Date published: 2025-9-6
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PKC gamma Antibody (C-4) - Western Blotting - Image 352012
PKC γ 抗体 (C-4): sc-166385. 小鼠脑 (A) 和大鼠脑 (B) 组织提取物中 PKC γ 表达的 Western 印迹分析.
PKC γ 抗体 (C-4): sc-166385. 小鼠脑 (A) 和大鼠脑 (B) 组织提取物中 PKC γ 表达的 Western 印迹分析.

PKC gamma 抗体 (C-4): sc-166385

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说明书
  • PKC gamma 抗体 C-4 是小鼠单克隆 IgG2a κ，PKC gamma抗体, 在18篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的PKC γ的C-terminus的氨基酸673-697之间
  • PKC gamma 抗体 (C-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PKC γ
  • 抗PKC gamma抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PKC gamma (C-4): sc-166385的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 PKC gamma 抗体 (C-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PKC gamma 抗体 (C-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PKC 抗体包括 PKC α, PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ 和 PKC ν

PKC γ抗体（C-4）是一种IgG2a κ小鼠单克隆PKC γ抗体（也称为PKC γ抗体、蛋白激酶C γ抗体、PRKCG抗体或PKCG抗体），它通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的PKC γ蛋白。PKC γ抗体（C-4）既可作为非共轭的抗PKC γ抗体形式，也可作为多种共轭的抗PKC γ抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。蛋白激酶C（PKC）家族成员在多种细胞功能中发挥着关键的调节作用，包括细胞生长和分化、基因表达、激素分泌和膜功能。蛋白激酶C最初被鉴定为丝氨酸/苏氨酸蛋白激酶，其活性依赖于钙和磷脂。二酰甘油（DAG）和促肿瘤佛波酯与蛋白激酶C结合并激活它。蛋白激酶C可分为至少两大类，包括常规（c）蛋白激酶C亚型（α、βI、βII和γ）和新型（n）蛋白激酶C亚型（δ、ε、ζ、η、θ、λ/ι、µ和ν）。每种蛋白激酶C亚型在不同组织中的表达模式不同，蛋白激酶C家族成员在辅因子依赖性方面存在明显差异。例如，蛋白激酶C δ和ε的激酶活性不依赖于Ca2+。另一方面，大多数其他蛋白激酶C成员具有佛波酯结合活性和激酶活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC gamma 抗体 (C-4) 参考文献:

  1. 蛋白激酶 C 家族的新成员 nPKC theta 主要在骨骼肌中表达。  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  2. 信号转导蛋白激酶家族（蛋白激酶 C 家族）的结构和功能多样性；两类不同的 PKC，即传统的 cPKC 和新型的 nPKC。  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
  3. 蛋白激酶 C-delta 的表达和特征。  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
  4. 正常和阿尔茨海默氏症大脑中三种主要的人蛋白激酶C同工酶（PKC alpha、PKC beta和PKC gamma）的表征和差异分布。  |  Clark, EA., et al. 1991. Lab Invest. 64: 35-44. PMID: 1990207
  5. 钙信号、PKC Gamma、IP3R1 和 CAR8 将脊髓小脑共济失调与浦肯野细胞树突发育联系起来。  |  Shimobayashi, E. and Kapfhammer, JP. 2018. Curr Neuropharmacol. 16: 151-159. PMID: 28554312
  6. 分析PKC Gamma (+19,506 A/G)多态性，作为HCV诱导的肝细胞癌的潜在遗传标记。  |  Abid, F., et al. 2022. Biomark Res. 10: 87. PMID: 36451234
  7. AZU1（HBP/CAP37）和PRKCG（PKC-gamma）可能是影响急性高山病严重程度的候选基因。  |  Xu, Z., et al. 2023. BMC Med Genomics. 16: 28. PMID: 36803152
  8. 膜磷脂对多功能蛋白激酶的钙依赖性激活。  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  9. 肌醇磷脂的周转和信号转导  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  10. 蛋白激酶 C 在细胞表面信号转导和肿瘤促进中的作用。  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  11. 蛋白激酶 C 可能是促进肿瘤生长的植物醇酯的受体蛋白。  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  12. 促进肿瘤生长的植物醇酯直接激活钙激活的磷脂依赖性蛋白激酶。  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

