Date published: 2025-9-7
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CD31/PECAM-1 Antibody (MEC 13.3) - Western Blotting - Image 14722
CD31/PECAM-1 抗体 (MEC 13. 3): sc-18916. 非还原条件下小鼠 PBL (A) 和 CTLL-2 (B) 全细胞裂解液中 PECAM-1 表达的 Western 印迹分析.
CD31/PECAM-1 Antibody (MEC 13.3) - Western Blotting - Image 14722
CD31/PECAM-1 抗体 (MEC 13. 3): sc-18916. 非还原条件下小鼠 PBL (A) 和 CTLL-2 (B) 全细胞裂解液中 PECAM-1 表达的 Western 印迹分析.
CD31/PECAM-1 Antibody (MEC 13.3) - Flow Cytometry - Image 6023
CD31/PECAM-1 抗体 (MEC 13. 3) PE: sc-18916 PE. 小鼠外周血白细胞的 FCM 分析. 象限标记根据同型对照正常大鼠 IgG2a: sc-2872 设置.
CD31/PECAM-1 Antibody (MEC 13.3) - Immunofluorescence - Image 11415
CD31/PECAM-1 抗体 (MEC 13. 3): sc-18916. 甲醇固定的 WEHI-3 细胞免疫荧光染色, 显示膜定位.
CD31/PECAM-1 抗体 (MEC 13. 3): sc-18916. 非还原条件下小鼠 PBL (A) 和 CTLL-2 (B) 全细胞裂解液中 PECAM-1 表达的 Western 印迹分析.

CD31/PECAM-1 抗体 (MEC 13.3): sc-18916

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寫評論提問

说明书
  • CD31/PECAM-1 抗体 MEC 13.3 是大鼠单克隆 IgG2a, 在119篇文献中引用，提供 200 µg/ml
  • 针对129/Sv mouse衍生内皮瘤细胞系tEnd.1
  • 抗-CD31/PECAM-1 抗体 (MEC 13.3) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 PECAM-1
  • 抗CD31/PECAM-1抗体（MEC 13.3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供无叠氮化物形式sc-18916 L，200 µg/0.1 ml，用于体外和体内的黏附阻断
  • 关于如何获取CD31/PECAM-1 (MEC 13.3): sc-18916的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD31/PECAM-1 抗体 (MEC 13.3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PECAM 抗体

PECAM-1抗体（MEC 13.3）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）等方法检测小鼠、大鼠和人类样本中的PECAM-1。PECAM-1，也称为CD31，是一种重要的糖蛋白，在细胞间粘附中起着重要作用，特别是在血管系统中，PECAM-1主要在内皮细胞、血小板和某些白细胞表面表达。PECAM-1对于维持血管完整性和促进炎症反应中的白细胞迁移至关重要，因此PECAM-1在免疫功能和组织稳态中扮演着重要角色。PECAM-1还参与血管生成，即新血管的形成过程，这对于伤口愈合和组织修复至关重要。抗PECAM-1抗体（MEC 13.3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。研究人员利用PECAM-1单克隆抗体（MEC 13.3），可以深入了解细胞粘附和迁移的动态，这对于理解各种生理和病理过程至关重要，包括癌症转移和心血管疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD31/PECAM-1 抗体 (MEC 13.3) 参考文献:

  1. 通过内皮细胞中 Nrf2 介导的途径调节 PECAM-1 依赖性血红素加氧酶-1。  |  Saragih, H., et al. 2014. Thromb Haemost. 111: 1077-88. PMID: 24500083
  2. CD31（PECAM-1）是产气荚膜梭菌β毒素的内皮细胞特异性受体。  |  Bruggisser, J., et al. 2020. Cell Host Microbe. 28: 69-78.e6. PMID: 32497498
  3. 神经细胞粘附分子（NCAM）作为突触重塑的定量标记。  |  Jørgensen, OS. 1995. Neurochem Res. 20: 533-47. PMID: 7643959
  4. 细胞粘附受体与哺乳动物早期心脏发育：综述。  |  Buck, CA., et al. 1993. C R Acad Sci III. 316: 838-59. PMID: 8076214
  5. 先天性心脏病：手术三年。  |  McCue, CM., et al. 1977. Va Med. 104: 22-9. PMID: 831372
  6. NCAM使用的Vase小外显子并不局限于神经外胚层肿瘤。  |  Patel, K., et al. 1993. Int J Cancer. 54: 772-7. PMID: 8325706
  7. 精神分裂症的卡尔曼综合征变异型（KSV）模型。  |  Cowen, MA. and Green, M. 1993. Schizophr Res. 9: 1-10. PMID: 8461265
  8. 一名威尔姆斯局部肿瘤患者的骨髓微转移。  |  Dominici, C., et al. 1996. Med Pediatr Oncol. 26: 125-8. PMID: 8531850
  9. 粘膜血管地址素与其淋巴细胞受体α4β7结合的结构要求。整合素-Ig 家族相互作用的共同主题。  |  Briskin, MJ., et al. 1996. J Immunol. 156: 719-26. PMID: 8543825
  10. 血小板/内皮细胞粘附分子-1（PECAM-1，CD31）在自然杀伤细胞跨内皮迁移和 beta 2 整合素激活中的作用。  |  Berman, ME., et al. 1996. J Immunol. 156: 1515-24. PMID: 8568255
  11. Hox 和 Pax 基因产品对 N-CAM 表达的发育控制。  |  Edelman, GM. and Jones, FS. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 305-12. PMID: 8577842

