Date published: 2025-9-6
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PKC alpha Antibody (H-7) - Western Blotting - Image 387579
同时对 MOLT-4 (A), Jurkat (B), HeLa (C) 和 NIH/3T3 (D) 全细胞裂解液中 PKC α 表达 (用 PKC α 抗体 (H-7) Alexa Fluor® 790: sc-8393 AF790 检测) 和 GAPDH 表达 (用 GAPDH (G-9) Alexa Fluor® 680: sc-365062 AF680 检测) 进行直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
同时对 MOLT-4 (A), Jurkat (B), HeLa (C) 和 NIH/3T3 (D) 全细胞裂解液中 PKC α 表达 (用 PKC α 抗体 (H-7) Alexa Fluor® 790: sc-8393 AF790 检测) 和 GAPDH 表达 (用 GAPDH (G-9) Alexa Fluor® 680: sc-365062 AF680 检测) 进行直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

PKC alpha 抗体 (H-7): sc-8393

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说明书
  • PKC alpha 抗体 H-7 是小鼠单克隆 IgG1 κ，PKC alpha抗体, 在324篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PKC α的C-terminus的氨基酸651-672之间
  • PKC alpha 抗体 (H-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PKC α
  • 抗PKC alpha抗体（H-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PKC alpha (H-7): sc-8393的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 PKC alpha 抗体 (H-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PKC alpha 抗体 (H-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PKC 抗体 包括 PKC α, PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ和PKC ν

PKC α 抗体（H-7）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源的 PKC α 蛋白。PKC α 抗体（H-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物。蛋白激酶C（PKC）家族成员之一PKCα蛋白在调节细胞生长、分化、基因表达和激素分泌等许多细胞功能方面发挥着至关重要的作用，因此PKCα对于维持细胞稳态至关重要。PKC alpha 主要位于细胞质中，激活后转移到细胞膜，这是 PKC alpha 在信号传导通路中发挥作用的关键。这种转移是由二酰基甘油（DAG）和肿瘤促进剂福尔博酯引发的，它们与 PKC alpha 结合并激活它，使 PKC alpha 能够磷酸化目标蛋白并调节各种细胞反应。PKC同工型在不同组织中的不同表达模式凸显了PKCα在特定信号通路中的重要性，而PKCα独特的辅助因子依赖性进一步强调了PKCα在PKC家族中独特的调节机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC alpha 抗体 (H-7) 参考文献:

  1. 蛋白激酶C-α（PKC-α）在黑色素瘤中的作用。  |  Lahn, MM. and Sundell, KL. 2004. Melanoma Res. 14: 85-9. PMID: 15057036
  2. 蛋白激酶 C 家族的新成员 nPKC theta 主要在骨骼肌中表达。  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  3. 白藜芦醇调节胃癌细胞中的细胞PKCα和δ，抑制其生长并诱导其凋亡。  |  Atten, MJ., et al. 2005. Invest New Drugs. 23: 111-9. PMID: 15744586
  4. 信号转导蛋白激酶家族（蛋白激酶 C 家族）的结构和功能多样性；两类不同的 PKC，即传统的 cPKC 和新型的 nPKC。  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
  5. 蛋白激酶 C-delta 的表达和特征。  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
  6. PKC-α调节TGF-β信号并影响足细胞存活。  |  Tossidou, I., et al. 2009. Cell Physiol Biochem. 24: 627-34. PMID: 19910703
  7. 2019年冠状病毒病（COVID-19）, 人类红细胞和PKC-α/β抑制剂白屈菜红碱（chelerythrine）--可能的治疗意义。  |  Ghashghaeinia, M., et al. 2020. Cell Cycle. 19: 3399-3405. PMID: 33305655
  8. 膜磷脂对多功能蛋白激酶的钙依赖性激活。  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  9. 肌醇磷脂的周转和信号转导  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  10. 蛋白激酶 C 在细胞表面信号转导和肿瘤促进中的作用。  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  11. 蛋白激酶 C 可能是促进肿瘤生长的植物醇酯的受体蛋白。  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  12. 促进肿瘤生长的植物醇酯直接激活钙激活的磷脂依赖性蛋白激酶。  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

订购信息

产品名称产品编号规格价格数量收藏夹

PKC alpha 抗体 (H-7)