订购信息

产品名称产品编号规格价格数量收藏夹

PKC gamma 抗体 (C-4)

sc-166385
200 µg/ml
RMB2377.00

PKC gamma (C-4): m-IgGκ BP-HRP 套装

sc-521584
200 µg Ab, 40 µg BP
RMB2662.00

PKC gamma (C-4): m-IgG2a BP-HRP 套装

sc-546100
200 µg Ab; 10 µg BP
RMB2662.00

PKC gamma 抗体 (C-4) AC

sc-166385 AC
500 µg/ml, 25% agarose
RMB3129.00

PKC gamma 抗体 (C-4) HRP

sc-166385 HRP
200 µg/ml
RMB2377.00

PKC gamma 抗体 (C-4) FITC

sc-166385 FITC
200 µg/ml
RMB2482.00

PKC gamma 抗体 (C-4) PE

sc-166385 PE
200 µg/ml
RMB2580.00

PKC gamma 抗体 (C-4) Alexa Fluor® 488

sc-166385 AF488
200 µg/ml
RMB2685.00

PKC gamma 抗体 (C-4) Alexa Fluor® 546

sc-166385 AF546
200 µg/ml
RMB2685.00

PKC gamma 抗体 (C-4) Alexa Fluor® 594

sc-166385 AF594
200 µg/ml
RMB2685.00

PKC gamma 抗体 (C-4) Alexa Fluor® 647

sc-166385 AF647
200 µg/ml
RMB2685.00

PKC gamma 抗体 (C-4) Alexa Fluor® 680

sc-166385 AF680
200 µg/ml
RMB2685.00

PKC gamma 抗体 (C-4) Alexa Fluor® 790

sc-166385 AF790
200 µg/ml
RMB2685.00

PKC gamma (C-4) 中和勝肽

sc-166385 P
100 µg/0.5 ml
RMB511.00

Will the phosphorylation at T674 site affect the binding of this antibody to PKCgamma?

Asked by: gogo
Thank you for your question. No, the phosphorylation site does not interfere with the detection of this antibody - it will detect both phosphorylated and unphosphorylated forms.
Answered by: Technical Service
Date published: 2017-04-20

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 7
Date published: 2017-02-07
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Rated 1 out of 5 by from Did not work for my IHCthere was no specific signals at all, disappointing.
Date published: 2021-06-16
Rated 5 out of 5 by from Great productWe used this antibody for our western blot. give us good results.
Date published: 2017-09-27
Rated 3 out of 5 by from Human PBMNI tested this antibody by western blot by using 0.2ug/ml in human peripheral blood mononuclear cell (PBMC). It gave 2 bands.
Date published: 2017-05-22
Rated 1 out of 5 by from Does not work in human or macaqueI tried a sample of the new monoclonal that has replaced the polyclonal used successfully by many research groups in my area. I was trying to visualise the corticospinal tract but this failed to work in paraffin sections from human post-mortem tissue or in fixed, frozen sections from macaque spinal cord.
Date published: 2017-03-22
Rated 5 out of 5 by from Great antibody for flow cytometryI used this antibody for flow cytometry using SupT1 cells
Date published: 2017-03-15
Rated 3 out of 5 by from works on IF of mice spinal dorsal hornI used 2 ug/ml to do immunofluorescence on mice spinal dorsal horn, the results seems comparable to the published data. The problem is the strong background at the ventral horn. I will try to modify the conditions and update the information later.
Date published: 2017-03-10
Rated 5 out of 5 by from The preferred choice for PKC gamma!This mouse monoclonal antibody, (C-4) clone, has been validated for detection of mouse, rat and human PKC gamma and is suitable for use in a broad range of research applications, most commonly Western blot and immunohistochemistry.
Date published: 2017-01-24
Rated 5 out of 5 by from Potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~80 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for a ~100 kDa protein.
Date published: 2016-11-10
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PKC gamma 抗体 (C-4) is rated 3.7 out of 5 by 9.
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