Date published: 2025-9-8
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PDH-E1α Antibody (D-6) - Western Blotting - Image 356589
PDH-E1α 抗体 (D-6): sc-377092. Hep G2 (A), HeLa (B), Sol8 (C), C2C12 (D), L8 (E) 和 L6 (F) 全细胞裂解液中 PDH-E1α 表达的 Western 印迹分析.
PDH-E1α 抗体 (D-6): sc-377092. Hep G2 (A), HeLa (B), Sol8 (C), C2C12 (D), L8 (E) 和 L6 (F) 全细胞裂解液中 PDH-E1α 表达的 Western 印迹分析.

PDH-E1α 抗体 (D-6): sc-377092

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说明书
  • PDH-E1 alpha 抗体 D-6 是小鼠单克隆 IgG2a κ，PDH-E1α抗体, 在79篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列31-161位于human物种的PDH-E1α的N-terminus附近
  • PDH-E1 alpha 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PDH-E1α; 也和以下物种反应，包括： 和 bovine and porcine
  • 抗PDH-E1 alpha抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PDH-E1α (D-6): sc-377092的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 PDH-E1α 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PDH-E1α 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PDH-E1 抗体

PDH-E1α抗体（D-6）是一种IgG2a κ小鼠单克隆PDH-E1α抗体（也称为ODPA抗体、丙酮酸脱氢酶E1亚基α1抗体、丙酮酸脱氢酶E1组分亚基α体细胞型线粒体抗体、丙酮酸脱氢酶（硫辛酸）α1抗体、丙酮酸脱氢酶α1抗体、PDHE1-A I型抗体、PHE1A抗体、PDHCE1A抗体、PDHAD抗体或PDHA抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的PDH-E1α蛋白。PDH-E1α抗体（D-6）既有非偶联的抗PDH-E1α抗体形式，也有多种偶联形式的抗PDH-E1α抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。丙酮酸脱氢酶（PDH）复合物是一种核编码的线粒体基质酶复合物，通过催化丙酮酸不可逆转化为乙酰辅酶A，在糖酵解和三羧酸（TCA）循环之间起主要连接作用。PDH复合物的E1酶由两个α和两个β亚基的异四聚体组成。E1-α亚基（PDH-E1α）含有E1活性位点，在PDH复合物的功能中发挥关键作用。PDH复合物受PDH-E1α的磷酸化和去磷酸化调节。编码PDH-E1α的基因位于Xp22-q24染色体上，该基因最后一个外显子中的20bp缺失足以引起PDH缺乏，导致一系列广泛的症状，包括癫痫发作、智力迟钝和痉挛的发展，以及与小脑共济失调相关的间歇性乳酸酸中毒发作。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PDH-E1α 抗体 (D-6) 参考文献:

  1. 一例患有严重代谢性乳酸酸中毒的男性患者的 PDH-E1 alpha 嵌合症。  |  Seyda, A., et al. 2001. J Inherit Metab Dis. 24: 551-9. PMID: 11757583
  2. 运动时人体骨骼肌中 PDH-E1alpha 的去磷酸化和激活：注入脂质的影响。  |  Pilegaard, H., et al. 2006. Diabetes. 55: 3020-7. PMID: 17065338
  3. 丙酮酸脱氢酶（PDH）E1 alpha 基因外显子 II 中的 20-bp 缺失导致丙酮酸脱氢酶缺乏症。  |  Chun, K., et al. 1991. Am J Hum Genet. 49: 414-20. PMID: 1907799
  4. 猪 PDH-E1 alpha cDNA 的特征及与人类 cDNA 的比较。  |  Sermon, K., et al. 1990. Nucleic Acids Res. 18: 4925. PMID: 2395657
  5. PI3K/Akt通路通过丙酮酸脱氢酶（PDH）-E1α磷酸化调节氧代谢。  |  Cerniglia, GJ., et al. 2015. Mol Cancer Ther. 14: 1928-38. PMID: 25995437
  6. MAPK信号传导诱导的PDHE1α磷酸化及亚细胞易位促进肿瘤免疫逃逸。  |  Zhang, Y., et al. 2022. Nat Metab. 4: 374-388. PMID: 35315437
  7. PARylated PDHE1α生成乙酰辅酶A，用于局部染色质乙酰化和DNA损伤修复。  |  Zhang, J., et al. 2023. Nat Struct Mol Biol. 30: 1719-1734. PMID: 37735618
  8. 丙酮酸脱氢酶缺乏症由 PDH E1 alpha 亚基中的 33 个碱基对重复引起。  |  Hansen, LL., et al. 1994. Hum Mol Genet. 3: 1021-2. PMID: 7545958
  9. 丙酮酸脱氢酶缺乏症。  |  Brown, GK., et al. 1994. J Med Genet. 31: 875-9. PMID: 7853374
  10. 丙酮酸脱氢酶 X 连锁 E1 α 亚基突变导致丙酮酸脱氢酶复合物缺乏。  |  Chun, K., et al. 1993. Hum Mol Genet. 2: 449-54. PMID: 8504306
  11. 哺乳动物 X 连锁和常染色体 Pgk 和 Pdh E1 alpha 亚基基因的进化。  |  Fitzgerald, J., et al. 1996. Mol Biol Evol. 13: 1023-31. PMID: 8752009
  12. 大脑发育不良和乳酸血症与女性 X 连锁 PDH E1 alpha 亚基突变的缔合作用。  |  Otero, LJ., et al. 1995. Pediatr Neurol. 13: 327-32. PMID: 8771169

