激酶C(PKC)会立即导致血小板中主要的PKC底物pleckstrin(以前称为40K或P47)的磷酸化。Pleckstrin包含一个Ca2+结合的"EF-hand"结构,并在Ser 113和Ser 117具有PKC磷酸化位点。Pleckstrin的N端和C端包含两个pleckstrin同源结构域(PH),它们介导蛋白质-蛋白质和蛋白质-脂质的相互作用。Pleckstrin在人类中性粒细胞中高表达。中性粒细胞经炎症刺激处理后,Pleckstrin迅速磷酸化,可能是由非常规PKC异构体delta或zeta所介导的,这两种异构体在人类中性粒细胞中表达。非常规PKC异构体介导的磷酸化诱导Pleckstrin发生构象变化,促进其与膜和/或细胞骨架的相互作用,从而将PH结构域识别的蛋白质或脂质靶向到它们可以有助于杀菌反应的位点。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Pleckstrin 抗体 (227.1)

    sc-100813
    100 µg/ml
    $333.00

    Pleckstrin (227.1): m-IgGκ BP-HRP 套装

    sc-521184
    100 µg Ab, 40 µg BP
    $354.00

    Hi, I would like to use a pleckstrin antibody for immunofluorescence/IHC in mouse embryonic tissue. Which antibody clone would you recommend?

    Asked by: drts
    Thank you for your question. We do not currently offer a Pleckstrin antibody that is recommended for IF or IHC on mouse tissue. Please check back for updates! This antibody has been validated for IHC(P) in human samples, but we do not yet have data or product citations in mouse samples.
    Answered by: Technical Support
    Date published: 2019-09-10

    Hi, can I use this to detect mouse Pleckstrin?

    Asked by: drts
    Thank you for your question. This antibody, sc-100813, has not been validated on mouse samples and can not be recommended or warrantied for that use. We do offer a mouse reactive Pleckstrin antibody: sc-136042. You can find it here: https://www.scbt.com/p/pleckstrin-antibody-25
    Answered by: Tech Service
    Date published: 2019-09-10

    Can sc-100813: Pleckstrin (227.1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Trav11
    Thank you for your question. Yes, sc-100813: Pleckstrin (227.1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human spleen tissue. -SCBT QC
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    Pleckstrin 抗体 (227.1) is rated 5.0 out of 5 by 2.
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