Date published: 2025-9-8
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Perlecan Antibody (A7L6) - Immunofluorescence - Image 12155
Perlecan 抗体 (A7L6): sc-33707. 正常小鼠眼球冰冻切片的免疫荧光染色, 显示基底膜, 基质和德氏膜染色.
Perlecan Antibody (A7L6) - Immunofluorescence - Image 12155
Perlecan 抗体 (A7L6): sc-33707. 正常小鼠眼球冰冻切片的免疫荧光染色, 显示基底膜, 基质和德氏膜染色.
Perlecan 抗体 (A7L6): sc-33707. 正常小鼠眼球冰冻切片的免疫荧光染色, 显示基底膜, 基质和德氏膜染色.

Perlecan 抗体 (A7L6): sc-33707

4.1(7)
寫評論提問

说明书
  • Perlecan 抗体 A7L6 是大鼠单克隆 IgG2a, 在27篇文献中引用，提供 200 µg/ml
  • 针对部分纯化的EHS小鼠肿瘤层粘连蛋白制剂
  • 抗-Perlecan 抗体 (A7L6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human, porcine, bovine, monkey 和fish 来源的 perlecan
  • 抗Perlecan抗体（A7L6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Perlecan (A7L6): sc-33707的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Perlecan 抗体 (A7L6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Perlecan抗体（A7L6）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠、人、猪、牛、猴和鱼样本中的perlecan。抗perlecan抗体（A7L6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Perlecan是硫酸肝素蛋白多糖家族的一员，在细胞外基质中起着至关重要的作用，它参与调节生长因子的活性，从而影响细胞的生长和分化。Perlecan的独特结构由五个不同的结构域组成，能够与各种基质分子、细胞因子和生长因子相互作用，这对于软骨发育和神经肌肉连接的正常运作至关重要。Perlecan与抗凝血酶和TGFβ1的相互作用分别强调了其在血液凝固和细胞信号传导中的调节作用，这表明了解这些相互作用可能会为癌症和其他疾病带来新的治疗策略。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Perlecan 抗体 (A7L6) 参考文献:

  1. perlecan在骨骼发育和疾病中的作用。  |  Hassell, J., et al. 2002. Glycoconj J. 19: 263-7. PMID: 12975604
  2. Perlecan 和肿瘤血管生成  |  Jiang, X. and Couchman, JR. 2003. J Histochem Cytochem. 51: 1393-410. PMID: 14566013
  3. 骨骼肌再生过程中硫酸肝素蛋白多糖增加：成功形成纤维需要辛迪加-3。  |  Casar, JC., et al. 2004. J Cell Sci. 117: 73-84. PMID: 14627628
  4. 肝素酶降解辛迪加-1 和perlecan肝素硫酸酯：对肿瘤细胞侵袭的功能性影响。  |  Reiland, J., et al. 2004. J Biol Chem. 279: 8047-55. PMID: 14630925
  5. perlecan在纤维化中的多方面作用。  |  Lord, MS., et al. 2018. Matrix Biol. 68-69: 150-166. PMID: 29475023
  6. 模块蛋白多糖 Perlecan/HSPG2：突变、表型和功能  |  Martinez, JR., et al. 2018. Genes (Basel). 9: PMID: 30453502
  7. Perlecan，一种具有多种功能特性的模块化指令蛋白多糖。  |  Melrose, J. 2020. Int J Biochem Cell Biol. 128: 105849. PMID: 32947020
  8. 硫酸肝素蛋白多糖 'perlecan '对人类疾病和健康的影响。  |  Arikawa-Hirasawa, E. 2022. Am J Physiol Cell Physiol. 322: C1117-C1122. PMID: 35417267
  9. Perlecan是一种多功能的、对细胞有指导作用的基质稳定蛋白多糖，在组织发育过程中发挥作用，与结缔组织修复和再生有关。  |  Hayes, AJ., et al. 2022. Front Cell Dev Biol. 10: 856261. PMID: 35433700
  10. Perlecan：综述其在神经和肌肉骨骼疾病中的作用。  |  Lavorgna, TR., et al. 2023. Front Physiol. 14: 1189731. PMID: 37324385
  11. Perlecan（HSPG2）可促进人类心肌细胞的结构、收缩和代谢发育。  |  Johnson, BB., et al. 2024. Cell Rep. 43: 113668. PMID: 38198277
  12. 抗凝血酶能下调血管内皮细胞中促血管生成的硫酸肝素蛋白多糖 'perlecan '的表达。  |  Weiqing Zhang,, et al. 2004. Blood. 103: 1185–1191.

