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PKR 抗体 (FJ-6): sc-100378

4.8(9)
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说明书
  • PKR抗体(FJ-6)是小鼠单克隆IgG2a κ, 在15篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组PKR
  • 推荐用于 human 来源的PKR WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 PKR (B-10): sc-6282,可查看有标记的 PKR 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成PKR 抗体 (FJ-6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

PKR抗体(FJ-6)是一种小鼠单克隆IgG2a抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人类样本中的PKR。PKR是一种干扰素诱导的、RNA依赖的蛋白丝氨酸/苏氨酸激酶,通过磷酸化蛋白质合成起始因子eIF-2的α亚基,在细胞对病毒感染的反应中发挥关键作用。这种对丝氨酸-51的磷酸化反应会导致翻译抑制,有效阻止病毒复制,并使宿主细胞产生免疫反应。PKR的结构由不同的功能域组成,丝氨酸/苏氨酸激酶催化域位于羧基末端,RNA结合域位于氨基末端。这种结构使PKR能够对各种刺激做出反应,包括病毒感染的特征--双链RNA。PKR的酶活性通过多种调节机制进行微调,包括对干扰素的转录调控、翻译水平的自调节机制以及RNA介导的自磷酸化等翻译后修饰。抗PKR抗体(FJ-6)是研究免疫反应和病毒发病机理的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

PKR 抗体 (FJ-6)

sc-100378
100 µg/ml
RMB2504.00

anti-PKR(FJ-6): sc-100378はmouseで検出できるでしょうか?他の動物種との交差性を教えてください。

Asked by: ウィルス学
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Can sc-100378: PKR (FJ-6) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, sc-100378: PKR (FJ-6) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from 良いです。この抗体は、内在性タンパク質を容易に検出できます。一次抗体は生理食塩水(PBS)で希釈しました。良いです。
Date published: 2020-10-20
Rated 5 out of 5 by from Great antibody for western analysisThis antibody produced a strong signal with little background for the western analysis when used at 1:500 dilution.
Date published: 2019-11-25
Rated 5 out of 5 by from Great monoclonal antibodyRobust staining by IHCP and IF. Strong and specific band observed with PKR Antibody (FJ-6)
Date published: 2017-02-08
Rated 5 out of 5 by from Publishable WB dataPublishable WB data, successful detection of PKR in human lung epithelial cells (A549). -SCBT Publication Review
Date published: 2015-04-10
Rated 4 out of 5 by from Produced robust immunoperoxidase nuclearProduced robust immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-03-26
Rated 5 out of 5 by from Publishable Western blot results using humanPublishable Western blot results using human A549 and PBMCs. -SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by from WB Published with this antibody using human Huh7WB Published with this antibody using human Huh7.5.1 cells for immunoblot analysis -SCBT Publication Review
Date published: 2015-02-12
Rated 5 out of 5 by from Precise band obtained in WB using APrecise band obtained in WB using A-431 whole cell lysate. -SCBT QC
Date published: 2015-02-08
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PKR 抗体 (FJ-6) is rated 4.8 out of 5 by 9.
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