sc-8393
200 µg/ml
RMB2377.00

PKC alpha (H-7): m-IgG Fc BP-HRP 套装

sc-528219
200 µg Ab; 10 µg BP
RMB2662.00

PKC alpha (H-7): m-IgGκ BP-HRP 套装

sc-520544
200 µg Ab, 40 µg BP
RMB2662.00

PKC alpha (H-7): m-IgG1 BP-HRP 套装

sc-542822
200 µg Ab; 20 µg BP
RMB2662.00

PKC alpha 抗体 (H-7) AC

sc-8393 AC
500 µg/ml, 25% agarose
RMB3129.00

PKC alpha 抗体 (H-7) HRP

sc-8393 HRP
200 µg/ml
RMB2377.00

PKC alpha 抗体 (H-7) FITC

sc-8393 FITC
200 µg/ml
RMB2482.00

PKC alpha 抗体 (H-7) PE

sc-8393 PE
200 µg/ml
RMB2580.00

PKC alpha 抗体 (H-7) Alexa Fluor® 488

sc-8393 AF488
200 µg/ml
RMB2685.00

PKC alpha 抗体 (H-7) Alexa Fluor® 546

sc-8393 AF546
200 µg/ml
RMB2685.00

PKC alpha 抗体 (H-7) Alexa Fluor® 594

sc-8393 AF594
200 µg/ml
RMB2685.00

PKC alpha 抗体 (H-7) Alexa Fluor® 647

sc-8393 AF647
200 µg/ml
RMB2685.00

PKC alpha 抗体 (H-7) Alexa Fluor® 680

sc-8393 AF680
200 µg/ml
RMB2685.00

PKC alpha 抗体 (H-7) Alexa Fluor® 790

sc-8393 AF790
200 µg/ml
RMB2685.00

PKC alpha (H-7) 中和勝肽

sc-8393 P
100 µg/0.5 ml
RMB511.00

does this product work on zebrafish embryo?any suggestion for zebrafish use,the product SC-216 is not available now!

Asked by: liyana
Thank you for your question. We are not aware of any publications citing use of this mouse monoclonal antibody, PKC α (H-7): sc-8393, in zebrafish samples. The antibody is raised against a peptide that does share significant (76%) sequence identity with PKC α in zebrafish, so there is a good chance that it would work in this species. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a sample to test in zebrafish.
Answered by: Technical Support
Date published: 2017-07-18

What secondary detection reagent should be used with PKC α Antibody (H-7) for Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2021-10-29
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Rated 5 out of 5 by from well function in WB and IFGood results when tested PKC alpha in western blot of whole cell extracts or IFfrom rat articular chondrocytes.
Date published: 2023-12-22
Rated 5 out of 5 by from Dendritic morphology of bipolar cells in wild type10 to 15 weeks old mice were anesthetized with isoflurane (Vetflurane®k, Virbac) and killed by cervical dislocation. Vertical sections: The following steps were conducted at room temperature if not stated otherwise. Eyes were quickly removed from the eye socket, opened at the scleral-corneal rim and immersed for 10 min with 4% paraformaldehyde (PFA) in 1X phosphate-buffered saline (1X PBS, pH 7.4). Cornea, lens and vitreous were removed. Eye cups were then fixed with 4%PFA/1X PBS for 20 min, washed four times with 1X PBS and cryoprotected by a graded sucrose series: 10% sucrose in 1X PBS for 1 h, 20% sucrose in 1X PBS for 1 h and 30% sucrose in 1X PBS overnight at 4 °C. Eyecups were orientated along the dorsoven-tral axis, embedded in OCT Medium (Tissue-Tek O.C.T Compound; Sakura Finetek, Tokyo, Japan) and frozen in liquid nitrogen. Vertical sections (16 μm) were cut on a cryostat (Leica Microsystems, Wetzlar, Germany), mounted on gelatine coated slides and stored at − 20 °C. For immunofluorescence experiments, sections were washed three times in 1X PBS-T (1X PBS + 0.1% Triton X-100, Sigma-Aldrich, St. Louis, MO, USA), blocked for 1 h in 1X PBS-T containing 1% bovine serum albumin (BSA, Sigma-Aldrich, A7030) and incubated overnight at 4 °C with primary antibodies diluted in 1X PBS-T at concentrations listed in Supplementary table 1. After wash-ing three times with 1X PBS-T, sections were incubated with the secondary antibodies (Supplementary table 2) for one. Additional washes preceded the counterstaining with DAPI (1:10,000; Sigma, D-9542) and eventually the sections were mounted using Poly/Mount (Polysciences, Inc., Warrington, PA, USA)
Date published: 2022-01-17
Rated 5 out of 5 by from Works great for IHC in mouse retinaWe use this antibody as standard marker in our lab. Very intense and nice staining of bipolar cells in the retina
Date published: 2022-01-05
Rated 5 out of 5 by from Works very good!Used this antibody for IF (1:50/1:100/1:200) on cryosections (10µm) from human retinal organoids and obtained nice images (for all dilutions)!
Date published: 2019-07-23
Rated 5 out of 5 by from Works great on pig retinaPKCa staining (IHC-Fr) on 8um thick pig retina for bipolar cells Blocking: 5% donkey serum P Ab: 1:50 (overnight) S Ab: anti-mouse 1:200 (1h)
Date published: 2017-10-23
Rated 5 out of 5 by from Nice AntibodyPKC α Antibody gives very good signal and manipulating conveniently with good control effect.The clarity of result can be  too good for us.
Date published: 2017-10-18
Rated 5 out of 5 by from Great productWe used this antibody for our western blot. give us good results.
Date published: 2017-09-27
Rated 5 out of 5 by from IHCThe relationship between the expression of PKC- alpha protein and the clinicopathological features of lung cancer was detected by IHC with this antibody.It is found that PKC-A is closely related to the development of lung cancer. We found PKA-A canbe used assit for lung cancer diagnosis.We recommend the use of 1:300 for immunohistochemistry.
Date published: 2017-09-16
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PKC alpha 抗体 (H-7) is rated 4.8 out of 5 by 34.
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