Date published: 2025-9-6

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PKC theta 抗体 (E-7): sc-1680

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说明书
  • PKC theta 抗体 E-7 是小鼠单克隆 IgG2a κ,PKC theta抗体, 在24篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的PKC †的C-terminus的氨基酸690-707之间
  • PKC theta 抗体 (E-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PKC θ
  • 抗PKC theta抗体(E-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PKC theta (E-7): sc-1680免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是PKC theta Antibody (E-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PKC theta Antibody (E-7) 打包提供(请参阅下面的订购信息)。

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関連項目

PKC θ 抗体(E-7)是一种小鼠单克隆 IgG2a kappa 轻链抗体,专门针对蛋白激酶 C theta(PRKCQ)蛋白。PKC θ 单克隆抗体(E-7)可识别小鼠 PKC θ C 末端 690-707 氨基酸之间的精确表位,可用于检测多种物种,包括小鼠、大鼠和人类。PKC θ,也称为蛋白激酶 C θ,是一种参与T细胞活化和免疫反应调节的关键酶,因此成为免疫学和治疗研究的重要研究对象。PKC θ在细胞生长、分化、基因表达、激素分泌和膜功能等细胞过程中发挥着关键调节作用,凸显了PKC θ在维持细胞稳态和信号通路中的重要性。PKC θ 单克隆抗体(E-7)用途广泛,在蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)中均表现出色,有助于在各种实验条件下进行综合分析。此外,PKC θ (E-7) 抗体有非结合型和多种结合型,包括辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和几种 Alexa Fluor® 结合物,可满足各种研究需求。PKC θ 研究有助于深入了解免疫调节的机制,并确定自身免疫性疾病和癌症的潜在治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC theta 抗体 (E-7) 参考文献:

  1. 哺乳动物的 TOR 控制着作用于 nPKCdelta 和 nPKCepsilon 的两种激酶途径之一。  |  Parekh, D., et al. 1999. J Biol Chem. 274: 34758-64. PMID: 10574945
  2. 经 H2O2 处理的细胞中蛋白激酶 C delta 的磷酸化位点及其在体外被酪氨酸激酶激活的情况。  |  Konishi, H., et al. 2001. Proc Natl Acad Sci U S A. 98: 6587-92. PMID: 11381116
  3. 原代小鼠淋巴细胞中 TCR 诱导的 PKCtheta 磷酸化的特征。  |  Thebault, S. and Ochoa-Garay, J. 2004. Mol Immunol. 40: 931-42. PMID: 14725789
  4. 蛋白激酶 C 家族的新成员 nPKC theta 主要在骨骼肌中表达。  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  5. 膜磷脂对多功能蛋白激酶的钙依赖性激活。  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  6. 肌醇磷脂的周转和信号转导  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  7. 蛋白激酶 C 在细胞表面信号转导和肿瘤促进中的作用。  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  8. 蛋白激酶 C 可能是促进肿瘤生长的植物醇酯的受体蛋白。  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  9. 促进肿瘤生长的植物醇酯直接激活钙激活的磷脂依赖性蛋白激酶。  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
  10. 在 H2O2 作用下通过酪氨酸磷酸化激活蛋白激酶 C。  |  Konishi, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 11233-7. PMID: 9326592

订购信息

产品名称产品编号规格价格数量收藏夹

PKC theta 抗体 (E-7)

sc-1680
200 µg/ml
RMB2377.00

PKC theta (E-7): m-IgG Fc BP-HRP 套装

sc-525428
200 µg Ab; 10 µg BP
RMB2662.00

PKC theta (E-7): m-IgG2a BP-HRP 套装

sc-546251
200 µg Ab; 10 µg BP
RMB2662.00

PKC theta 抗体 (E-7) AC

sc-1680 AC
500 µg/ml, 25% agarose
RMB3129.00

PKC theta 抗体 (E-7) HRP

sc-1680 HRP
200 µg/ml
RMB2377.00

PKC theta 抗体 (E-7) FITC

sc-1680 FITC
200 µg/ml
RMB2482.00

PKC theta 抗体 (E-7) PE

sc-1680 PE
200 µg/ml
RMB2580.00

PKC theta 抗体 (E-7) Alexa Fluor® 488

sc-1680 AF488
200 µg/ml
RMB2685.00

PKC theta 抗体 (E-7) Alexa Fluor® 546

sc-1680 AF546
200 µg/ml
RMB2685.00

PKC theta 抗体 (E-7) Alexa Fluor® 594

sc-1680 AF594
200 µg/ml
RMB2685.00

PKC theta 抗体 (E-7) Alexa Fluor® 647

sc-1680 AF647
200 µg/ml
RMB2685.00

PKC theta 抗体 (E-7) Alexa Fluor® 680

sc-1680 AF680
200 µg/ml
RMB2685.00

PKC theta 抗体 (E-7) Alexa Fluor® 790

sc-1680 AF790
200 µg/ml
RMB2685.00

PKC theta (E-7) 中和勝肽

sc-1680 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-1680 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from good antibodyantibody works good in rat muscle, dilution 1:1000. More than one band was detected.
Date published: 2018-09-05
Rated 5 out of 5 by from GoodI got the antibody about several days ago,and I use it for my WB assay. But I am not very satisfied with the results. It's just okay
Date published: 2017-07-21
Rated 5 out of 5 by from 条带很清晰,很好用该抗体实惠又好用,已经在santa定了数十支抗体了!一直使用该品牌抗体,非常信任,支持!希望越来越好!
Date published: 2017-07-20
Rated 3 out of 5 by from not sure good or not, need repeat.I run two batch of sample on it, it is only 85KD band, there is no 55KD/50KD band. and one batch of my sample is good, another batch of my sample lost half of the band density, I do not know the reason, I will repeat once before make the conclusion.
Date published: 2017-05-22
Rated 5 out of 5 by from Good resultsThis antibody gave good results with single band in WB on human PBMC.
Date published: 2017-05-20
Rated 5 out of 5 by from Good for WBThis antibody will produce clear and specific bands of the correct weight in your WB. It will also perform well in IF. This product has been used for publishing 15 times.
Date published: 2017-02-02
Rated 5 out of 5 by from Good WB data with Membrane proteinI used the antibody 1:500 with Mouse liver Membrane protein and I got a very clear,signal bands
Date published: 2017-01-13
Rated 5 out of 5 by from Good WB publishable data gathered with CacoGood WB publishable data gathered with Caco-2, Jurkat E6.1 T cells and Raji B cells. -SCBT Publication Review
Date published: 2015-03-09
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PKC theta 抗体 (E-7) is rated 4.5 out of 5 by 11.
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