Date published: 2025-9-7
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PERK Antibody (B-5) - Western Blotting - Image 350739
PERK 抗体 (B-5): sc-377400. 对 MCF7 (A), K-562 (B), SK-N-MC (C) 和 A549 (D) 全细胞裂解液中 PERK 表达的 Western 印迹分析.
PERK 抗体 (B-5): sc-377400. 对 MCF7 (A), K-562 (B), SK-N-MC (C) 和 A549 (D) 全细胞裂解液中 PERK 表达的 Western 印迹分析.

PERK 抗体 (B-5): sc-377400

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说明书
  • PERK 抗体 B-5 是小鼠单克隆 IgG1 κ，PERK抗体, 在112篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列21-320位于human物种的PERK的N-terminus附近
  • PERK 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PERK
  • 抗PERK抗体（B-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PERK (B-5): sc-377400的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 PERK 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PERK 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PERK抗体（B-5）是一种IgG1 κ小鼠单克隆PERK抗体（也被指定为EIF2AK3抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测来自小鼠、大鼠和人类的PERK蛋白。PERK抗体（B-5）既有非偶联的抗PERK抗体形式，也有多种偶联形式的抗PERK抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。已描述了一种干扰素可诱导的、依赖于RNA的蛋白丝氨酸/苏氨酸激酶（PKR）。在早期文献中，PKR对于小鼠激酶而言，有DAI、dsJ、PI激酶、p65、p67或TIK等多种名称；对于人类激酶而言，有p68或p69等名称。PKR激酶底物是蛋白质合成起始因子eIF-2的α亚基。eIF-2α在丝氨酸-51上的磷酸化导致翻译抑制。丝氨酸/苏氨酸激酶催化结构域映射到蛋白质的羧基末端一半，而RNA结合结构域位于氨基末端区域。PERK是一种位于内质网（ER）的I型跨膜蛋白，含有一个与PKR激酶结构域相似的激酶结构域。PERK在ER应激时被激活并磷酸化eIF-2α，从而抑制mRNA的翻译。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PERK 抗体 (B-5) 参考文献:

  1. 突变型胰腺 eIF-2alpha 激酶 PEK 的特征及在胰岛 delta 细胞中与体生长抑素的共定位。  |  Shi, Y., et al. 1999. J Biol Chem. 274: 5723-30. PMID: 10026192
  2. 干扰素的作用机制：人体细胞中干扰素诱导, RNA 依赖性 P1/eIF-2 alpha 蛋白激酶的 cDNA 结构, 表达和调节。  |  Thomis, DC., et al. 1992. Virology. 188: 33-46. PMID: 1373553
  3. 干扰素的作用机制：在人类 RNA 依赖性 P1/eIF-2 alpha 蛋白激酶的 N 端区域鉴定 RNA 结合域。  |  McCormack, SJ., et al. 1992. Virology. 188: 47-56. PMID: 1373554
  4. 干扰素诱导的人类双链 RNA 激活蛋白激酶的分子克隆和特征描述。  |  Meurs, E., et al. 1990. Cell. 62: 379-90. PMID: 1695551
  5. 蛋白质磷酸化控制着翻译速度。  |  Hershey, JW. 1989. J Biol Chem. 264: 20823-6. PMID: 2687263
  6. eIF-2 alpha 蛋白激酶是真核生物（从酵母到人类）翻译的调节器。  |  Samuel, CE. 1993. J Biol Chem. 268: 7603-6. PMID: 8096514
  7. 蛋白质的翻译和折叠是由内质网驻留激酶耦合完成的。  |  Harding, HP., et al. 1999. Nature. 397: 271-4. PMID: 9930704

订购信息

产品名称产品编号规格价格数量收藏夹

PERK 抗体 (B-5)

