Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
phospholamban Antibody (F-7) - Western Blotting - Image 300119
phospholamban 抗体 (F-7): sc-393990. 小鼠心脏 (A) 和人类心脏 (B) 组织提取物中磷脂兰班表达的 Western 印迹分析.
phospholamban 抗体 (F-7): sc-393990. 小鼠心脏 (A) 和人类心脏 (B) 组织提取物中磷脂兰班表达的 Western 印迹分析.

phospholamban 抗体 (F-7): sc-393990

5.0(4)
寫評論提問

说明书
  • phospholamban 抗体 F-7 是小鼠单克隆 IgG1 κ，phospholamban抗体, 在13篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的phospholamban的N-terminus的氨基酸7-32之间
  • phospholamban 抗体 (F-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 phospholamban
  • 抗phospholamban抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取phospholamban (F-7): sc-393990的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 phospholamban 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与phospholamban 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

磷蛋白抗体（F-7）是一种IgG1 κ小鼠单克隆磷蛋白抗体（也称为PLN抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人类来源的磷蛋白。磷蛋白抗体（F-7）既有非偶联形式的抗磷蛋白抗体，也有多种偶联形式的抗磷蛋白抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肌（内）质网（SER）调节蛋白磷蛋白（PLB）是一种小的、与质膜相关的磷酸蛋白，存在于心脏、平滑肌和慢收缩肌的SER中。磷蛋白被认为可以组装成五聚体，调节心肌收缩力和心脏SER Ca2+ ATPase（SERCA2a）对Ca2+的亲和力。非磷酸化的磷蛋白与SERCA2a结合，抑制Ca2+重新摄入SER。当磷蛋白中的关键丝氨酸/苏氨酸残基（Ser 10、Ser 16、Thr 17）被多种效应物（包括PKC、PKA、PKG和CaM激酶）磷酸化时，磷蛋白被激活。磷蛋白的磷酸化导致其从SERCA2a解离，随后增加SER中Ca2+重新摄入的速率，从而加速心室舒张。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

phospholamban 抗体 (F-7) 参考文献:

  1. 慢性磷脂酰胆碱-肌浆网钙ATP酶相互作用是扩张型心肌病的关键钙循环缺陷。  |  Minamisawa, S., et al. 1999. Cell. 99: 313-22. PMID: 10555147
  2. 磷脂酰班的磷酸化状态。  |  Colyer, J. 1998. Ann N Y Acad Sci. 853: 79-91. PMID: 10603938
  3. 磷脂酰班五聚体跨膜区的模型：哪个正确？  |  Adams, PD., et al. 1998. Ann N Y Acad Sci. 853: 178-85. PMID: 10603945
  4. 心脏特异性过表达超抑制性五聚磷脂酰班突变体可增强对体内心脏功能的抑制。  |  Zhai, J., et al. 2000. J Biol Chem. 275: 10538-44. PMID: 10744747
  5. 磷酸肌球蛋白和心脏收缩力。  |  Frank, K. and Kranias, EG. 2000. Ann Med. 32: 572-8. PMID: 11127935
  6. 磷酸肌钙蛋白反义寡核苷酸可改善小鼠心肌病的心脏功能。  |  Grote Beverborg, N., et al. 2021. Nat Commun. 12: 5180. PMID: 34462437
  7. 磷酸肌球蛋白五聚体化可提高心脏舒张的敏感性和动态范围。  |  Funk, F., et al. 2023. Cardiovasc Res. 119: 1568-1582. PMID: 36869774
  8. ZBTB20通过磷酸肌球蛋白调节SERCA2a活性和心肌收缩性。  |  Ren, AJ., et al. 2024. Circ Res. 134: 252-265. PMID: 38166470
  9. 磷酸肌钙蛋白突变PLN-R14del+/-在转基因小鼠模型中的早期后果。  |  Maniezzi, C., et al. 2024. Acta Physiol (Oxf). 240: e14082. PMID: 38214033
  10. 寻找磷酸肌球蛋白表达的调节机制。  |  Mundiña-Weilenmann, C. 2024. Circ Res. 134: 266-268. PMID: 38300986
  11. 磷脂酰班：心肌收缩力的主要调节器。  |  Koss, KL. and Kranias, EG. 1996. Circ Res. 79: 1059-63. PMID: 8943944
  12. 螺旋跨膜五聚体 phospholamban 的结构展望。  |  Arkin, IT., et al. 1997. Annu Rev Biophys Biomol Struct. 26: 157-79. PMID: 9241417