订购信息

产品名称产品编号规格价格数量收藏夹

CD31/PECAM-1 抗体 (MEC 13.3)

sc-18916
200 µg/ml
RMB2377.00

CD31/PECAM-1 抗体 (MEC 13.3) L

sc-18916 L
200 µg/0.1 ml
RMB2377.00

CD31/PECAM-1 抗体 (MEC 13.3) AC

sc-18916 AC
500 µg/ml, 25% agarose
RMB3129.00

CD31/PECAM-1 抗体 (MEC 13.3) HRP

sc-18916 HRP
200 µg/ml
RMB2377.00

CD31/PECAM-1 抗体 (MEC 13.3) FITC

sc-18916 FITC
200 µg/ml
RMB2482.00

CD31/PECAM-1 抗体 (MEC 13.3) PE

sc-18916 PE
200 µg/ml
RMB2580.00

CD31/PECAM-1 抗体 (MEC 13.3) Alexa Fluor® 488

sc-18916 AF488
200 µg/ml
RMB2685.00

CD31/PECAM-1 抗体 (MEC 13.3) Alexa Fluor® 546

sc-18916 AF546
200 µg/ml
RMB2685.00

CD31/PECAM-1 抗体 (MEC 13.3) Alexa Fluor® 594

sc-18916 AF594
200 µg/ml
RMB2685.00

CD31/PECAM-1 抗体 (MEC 13.3) Alexa Fluor® 647

sc-18916 AF647
200 µg/ml
RMB2685.00

CD31/PECAM-1 抗体 (MEC 13.3) Alexa Fluor® 680

sc-18916 AF680
200 µg/ml
RMB2685.00

CD31/PECAM-1 抗体 (MEC 13.3) Alexa Fluor® 790

sc-18916 AF790
200 µg/ml
RMB2685.00

Has this antibody ever been used on Dynabeads to isolate mouse endothelial cells to your knowledge?

Asked by: Tasha
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-28

Cd31 pecam alexa  568 구매 가능 여부 문의 드립니다 

Asked by: Enyang7
Thank you for your interest in Santa Cruz Biotechnology products. Inquires for your region are best handled by our distributors in Korea. Please find contact information below. KOREA For ordering or product information contact us at: Essence Medical Inc. 417 Leaders B/D, Yatapdong 342-1 Bundanggu Seongnam Gyeonggi-do Korea Phone: 82-31-707-1544 Fax: 82-31-707-1999 Email: [email protected] Website: www.essencemedical.com Koram Biotech Corporation 6F, Koram Venture Building 907-1 Daechi-Dong Kang Nam-Ku Seoul 135-080 Korea Phone: 82 (2) 556-0311 Fax: 82 (2) 556-0828 Email: [email protected] Website: www.korambiotech.com
Answered by: Zhanglong
Date published: 2021-11-01

Dear technical support Team, can you please identify those references in which Mec13.3 was used in Western Blot assays? Thanks, Ruth

Asked by: rlyck
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Can I use PECAM-1 Antibody (MEC 13.3): sc-18916 to block PECAM-1 activity?

Asked by: jenni
Yes, we recommend using the azide-free form, sc-18916 L, for in vitro and in vivo studies for blocking PECAM-1 adhesion. The concentration provided of the azide-free form is 200 µg in 0.1 ml.
Answered by: Technical Support 2
Date published: 2024-12-13
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Rated 5 out of 5 by from 呈现的照片很漂亮在我们的实验中，切片免疫荧光成功应用了该抗体，呈现出清晰、特异的染色信号，背景干扰较低，能够准确标记目标蛋白，具有良好的重复性和可靠性，为后续的图像分析和定量研究提供了有力支持，表现出较高的实验适用性和科研价值。
Date published: 2025-04-12
Rated 5 out of 5 by from Buon funzionamentoAbbiamo testato quest' anticorpo in estratti da cellule di melanoma. Con una diluizione 1:1000 si ottiene un buon segnale con bande aspecifiche basse.
Date published: 2025-01-08
Rated 4 out of 5 by from 小鼠脑部切片染色CD31这款CD31/PECAM-1 抗体 (MEC 13.3)抗体功能还OK，但是感觉效价有点低，稀释倍数少一点，不要稀释太多，还是能染出来，祝好运
Date published: 2022-01-27
Rated 1 out of 5 by from Did not work on rat brainWe tested this antibody with a range of dilutions on frozen rat brain tissue fixed with methanol or 4% paraformaldehyde and observed no staining.
Date published: 2022-01-20
Rated 3 out of 5 by from western blotsc-18916を用いて、ウエスタンブロットを検討したところ、バンドを検出できました。少しバンドの見え方が薄かったです。
Date published: 2021-04-01
Rated 4 out of 5 by from Nice IFAt a 1:100 dilution, saw nice surface expression of PECAM-1 on human T cells.
Date published: 2017-11-20
Rated 4 out of 5 by from No cross-reactivity with chicken proteinsWe tested this antibody for cross-reactivity with chicken protein and no labelling could be seen (IF on frozen-sections). The company sent us a sample to test for free so this was pretty nice!
Date published: 2017-09-28
Rated 5 out of 5 by from successfulThe strong and clear band, I used it for WB in rat tissue whole lysate
Date published: 2017-09-06
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CD31/PECAM-1 抗体 (MEC 13.3) is rated 4.5 out of 5 by 19.
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