Date published: 2025-9-6
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PKC beta 1 Antibody (E-3) - Western Blotting - Image 370728
PKC βI 抗体 (E-3) HRP: sc-8049 HRP. 在 MOLT-4 (A), A-431 (B), Jurkat (C) 和 NIH/3T3 (D) 全细胞裂解液以及小鼠大脑 (E) 和小鼠小脑 (F) 组织提取物中 PKC βI 表达的直接 Western 印迹分析.
PKC βI 抗体 (E-3) HRP: sc-8049 HRP. 在 MOLT-4 (A), A-431 (B), Jurkat (C) 和 NIH/3T3 (D) 全细胞裂解液以及小鼠大脑 (E) 和小鼠小脑 (F) 组织提取物中 PKC βI 表达的直接 Western 印迹分析.

PKC beta 1 抗体 (E-3): sc-8049

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说明书
  • PKC beta 1 抗体 E-3 是小鼠单克隆 IgG1 κ，PKC beta 1抗体, 在51篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PKC βI的C-terminus的氨基酸656-671之间
  • PKC beta 1 抗体 (E-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的PKC βI; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian
  • 抗PKC beta 1抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PKC beta 1 (E-3): sc-8049的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 PKC beta 1 抗体 (E-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PKC beta 1 抗体 (E-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PKC 抗体 包括 PKC α, PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ和PKC ν

PKC βI 抗体（E-3）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源的 PKC βI 蛋白。PKC βI (E-3) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。PKC beta I蛋白（也称为PRKCB）是蛋白激酶C（PKC）家族的重要成员，在调节细胞生长、分化、基因表达和激素分泌等多种细胞功能方面发挥着重要作用。PKC beta I 主要位于细胞质中，激活后转移到膜上，这对于其在信号转导通路中的作用至关重要。这种转移使PKC beta I能够与各种底物相互作用，并参与关键的细胞过程，包括调节细胞存活和凋亡。PKC beta I的激活受二酰基甘油和苯甲酸酯的影响，它们与PKC beta I结合并促进激酶活性。了解PKC βI的定位和功能对于阐明其在包括癌症和心血管疾病在内的各种疾病中的参与情况非常重要，这使得PKC βI (E-3)单克隆抗体成为研究人员研究这些途径的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC beta 1 抗体 (E-3) 参考文献:

  1. 蛋白激酶 C 家族的新成员 nPKC theta 主要在骨骼肌中表达。  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  2. 信号转导蛋白激酶家族（蛋白激酶 C 家族）的结构和功能多样性；两类不同的 PKC，即传统的 cPKC 和新型的 nPKC。  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
  3. 蛋白激酶 C-delta 的表达和特征。  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
  4. 膜磷脂对多功能蛋白激酶的钙依赖性激活。  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  5. 肌醇磷脂的周转和信号转导  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  6. 蛋白激酶 C 在细胞表面信号转导和肿瘤促进中的作用。  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  7. 蛋白激酶 C 可能是促进肿瘤生长的植物醇酯的受体蛋白。  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  8. 促进肿瘤生长的植物醇酯直接激活钙激活的磷脂依赖性蛋白激酶。  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

订购信息

产品名称产品编号规格价格数量收藏夹

PKC beta 1 抗体 (E-3)

sc-8049
200 µg/ml
RMB2377.00

PKC beta 1 (E-3): m-IgG Fc BP-HRP 套装

sc-528214
200 µg Ab; 10 µg BP
RMB2662.00

PKC beta 1 (E-3): m-IgGκ BP-HRP 套装

sc-520537
200 µg Ab, 40 µg BP
RMB2662.00

PKC beta 1 抗体 (E-3) AC

sc-8049 AC
500 µg/ml, 25% agarose
RMB3129.00

PKC beta 1 抗体 (E-3) HRP

sc-8049 HRP
200 µg/ml
RMB2377.00

PKC beta 1 抗体 (E-3) FITC

sc-8049 FITC
200 µg/ml
RMB2482.00

PKC beta 1 抗体 (E-3) PE

sc-8049 PE
200 µg/ml
RMB2580.00

PKC beta 1 抗体 (E-3) Alexa Fluor® 488

sc-8049 AF488
200 µg/ml
RMB2685.00

PKC beta 1 抗体 (E-3) Alexa Fluor® 546

sc-8049 AF546
200 µg/ml
RMB2685.00

PKC beta 1 抗体 (E-3) Alexa Fluor® 594

sc-8049 AF594
200 µg/ml
RMB2685.00

PKC beta 1 抗体 (E-3) Alexa Fluor® 647

sc-8049 AF647
200 µg/ml
RMB2685.00

PKC beta 1 抗体 (E-3) Alexa Fluor® 680

sc-8049 AF680
200 µg/ml
RMB2685.00

PKC beta 1 抗体 (E-3) Alexa Fluor® 790

sc-8049 AF790
200 µg/ml
RMB2685.00

PKC beta 1 (E-3) 中和勝肽

sc-8049 P
100 µg/0.5 ml
RMB511.00

실험중에 의문이 생겨 문의드립니다. pkc beta 1 사이즈가 79 라고 하는데 사이트 사진처럼 75, 90 이렇게 두개에 밴드가 생겨서 두 밴드의 차이를 여쭙고 싶어 문의드립니다.

Asked by: juhyeon
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2024-08-23

Can the blocking peptide be used as a positive control in Western Blot?

Asked by: AbPolly
Thank you for your question. The blocking peptide, sc-8049 P, is a short synthetic peptide and is intended for use as a negative control. It is not appropriate for use as a positive control in Western Blot.
Answered by: Technical Support 9
Date published: 2017-01-24
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Rated 5 out of 5 by from GoodI tested this antibody in Jan 2018. The bands are clear and the pattern detected matches the data sheet.
Date published: 2018-02-13
Rated 5 out of 5 by from It's perfectWe get specific signal for the protein at the right molecular weight when we use PKC βI Antibody of the cell. It's the very efficient antibody indeed.
Date published: 2017-10-21
Rated 5 out of 5 by from The antibody is really greatPKC βI Antibody is highly suitable to show cellular staining. Show us the strong signal with low background and the baseband is perfect.
Date published: 2017-10-18
Rated 4 out of 5 by from good antibodyUsed the antibody at the WB,it is very useful for my experiment! And we will buy some.
Date published: 2017-10-11
Rated 3 out of 5 by from slightly workedOverexpressed HA-PKCbI in 293 cells was weakly detected. HA-PKCa was cross reacted. endogenous PKCbI was weakly detected in 293 cell lysates. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-07-05
Rated 5 out of 5 by from helpful for my experimentUsed the antibody at the WB,it is very useful for my experiment!
Date published: 2017-06-21
Rated 3 out of 5 by from Good resultsI tested this antibody by western blot by using 0.2ug/ml in human peripheral blood mononuclear cell (PBMC). good for WB
Date published: 2017-05-22
Rated 5 out of 5 by from great antibody for flow cytometryThis antibody worked very well for flow cytometry using SupT1 cells
Date published: 2017-03-07
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PKC beta 1 抗体 (E-3) is rated 4.6 out of 5 by 14.
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