Date published: 2025-9-6
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PKC beta 2 Antibody (F-7) - Western Blotting - Image 2002
PKC βII (F-7): sc-13149. K-562 (A) 和 Jurkat (B) 全细胞裂解液中 PKC βII 表达的 Western 印迹分析.
PKC βII (F-7): sc-13149. K-562 (A) 和 Jurkat (B) 全细胞裂解液中 PKC βII 表达的 Western 印迹分析.

PKC beta 2 抗体 (F-7): sc-13149

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说明书
  • PKC beta 2抗体(F-7)是小鼠单克隆IgG1 κ, 在45篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PKC βII的C-terminus的氨基酸657-673之间
  • 抗-PKC beta 2 抗体 (F-7) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 cPKC βII; 也和以下物种反应，包括： 和 bovine and porcine
  • 抗PKC beta 2抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PKC beta 2 (F-7): sc-13149的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 PKC beta 2 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PKC beta 2 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PKC 抗体 包括 PKC α, PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ和PKC ν

PKC βII抗体（F-7）是一种小鼠单克隆IgG1 kappa轻链抗体，经过工程改造，能够以极高的精确度靶向PKC βII同种型蛋白激酶C。PKC βII 单克隆抗体（F-7）与位于人类 PKC βII C 末端 657-673 氨基酸之间的特定表位结合，确保精确检测和最小交叉反应。PKC βII 是蛋白激酶 C 家族的重要成员，在调节细胞生长、分化、凋亡、基因表达、激素分泌和膜功能等基本过程的多种细胞信号传导通路中发挥着关键作用。PKC βII (F-7) 抗体对多种物种均具有广泛的反应性，可有效识别小鼠、大鼠和人类样本中的 PKC βII，以及牛和猪等其他物种，适用于一系列应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHC/P）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。PKC βII (F-7) 单克隆抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，为研究人员提供了多种选择。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC beta 2 抗体 (F-7) 参考文献:

  1. 蛋白激酶 C 家族的新成员 nPKC theta 主要在骨骼肌中表达。  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  2. 信号转导蛋白激酶家族（蛋白激酶 C 家族）的结构和功能多样性；两类不同的 PKC，即传统的 cPKC 和新型的 nPKC。  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
  3. 蛋白激酶 C-delta 的表达和特征。  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
  4. 膜磷脂对多功能蛋白激酶的钙依赖性激活。  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  5. 肌醇磷脂的周转和信号转导  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  6. 蛋白激酶 C 在细胞表面信号转导和肿瘤促进中的作用。  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  7. 蛋白激酶 C 可能是促进肿瘤生长的植物醇酯的受体蛋白。  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  8. 促进肿瘤生长的植物醇酯直接激活钙激活的磷脂依赖性蛋白激酶。  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

订购信息

产品名称产品编号规格价格数量收藏夹

PKC beta 2 抗体 (F-7)

sc-13149
200 µg/ml
RMB2377.00

PKC beta 2 (F-7): m-IgG Fc BP-HRP 套装

sc-528271
200 µg Ab; 10 µg BP
RMB2662.00

PKC beta 2 (F-7): m-IgGκ BP-HRP 套装

sc-520610
200 µg Ab, 40 µg BP
RMB2662.00

PKC beta 2 抗体 (F-7) AC

sc-13149 AC
500 µg/ml, 25% agarose
RMB3129.00

PKC beta 2 抗体 (F-7) HRP

sc-13149 HRP
200 µg/ml
RMB2377.00

PKC beta 2 抗体 (F-7) FITC

sc-13149 FITC
200 µg/ml
RMB2482.00

PKC beta 2 抗体 (F-7) PE

sc-13149 PE
200 µg/ml
RMB2580.00

PKC beta 2 抗体 (F-7) Alexa Fluor® 488

sc-13149 AF488
200 µg/ml
RMB2685.00

PKC beta 2 抗体 (F-7) Alexa Fluor® 546

sc-13149 AF546
200 µg/ml
RMB2685.00

PKC beta 2 抗体 (F-7) Alexa Fluor® 594

sc-13149 AF594
200 µg/ml
RMB2685.00

PKC beta 2 抗体 (F-7) Alexa Fluor® 647

sc-13149 AF647
200 µg/ml
RMB2685.00

PKC beta 2 抗体 (F-7) Alexa Fluor® 680

sc-13149 AF680
200 µg/ml
RMB2685.00

PKC beta 2 抗体 (F-7) Alexa Fluor® 790

sc-13149 AF790
200 µg/ml
RMB2685.00

PKC beta 2 (F-7) 中和勝肽

sc-13149 P
100 µg/0.5 ml
RMB511.00

I have some monkey samples I would like to use for WB, do you know if this antibody will cross-react?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service 11
Date published: 2017-01-12

Do you have any tips for performing a double stain with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. This product, sc-13149, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Service 11
Date published: 2017-01-12
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Rated 5 out of 5 by from Worth investingHelped our Team's research in the experiments planned. Arrived on time and in a proper condition.
Date published: 2017-10-04
Rated 5 out of 5 by from good performanceThis PKC βII antibody very good performance. Without any need to optimiezed the protocol. It is specific and sensitive to the target protein at 80kda by 1:500.
Date published: 2017-09-02
Rated 5 out of 5 by from very useful antibodyThis anibody is cost effectiveness than other supplier’s antibody ,the result shows specific binding, high conceration at 1:1000.
Date published: 2017-07-08
Rated 5 out of 5 by from great antibody for western blottingWe used this antibody to examine its expression in postsynaptic density fractions purified from neonatal mouse brain by Western blotting. It worked very well!
Date published: 2017-05-24
Rated 5 out of 5 by from Good resultsGood results by WB for human PBMC, showed single band with exact molecular weight.
Date published: 2017-05-20
Rated 5 out of 5 by from High effencySingle strong band at correct mw in human liver tissue, very good antibody.
Date published: 2017-04-29
Rated 5 out of 5 by from Very efficientPKC βII antibody works well. We use dilution 1:500 got specific band,strong wb with low background, also good for staining.
Date published: 2017-04-16
Rated 5 out of 5 by from Antibody is really greatAntibody provides clear and specific results,antibody is really great.
Date published: 2017-02-06
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PKC beta 2 抗体 (F-7) is rated 4.9 out of 5 by 18.
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