Date published: 2025-9-8

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PDGF-A 抗体 (A-1): sc-390392

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说明书
  • PDGF-A抗体(A-1)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PDGF-A的C-terminus的氨基酸182-211之间
  • 推荐用于 human 来源的precursor and mature PDGF-A WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP是PDGF-A Antibody (A-1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与PDGF-A Antibody (A-1) 搭配使用(请参阅下面的订购信息)。

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関連項目

血小板源性生长因子(PDGF)是间充质和神经胶质来源细胞的促有丝分裂原。它由两个二硫键连接的多肽链A和B组成,并以三种异构体形式存在,即PDGF AA、PDGF AB和PDGF BB。这三种异构体以不同的亲和力与两种受体类型α和β结合,这两种受体在结构上相关并赋有蛋白质酪氨酸激酶结构域。配体结合通过形成受体二聚体来诱导受体激酶的激活;PDGF的A亚基仅与高亲和力的α受体结合,而B亚基可以与α和β受体结合。有证据表明,PDGF可能起神经营养因子的作用。少突胶质细胞祖细胞表达DGF-A受体,而神经元表达PDGF-B受体,这表明PDGF的不同异构体可能通过旁分泌和自分泌途径调节发育中的中枢神经系统中不同细胞类型的生长和分化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PDGF-A 抗体 (A-1) 参考文献:

  1. 肺的生长和肺泡的形成需要PDGF-A/PDGF alpha受体信号,但早期肺分支形态发生不需要这种信号。  |  Boström, H., et al. 2002. Dev Dyn. 223: 155-62. PMID: 11803579
  2. 接受阿那格雷治疗的原发性血小板增多症患者的 PDGF-A、PDGF-B、TGFbeta 和 bFGF mRNA 水平。  |  Lev, PR., et al. 2005. Am J Hematol. 78: 155-7. PMID: 15682418
  3. Puralpha 通过与启动子中富含 G 的单链区域相互作用,激活 PDGF-A 基因转录。  |  Zhang, Q., et al. 2005. Gene. 348: 25-32. PMID: 15777709
  4. PDGF-A、-C 和 -D(而非 PDGF-B)会增加 TGF-beta1 和大鼠心脏同种异体移植物的慢性排斥反应。  |  Tuuminen, R., et al. 2009. Arterioscler Thromb Vasc Biol. 29: 691-8. PMID: 19213942
  5. PDGF-Ralpha 基因表达可预测新生小鼠肺肌成纤维细胞的增殖,但 PDGF-A 可抑制其转分化。  |  Kimani, PW., et al. 2009. Respir Res. 10: 119. PMID: 19939260
  6. 涎腺多形性腺瘤复发时 PDGF-A、PDGF-B 和 FGF-2 表达的增加。  |  Soares, AB., et al. 2012. J Clin Pathol. 65: 272-7. PMID: 22174424
  7. PDGF-A信号传导是继发性肺泡隔所必需的,并能控制肺发育过程中上皮细胞的增殖。  |  Gouveia, L., et al. 2018. Development. 145: PMID: 29636361
  8. PDGF-激活的 ERK 信号介导的 FAK-Paxillin 相互作用在少突胶质细胞祖细胞迁移中的作用  |  Singh, J., et al. 2019. J Mol Neurosci. 67: 564-573. PMID: 30649683
  9. 细胞外基质中 PDGF B 和细胞结合型 PDGF A 的积累。  |  Kelly, JL., et al. 1993. J Cell Biol. 121: 1153-63. PMID: 8501120
  10. 并非所有肌成纤维细胞都一样:利用 PDGF 靶向小鼠重新审视 PDGF-A 和 PDGF-B 的作用。  |  Lindahl, P. and Betsholtz, C. 1998. Curr Opin Nephrol Hypertens. 7: 21-6. PMID: 9442358

订购信息

产品名称产品编号规格价格数量收藏夹

PDGF-A 抗体 (A-1)

sc-390392
200 µg/ml
RMB2377.00

PDGF-A (A-1): m-IgG Fc BP-HRP 套装

sc-540796
200 µg Ab; 10 µg BP
RMB2662.00

PDGF-A (A-1) 中和勝肽

sc-390392 P
100 µg/0.5 ml
RMB511.00

what is the working concentration for this primary antibody?

Asked by: swapna
Thank you for your question. The recommended starting dilution for Western blot is 1:200 (range 1:100-1:1,000). Please refer to our product data sheet for recommendations for other applications.
Answered by: Technical Support
Date published: 2019-07-13

At what molecular weight should the band be seen in Western Blot with this antibody?

Asked by: TinTin
This antibody is reactive with both the precursor and mature form of PDGF-A. On Western blot, you may see it in different forms: as a monomeric A chain or as a dimer, or both.
Answered by: Technical Support 9
Date published: 2017-01-26
  • y_2025, m_8, d_30, h_9CST
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Rated 4 out of 5 by from pretty goodNeeded higher concentration to get band to show up - there are a few nonspecific bands but this is whole tissue lysate so it is pretty good overall
Date published: 2017-02-11
Rated 5 out of 5 by from Very good PDGF-A antibodyBeautiful immunofluorescence (IF) staining using PDGF-A (A-1) antibody in methanol-fixed HeLa cells.
Date published: 2017-01-28
Rated 5 out of 5 by from Superb immunofluorescence cytoplasmic andSuperb immunofluorescence cytoplasmic and membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Satisfactory Western Blot data of human recombinantSatisfactory Western Blot data of human recombinant PDGF-A fusion protein. -SCBT QC
Date published: 2013-08-17
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PDGF-A 抗体 (A-1) is rated 4.8 out of 5 by 4.
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