Date published: 2025-9-8

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Perlecan 抗体 (E-6): sc-377219

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说明书
  • Perlecan 抗体 E-6 是小鼠单克隆 IgG1 κ,Perlecan抗体, 在12篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列4092-4391位于human物种的Perlecan的C-terminus附近
  • Perlecan 抗体 (E-6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Perlecan
  • 抗Perlecan抗体(E-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Perlecan (E-6): sc-377219免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Perlecan 抗体 (E-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Perlecan 抗体 (E-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Perlecan 抗体 (E-6) 是一种 IgG1 κ 小鼠单克隆 Perlecan 抗体(也称为 HSPG2 抗体、Schwartz-Jampel 综合征 1(软骨营养不良性肌强直)抗体、SJS 抗体、SJS1 抗体、HSPG 抗体、PLC 抗体或 PRCAN 抗体),可通过 WB、IP、IF、IHC(P) 和 ELISA 检测人源 Perlecan 蛋白。Perlecan 抗体 (E-6) 可作为非偶联的抗 Perlecan 抗体形式,以及多种偶联形式的抗 Perlecan 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。Perlecan是硫酸乙酰肝素蛋白多糖(HSPG)大家族的一部分。作为细胞表面和细胞外基质的关键成分,HSPG调节生长因子活性,从而影响细胞生长和分化。此外,HSPG通过介导细胞粘附以及生长和血管生成因子的活性,在调节肿瘤细胞转移中发挥着关键作用。Perlecan由五个不同的结构域组成,这些结构域与许多基质分子、细胞因子和生长因子相互作用,影响软骨发育和神经肌肉接头活性。抗凝血酶是凝血蛋白酶的关键调节因子,TGFβ1分别作为Perlecan表达的抑制剂和刺激因子,相互作用可能为某些类型的癌症的治疗干预提供途径。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Perlecan 抗体 (E-6) 参考文献:

  1. Perlecan在软骨形成过程中的功能:体外和体内模型的启示。  |  Gomes, RR., et al. 2004. Cells Tissues Organs. 176: 79-86. PMID: 14745237
  2. 子痫前期妇女的母体血清 'perlecan '水平。  |  Akbas, M., et al. 2020. Hypertens Pregnancy. 39: 70-76. PMID: 31899995
  3. Perlecan Domain-V 可增强小鼠中风后的神经源性脑修复。  |  Trout, AL., et al. 2021. Transl Stroke Res. 12: 72-86. PMID: 32253702
  4. Perlecan促进神经元一氧化氮合酶在去神经支配诱导的肌肉萎缩中的异地化  |  Nakada, S., et al. 2020. Cells. 9: PMID: 33238404
  5. 核Perlecan和其他硫酸肝素蛋白聚糖在正常和恶性表型中的潜在作用是什么?  |  Hayes, AJ. and Melrose, J. 2021. Int J Mol Sci. 22: PMID: 33922532
  6. 缺乏 Perlecan 会增加主动脉夹层的风险。  |  Nonaka, R., et al. 2021. Int J Mol Sci. 23: PMID: 35008739
  7. 角膜纤维化中与perlecan相关的基底膜再生缺陷和转化生长因子β的调节改变。  |  Wilson, SE. 2022. Cell Mol Life Sci. 79: 144. PMID: 35188596
  8. 重组人 Perlecan DV 及其 LG3 亚域对严重实验性缺血性中风具有神经保护和急性功能恢复作用。  |  Biose, IJ., et al. 2023. Transl Stroke Res. 14: 941-954. PMID: 36508132
  9. 经改造的短型perlecan可通过增强生长因子信号来促进血管生成。  |  Kim, HN., et al. 2023. J Control Release. 362: 184-196. PMID: 37648081
  10. 硫酸肝素蛋白多糖 Perlecan 对糖蛋白 VI 依赖性血小板活化和血栓形成的调节作用  |  Provenzale, I., et al. 2023. Int J Mol Sci. 24: PMID: 37686158

订购信息

产品名称产品编号规格价格数量收藏夹

Perlecan 抗体 (E-6)

sc-377219
200 µg/ml
RMB2377.00

Perlecan (E-6): m-IgGκ BP-HRP 套装

sc-523237
200 µg Ab, 40 µg BP
RMB2662.00

Perlecan (E-6): m-IgG1 BP-HRP 套装

sc-533565
200 µg Ab; 20 µg BP
RMB2662.00

Perlecan 抗体 (E-6) AC

sc-377219 AC
500 µg/ml, 25% agarose
RMB3129.00

Perlecan 抗体 (E-6) HRP

sc-377219 HRP
200 µg/ml
RMB2377.00

Perlecan 抗体 (E-6) FITC

sc-377219 FITC
200 µg/ml
RMB2482.00

Perlecan 抗体 (E-6) PE

sc-377219 PE
200 µg/ml
RMB2580.00

Perlecan 抗体 (E-6) Alexa Fluor® 488

sc-377219 AF488
200 µg/ml
RMB2685.00

Perlecan 抗体 (E-6) Alexa Fluor® 546

sc-377219 AF546
200 µg/ml
RMB2685.00

Perlecan 抗体 (E-6) Alexa Fluor® 594

sc-377219 AF594
200 µg/ml
RMB2685.00

Perlecan 抗体 (E-6) Alexa Fluor® 647

sc-377219 AF647
200 µg/ml
RMB2685.00

Perlecan 抗体 (E-6) Alexa Fluor® 680

sc-377219 AF680
200 µg/ml
RMB2685.00

Perlecan 抗体 (E-6) Alexa Fluor® 790

sc-377219 AF790
200 µg/ml
RMB2685.00

您好,我能先申请一个试用装试用一下吗,因为之前有买其他家的这个抗体一直不太好跑,这次我可以先试试能不能跑出来再买产品吗,希望您能让我申请一个试用装。

Asked by: 张 世 莹
感谢您的提问。您需要购买其他一抗,随单申请这个抗体的试用装。如果有其他问题,请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. Please order the other primary antibody and apply for sc-377219 sample. If you have any other question, please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Sen Li
Date published: 2024-10-17

IHC(P) : what method do you recommend for antigen retrieval on a human skin explant ?

Asked by: Ellie
Thank you for your question. Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0.
Answered by: BlakeJ
Date published: 2024-05-24

Can Perlecan (E-6): sc-377219 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Perlecan (E-6): sc-377219 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from good antibodyantibody clean and specific - tested on human primary muscle cells - IF
Date published: 2018-04-04
Rated 5 out of 5 by from Works perfectly for IHC-PStained at 1:50 (IHC-P) for cells embedded in a gel and it worked perfectly fine.
Date published: 2018-02-21
Rated 4 out of 5 by from works great on western blot and IHCWe have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 5 out of 5 by from Nice Western blot data of Perlecan expressionNice Western blot data of Perlecan expression in HeLa nuclear extract. -SCBT QC
Date published: 2014-02-13
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Perlecan 抗体 (E-6) is rated 4.5 out of 5 by 4.
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