订购信息

产品名称产品编号规格价格数量收藏夹

Perlecan 抗体 (A7L6)

sc-33707
200 µg/ml
RMB2377.00

Perlecan 抗体 (A7L6) AC

sc-33707 AC
500 µg/ml, 25% agarose
RMB3129.00

Perlecan 抗体 (A7L6) HRP

sc-33707 HRP
200 µg/ml
RMB2377.00

Perlecan 抗体 (A7L6) FITC

sc-33707 FITC
200 µg/ml
RMB2482.00

Perlecan 抗体 (A7L6) PE

sc-33707 PE
200 µg/ml
RMB2580.00

Perlecan 抗体 (A7L6) Alexa Fluor® 488

sc-33707 AF488
200 µg/ml
RMB2685.00

Perlecan 抗体 (A7L6) Alexa Fluor® 546

sc-33707 AF546
200 µg/ml
RMB2685.00

Perlecan 抗体 (A7L6) Alexa Fluor® 594

sc-33707 AF594
200 µg/ml
RMB2685.00

Perlecan 抗体 (A7L6) Alexa Fluor® 647

sc-33707 AF647
200 µg/ml
RMB2685.00

Perlecan 抗体 (A7L6) Alexa Fluor® 680

sc-33707 AF680
200 µg/ml
RMB2685.00

Perlecan 抗体 (A7L6) Alexa Fluor® 790

sc-33707 AF790
200 µg/ml
RMB2685.00

I would like to use Perlecan to perform IHC on mice brain tissue to tag Perlecan-positive neurons. However, it's hard to find a protocol for this. I would appreciate if you help me.

Asked by: Bilge
Thank you for contacting Santa Cruz Biotechnology. Here is our IHC protocol :https://www.scbt.com/resources/protocols/immunoperoxidase-staining .
Answered by: BlakeJ
Date published: 2025-02-25

I would like to use Perlecan to do immunoblot on mice kidney tissue, I would appreciate if someone here could share their protocol

Asked by: pochabei
Thank you for your question. This is the link to the protocol for WB: https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Technical Service SH
Date published: 2023-08-21

I would like to use Perlecan to stain spheroids of adipocytes, I would appreciate if someone here could share their protocol

Asked by: camila22
Thank you for your question. You can find our recommended protocol for Immunofluorescence Cell Staining by following the link below. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Support Europe
Date published: 2022-05-12

I would like to use Perlecan (A7L6): sc-33707 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-33707 is not available directly conjugated, but we have an alternative Perlecan antibody (sc-377219) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-26
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Rated 4 out of 5 by from Good for immunofluorescent stainingAntibody works well in immunofluorescence of a spheroid generated using low adherent culture plate.
Date published: 2017-10-31
Rated 5 out of 5 by from Great IF resultsNice IF results were produced with Perlecan Antibody (A7L6)
Date published: 2017-01-18
Rated 4 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for IF on pig aortic endothelial cells, medial menisci, and articular cartilage - SCBT Publication Review
Date published: 2015-05-02
Rated 4 out of 5 by from Successful application of this antibody inSuccessful application of this antibody in IHC (acetone) using mouse liver -SCBT Publication Review
Date published: 2015-04-08
Rated 4 out of 5 by from Produced great immunofluorescence basementProduced great immunofluorescence basement membrane, stroma and Descemet s membrane staining in normal mouse eye frozen section. -SCBT QC
Date published: 2015-02-01
Rated 4 out of 5 by from Publishable WB data with mouse brain tissuePublishable WB data with mouse brain tissue extracts - SCBT Publication Review
Date published: 2015-01-20
Rated 4 out of 5 by from IF publishable data with mouse spleenIF publishable data with mouse spleen - SCBT Publication Review
Date published: 2014-08-15
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Perlecan 抗体 (A7L6) is rated 4.1 out of 5 by 7.
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