订购信息

产品名称产品编号规格价格数量收藏夹

PDH-E1α 抗体 (D-6)

sc-377092
200 µg/ml
RMB2377.00

PDH-E1α (D-6): m-IgG Fc BP-HRP 套装

sc-529971
200 µg Ab; 10 µg BP
RMB2662.00

PDH-E1α (D-6): m-IgGκ BP-HRP 套装

sc-523183
200 µg Ab, 40 µg BP
RMB2662.00

PDH-E1α (D-6): m-IgG2a BP-HRP 套装

sc-547409
200 µg Ab; 10 µg BP
RMB2662.00

PDH-E1α 抗体 (D-6) AC

sc-377092 AC
500 µg/ml, 25% agarose
RMB3129.00

PDH-E1α 抗体 (D-6) HRP

sc-377092 HRP
200 µg/ml
RMB2377.00

PDH-E1α 抗体 (D-6) FITC

sc-377092 FITC
200 µg/ml
RMB2482.00

PDH-E1α 抗体 (D-6) PE

sc-377092 PE
200 µg/ml
RMB2580.00

PDH-E1α 抗体 (D-6) Alexa Fluor® 488

sc-377092 AF488
200 µg/ml
RMB2685.00

PDH-E1α 抗体 (D-6) Alexa Fluor® 546

sc-377092 AF546
200 µg/ml
RMB2685.00

PDH-E1α 抗体 (D-6) Alexa Fluor® 594

sc-377092 AF594
200 µg/ml
RMB2685.00

PDH-E1α 抗体 (D-6) Alexa Fluor® 647

sc-377092 AF647
200 µg/ml
RMB2685.00

PDH-E1α 抗体 (D-6) Alexa Fluor® 680

sc-377092 AF680
200 µg/ml
RMB2685.00

PDH-E1α 抗体 (D-6) Alexa Fluor® 790

sc-377092 AF790
200 µg/ml
RMB2685.00

PDH-E1α 抗体 (D-6) what is the meaning of D-6

Asked by: sunrise111
Thank you for your question. D-6 is the clone name of this primary antibody.
Answered by: Technical Service SH
Date published: 2023-03-16

How can PDH-E1α (D-6): sc-377092 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. PDH-E1α (D-6) is currently available conjugated to HRP (sc-377092 HRP), phycoerythrin (sc-377092 PE), fluorescein (sc-377092 FITC), Alexa Fluor® 488 (sc-377092 AF488) or Alexa Fluor® 647 (sc-377092 AF647)
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 4 out of 5 by from Works well for Western BlotThis antibody works well for detecting PDH in colorectal tumor samples.
Date published: 2022-02-17
Rated 4 out of 5 by from Good features.Immunohistochemical signal of free floating rat brain hippocampal section by DAB.
Date published: 2020-12-29
Rated 5 out of 5 by from ottimo segnaleHo utilizzato più volte questo anticorpo e ha dato sempre un ottimo segnale, molto chiaro e pulito.
Date published: 2020-10-06
Rated 4 out of 5 by from Works well for western blotReasonably strong band with minimal background using 20ug of lysate from mouse embryonic fibroblast cell line (MEF). Dilution 1:100, o/n incubation at 4C in 5% NFDM. Detected with Licor CW800 secondary using Odyssey imager.
Date published: 2019-10-01
Rated 4 out of 5 by from Performed wellWe used it on immunostaining and we obtained results comparable to previously published work.
Date published: 2018-01-23
Rated 5 out of 5 by from Great result for WBGreat result for western blot using NIH3T3L1 cells whole cell lysates. 1:1000.
Date published: 2017-08-21
Rated 5 out of 5 by from Great Antibody - PDH E1aI got this antibody sample, and it worked out perfectly with pig heart tissue samples with ration of 1:800, still very strong. for sample reason I could add photos, it said invalid photos... so I will just email the photos to technical support group.. thanks
Date published: 2017-08-17
Rated 4 out of 5 by from Great antibody for WBWorked perfectly with strongest band. Strongly recommended.
Date published: 2017-08-01
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PDH-E1α 抗体 (D-6) is rated 4.7 out of 5 by 15.
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