订购信息

产品名称产品编号规格价格数量收藏夹

phospholamban 抗体 (F-7)

sc-393990
200 µg/ml
RMB2377.00

phospholamban (F-7): m-IgG Fc BP-HRP 套装

sc-530650
200 µg Ab; 10 µg BP
RMB2662.00

phospholamban (F-7): m-IgGκ BP-HRP 套装

sc-524169
200 µg Ab, 40 µg BP
RMB2662.00

phospholamban (F-7): m-IgG1 BP-HRP 套装

sc-544179
200 µg Ab; 20 µg BP
RMB2662.00

phospholamban 抗体 (F-7) AC

sc-393990 AC
500 µg/ml, 25% agarose
RMB3129.00

phospholamban 抗体 (F-7) HRP

sc-393990 HRP
200 µg/ml
RMB2377.00

phospholamban 抗体 (F-7) FITC

sc-393990 FITC
200 µg/ml
RMB2482.00

phospholamban 抗体 (F-7) PE

sc-393990 PE
200 µg/ml
RMB2580.00

phospholamban 抗体 (F-7) Alexa Fluor® 488

sc-393990 AF488
200 µg/ml
RMB2685.00

phospholamban 抗体 (F-7) Alexa Fluor® 546

sc-393990 AF546
200 µg/ml
RMB2685.00

phospholamban 抗体 (F-7) Alexa Fluor® 594

sc-393990 AF594
200 µg/ml
RMB2685.00

phospholamban 抗体 (F-7) Alexa Fluor® 647

sc-393990 AF647
200 µg/ml
RMB2685.00

phospholamban 抗体 (F-7) Alexa Fluor® 680

sc-393990 AF680
200 µg/ml
RMB2685.00

phospholamban 抗体 (F-7) Alexa Fluor® 790

sc-393990 AF790
200 µg/ml
RMB2685.00

phospholamban (F-7) 中和勝肽

sc-393990 P
100 µg/0.5 ml
RMB511.00

phospholamban 抗体 (F-7): sc-393990，免疫荧光染色细胞定位在哪里？内质网膜吗

Asked by: jiajia12
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2020-01-07

Will the phospholamban antibody (F-7): sc-393990 work to detect this protein in Avian samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 10
Date published: 2017-01-11

Why is phospholamban antibody (F-7): sc-393990 the recommended alternative to the now discontinued phospholamban (L-15): sc-21923?

Asked by: Trav11
Thank you for your question. The mouse monoclonal phospholamban antibody (F-7): sc-393990 is raised against a similar immunogen sequence as the goat polyclonal phospholamban (L-15): sc-21923. The monoclonal primary antibody also offers a higher degree of specificity and eliminates lot-to-lot variation. It is warrantied for all of the same applications as the discontinued polyclonal antibody: WB, IP, IF, IHC(P) and ELISA. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 10
Date published: 2017-01-11
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_393990, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 105ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from IF stainingThis antibody was used to IF staining HeLa cells. The antibody was specific and showed clear immunoreactive staining that is more better result thant the expensive anti phospholamban antibody.I only use the dilution 1:300 ,still specific staining.
Date published: 2017-07-29
Rated 5 out of 5 by from Outstanding !!Superb western data; strong signal with no background; also great IHC; far better than polyclonal alternative
Date published: 2017-01-15
Rated 5 out of 5 by from Good staining strengthStrong staining seen in human heart tissue in IHC(P)
Date published: 2016-12-23
Rated 5 out of 5 by from Great Western blot data of phospholambanGreat Western blot data of phospholamban expression in mouse heart and human heart tissue extracts. -SCBT QC
Date published: 2013-03-26
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_393990, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
phospholamban 抗体 (F-7) is rated 5.0 out of 5 by 4.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_393990, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。