Date published: 2025-9-6

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PCAF 抗体 (E-8): sc-13124

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说明书
  • PCAF 抗体 E-8 是小鼠单克隆 IgG1 κ,PCAF抗体, 在111篇文献中引用,规格为200 µg/ml
  • 免疫human物种的p300/CBP-associated factor (PCAF)的氨基酸464-832
  • PCAF 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PCAF; 不会与 GCN5产生交联反应
  • 抗PCAF抗体(E-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PCAF (E-8): sc-13124免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 PCAF 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PCAF 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PCAF抗体(E-8)是一种小鼠单克隆IgG1 kappa轻链抗体,针对人类PCAF的氨基酸464-832,也称为P300/CBP相关因子、KAT2B或P/CAF。PCAF是一种组蛋白乙酰转移酶(HAT),通过乙酰化组蛋白上的赖氨酸残基来调节基因表达,从而起到关键作用,导致染色质重塑和转录激活。这种修饰会放松染色质结构,使转录因子更容易接近DNA,从而影响细胞周期进展、分化和对外部信号的反应等关键细胞过程。PCAF还会对非组蛋白蛋白进行乙酰化,影响其功能和稳定性,这凸显了它在各种细胞通路中的重要性。PCAF (E-8) 抗体特异性识别小鼠、大鼠和人源的 PCAF,不会与另一种功能相似的 HAT GCN5 发生交叉反应。PCAF (E-8) 单克隆抗体推荐用于蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA),是研究表观遗传调控和转录调控机制的研究人员必不可少的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PCAF 抗体 (E-8) 参考文献:

  1. PIN1通过增强m6A依赖性翻译促进METTL3稳定,从而促进乳腺癌的发生。  |  Wang, T., et al. 2018. J Cell Mol Med. 22: 5787-5800. PMID: 30216660
  2. 癌症主调控因子Pin1在癌症发展和治疗中的作用。  |  Koutelou, E., et al. 2021. Biochim Biophys Acta Gene Regul Mech. 1864: 194609. PMID: 32730897
  3. 通过PIN-FORMED 1和PIN1-GFP融合的突变抑制pinoid突变表型。  |  Ghasemi, A., et al. 2023. Clin Rheumatol. 42: 253-259. PMID: 36104638
  4. PIN1通过自噬保护听觉毛细胞免于衰老。  |  Ononye, OE. and Downey, M. 2022. PLoS Genet. 18: e1010352. PMID: 36107838
  5. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  6. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  7. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  8. 四膜虫组蛋白乙酰转移酶 A:酵母 Gcn5p 的同源物,将组蛋白乙酰化与基因激活联系起来。  |  Brownell, JE., et al. 1996. Cell. 84: 843-51. PMID: 8601308
  9. 一种与酵母转录调节因子 Rpd3p 相关的哺乳动物组蛋白去乙酰化酶。  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
  10. 一种与腺病毒癌蛋白 E1A 竞争的 p300/CBP 相关因子。  |  Yang, XJ., et al. 1996. Nature. 382: 319-24. PMID: 8684459
  11. YY1 对转录的抑制是通过与哺乳动物中酵母全局调节因子 RPD3 的同源物相互作用来介导的。  |  Yang, WM., et al. 1996. Proc Natl Acad Sci U S A. 93: 12845-50. PMID: 8917507
  12. 将小鼠组蛋白去乙酰化酶 1 鉴定为生长因子诱导基因  |  Bartl, S., et al. 1997. Mol Cell Biol. 17: 5033-43. PMID: 9271381

订购信息

产品名称产品编号规格价格数量收藏夹

PCAF 抗体 (E-8)

sc-13124
200 µg/ml
RMB2377.00

PCAF (E-8): m-IgG Fc BP-HRP 套装

sc-528263
200 µg Ab; 10 µg BP
RMB2662.00

PCAF (E-8): m-IgGκ BP-HRP 套装

sc-520596
200 µg Ab, 40 µg BP
RMB2662.00

PCAF (E-8): m-IgG1 BP-HRP 套装

sc-542846
200 µg Ab; 20 µg BP
RMB2662.00

PCAF 抗体 (E-8) AC

sc-13124 AC
500 µg/ml, 25% agarose
RMB3129.00

PCAF 抗体 (E-8) HRP

sc-13124 HRP
200 µg/ml
RMB2377.00

PCAF 抗体 (E-8) FITC

sc-13124 FITC
200 µg/ml
RMB2482.00

PCAF 抗体 (E-8) PE

sc-13124 PE
200 µg/ml
RMB2580.00

PCAF 抗体 (E-8) Alexa Fluor® 488

sc-13124 AF488
200 µg/ml
RMB2685.00

PCAF 抗体 (E-8) Alexa Fluor® 546

sc-13124 AF546
200 µg/ml
RMB2685.00

PCAF 抗体 (E-8) Alexa Fluor® 594

sc-13124 AF594
200 µg/ml
RMB2685.00

PCAF 抗体 (E-8) Alexa Fluor® 647

sc-13124 AF647
200 µg/ml
RMB2685.00

PCAF 抗体 (E-8) Alexa Fluor® 680

sc-13124 AF680
200 µg/ml
RMB2685.00

PCAF 抗体 (E-8) Alexa Fluor® 790

sc-13124 AF790
200 µg/ml
RMB2685.00

Can PCAF Antibody (E-8): sc-13124 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Yes, PCAF Antibody (E-8): sc-13124 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from Recommended for western blotting!It detected endogenous PCAF in lysates from HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween-20, overnight at 4C).
Date published: 2018-06-18
Rated 5 out of 5 by from Great specificity!The antibody to PCAF (E-8) is great to do Western blot, and the results show a very clear background.
Date published: 2018-02-25
Rated 5 out of 5 by from excellenttested on rat tissue samples in western blot and worked perfectly.
Date published: 2017-12-13
Rated 4 out of 5 by from good antibodyI received it as a sample product and it worked well.
Date published: 2017-07-25
Rated 5 out of 5 by from Overall great antibodyExcellent, well-published antibody for PCAF in mouse, rat, and human samples. Strong, specific signal observed in multiple applications.
Date published: 2017-01-16
Rated 5 out of 5 by from Great featuresI bought this antibody two months age, and I am very glad that this antibody is very efficient. I uesd PCAF of 1:500 dilution in Wb, second antibody is 1:4000. loaded ~50 ug. The band is clearly.
Date published: 2016-11-23
Rated 5 out of 5 by from Good specificity in WB in 293 cellsGood specificity in WB in 293 cells. -SCBT QC
Date published: 2015-05-31
Rated 5 out of 5 by from Strong pulldown in IP in human T3Strong pulldown in IP in human T3-1 cells. -SCBT Publication Review
Date published: 2015-05-19
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PCAF 抗体 (E-8) is rated 4.9 out of 5 by 12.
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