sc-377400
200 µg/ml
RMB2377.00

PERK (B-5): m-IgG Fc BP-HRP 套装

sc-530021
200 µg Ab; 10 µg BP
RMB2662.00

PERK (B-5): m-IgGκ BP-HRP 套装

sc-523313
200 µg Ab, 40 µg BP
RMB2662.00

PERK (B-5): m-IgG1 BP-HRP 套装

sc-543822
200 µg Ab; 20 µg BP
RMB2662.00

PERK 抗体 (B-5) AC

sc-377400 AC
500 µg/ml, 25% agarose
RMB3129.00

PERK 抗体 (B-5) HRP

sc-377400 HRP
200 µg/ml
RMB2377.00

PERK 抗体 (B-5) FITC

sc-377400 FITC
200 µg/ml
RMB2482.00

PERK 抗体 (B-5) PE

sc-377400 PE
200 µg/ml
RMB2580.00

PERK 抗体 (B-5) Alexa Fluor® 488

sc-377400 AF488
200 µg/ml
RMB2685.00

PERK 抗体 (B-5) Alexa Fluor® 546

sc-377400 AF546
200 µg/ml
RMB2685.00

PERK 抗体 (B-5) Alexa Fluor® 594

sc-377400 AF594
200 µg/ml
RMB2685.00

PERK 抗体 (B-5) Alexa Fluor® 647

sc-377400 AF647
200 µg/ml
RMB2685.00

PERK 抗体 (B-5) Alexa Fluor® 680

sc-377400 AF680
200 µg/ml
RMB2685.00

PERK 抗体 (B-5) Alexa Fluor® 790

sc-377400 AF790
200 µg/ml
RMB2685.00

Do you have immunofluorescence images of using bulk Anti-PERK Antibody (B-5): sc-377400?

Asked by: JoeD
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-06-27

Could you please send me the datasheet of the discontinued antibody sc-32577 for p-PERK? Thank you

Asked by: Ana Sayuri Yamagata
Thank you for your question. Here is the link to the datasheet: https://datasheets.scbt.com/sc-32577.pdf Since you also inquired about this one: https://datasheets.scbt.com/sc-571.pdf
Answered by: Tech Service
Date published: 2021-08-26

How do I get a free 100ug sample of PERK (B-5): sc-377400, as advertise above?

Asked by: mimin25
Thank you for your question. Please contact our technical service directly to arrange for a free 10 µg sample of PERK (B-5): sc-377400.
Answered by: Technical Support Europe
Date published: 2019-05-07

Do you have the colorimetric detection antibodies for PERK, ATF4, ATF6, CHOP, IRE-1, eIF2alpha

Asked by: mswestern
Thank you for the question. We provide many of our primary antibodies as direct conjugate. We offer many different conjugates including conjugation with enzymes and fluorochromes. If you can not find a desired conjugate on the corresponding product website, please contact us by email to scbt @ scbt.com to inquiry about the possibility of a special order.
Answered by: Technical Support Europe
Date published: 2017-12-27

I am working with mouse samples. How can I use a mouse monoclonal for IHC?

Asked by: Cweed
We recommend using a directly conjugated antibody. This product, sc-377400, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 9
Date published: 2017-01-20
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Rated 5 out of 5 by from works great for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:200 for overnight @ 4oC.
Date published: 2024-12-10
Rated 4 out of 5 by from Anti-PERK per analisi in western blotL'Ab è stato testato utilizzando un lisato cellulare di MDA-MD-231 ottenuto in RIPA buffer. Sono state utilizzate cellule controllo e cellule trattate con induttori dello stress del reticolo (tapsigargina, tunicanicina). 20 ug di proteine sono state separate tramite SDS-Page e trasferite su un filtro di nitrocellulosa. Il bloccaggio è stato effettuato in Blotto A per 1h, l'Ab primario è stato utilizzato alla diluizione di 1:1000 in 10 mL di Blotto A per 1h. Il secondario 1:2000 in Blotto A per 1h. Il segnale ottenuto è pulito, con un'unica banda del perso molecolare atteso. E' evidente la modulazione del segnale nelle cellule trattate con attivatori dello stress del reticolo.
Date published: 2024-07-23
Rated 4 out of 5 by from WBできます100倍希釈でウェスタンブロットを行った。バックグラウンドが少し高いが、HEK293で内在性のPERKをきちんと認識できた。
Date published: 2023-12-15
Rated 4 out of 5 by from Good work!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2023-01-20
Rated 5 out of 5 by from Great antibody-PERKThis antibody worked great for me on western blot.
Date published: 2021-12-03
Rated 3 out of 5 by from 非特異的バンドが検出される希釈倍率1:1000で使用したところ、PERKの特異的なバンドを確認することはできましたが、非特異的バンドが強く検出されます。
Date published: 2021-06-18
Rated 1 out of 5 by from did't workIt didn’t work for Western Blotting at the dilution of 1:500 overnight in the NB69 neuroblastoma cell line.
Date published: 2021-04-22
Rated 4 out of 5 by from western blottingWork well in WB (1:400) . breast cancer cell lysis
Date published: 2019-08-24
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PERK 抗体 (B-5) is rated 4.2 out of 5 by 46.
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