Date published: 2025-9-9
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PC-PLD1 Antibody (F-12) - Western Blotting - Image 304207
PC-PLD1 抗体 (F-12): sc-28314. MCF7 (A) 和 HeLa (B) 全细胞裂解液中 PC-PLD1 表达的 Western 印迹分析.
PC-PLD1 抗体 (F-12): sc-28314. MCF7 (A) 和 HeLa (B) 全细胞裂解液中 PC-PLD1 表达的 Western 印迹分析.

PC-PLD1 抗体 (F-12): sc-28314

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说明书
  • PC-PLD1 抗体 F-12 是小鼠单克隆 IgG2a κ，PC-PLD1抗体, 在18篇文献中引用，规格为200 µg/ml
  • 免疫human物种的PC-PLD1的氨基酸1-160
  • 推荐用于 mouse, rat 和 human 来源的PC-PLD1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取PC-PLD1 (F-12): sc-28314的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 PC-PLD1 抗体 (F-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PC-PLD1 抗体 (F-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PC-PLD 抗体 包括 PC-PLD2

PC-PLD1抗体（F-12）是一种IgG2a κ小鼠单克隆PC-PLD1抗体（也称为PC-PLD1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测来自小鼠、大鼠和人类的PC-PLD1蛋白。 PC-PLD1抗体（F-12）可以作为非偶联的抗PC-PLD1抗体使用。几乎每个细胞都使用磷脂酰胆碱作为底物来产生磷脂酸和胆碱。磷脂酰胆碱磷酸酯酶D1和D2（PC-PLD1和PC-PLD2）是特异性磷脂酶，可以水解磷脂酰胆碱。与PC-PLD1不同，PC-PLD2与分泌颗粒相关联，PC-PLD2定位于质膜，其参与细胞内吞作用的囊泡形成。 PC-PLD1和PC-PLD2协调调节巨噬细胞的吞噬作用。 G蛋白偶联受体和受体酪氨酸激酶细胞表面受体激活后，哺乳动物细胞中的PC-PLD活性会短暂激活。例如，PC-PLD1和PC-PLD2参与sphingosine 1-phosphate刺激支气管上皮细胞中ERK磷酸化和IL-8分泌。此外，结蛋白结合PC-PLD2可抑制muscarinic受体链接的PC-PLD2激活。 PC-PLD2还通过与脂肪酶活性无关的直接相互作用增强PKCzeta活性。 PC-PLD1和PC-PLD2通过p53依赖和p53独立的途径抑制p21基因的表达，从而促进细胞生长，最终可能导致癌变。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PC-PLD1 抗体 (F-12) 参考文献:

  1. 通过磷酸化依赖机制调节磷脂酶 D。  |  Houle, MG. and Bourgoin, S. 1999. Biochim Biophys Acta. 1439: 135-49. PMID: 10425391
  2. 哺乳动物磷脂酶 D1 和 D2 的信号作用。  |  Cockcroft, S. 2001. Cell Mol Life Sci. 58: 1674-87. PMID: 11706993
  3. 磷脂酶 D 同工酶合成乙酰胆碱所需的胆碱。  |  Zhao, D., et al. 2001. BMC Neurosci. 2: 16. PMID: 11734063
  4. 磷脂酶 D1 和 D2 参与 1-磷酸鞘氨醇诱导的 ERK（细胞外信号调节激酶）激活和人支气管上皮细胞白细胞介素-8 的分泌。  |  Wang, L., et al. 2002. Biochem J. 367: 751-60. PMID: 12149127
  5. 丝氨酸/苏氨酸蛋白激酶协同调节磷脂酶 D1 和 2 以及 RBL-2H3 肥大细胞的分泌。  |  Chahdi, A., et al. 2002. Mol Immunol. 38: 1269-76. PMID: 12217394
  6. 磷脂酶 D 和 c-Src 之间的传递调节可促进细胞增殖。  |  Ahn, BH., et al. 2003. Mol Cell Biol. 23: 3103-15. PMID: 12697812
  7. 磷脂酶 D1 和 D2 对细胞周期蛋白依赖性激酶抑制剂 p21 基因的转录抑制。  |  Kwun, HJ., et al. 2003. FEBS Lett. 544: 38-44. PMID: 12782287
  8. 磷脂酶 D1 和 D2 协调调节巨噬细胞的吞噬作用。  |  Iyer, SS., et al. 2004. J Immunol. 173: 2615-23. PMID: 15294978
  9. 磷脂酶 D2-Phox 同源结构域在蛋白激酶 Czeta 激活过程中的新功能。  |  Kim, JH., et al. 2005. Mol Cell Biol. 25: 3194-208. PMID: 15798205
  10. 大鼠脑缺血会降低磷脂酰胆碱-磷脂酶 D，但不会降低磷脂酰肌醇-磷脂酶 C。  |  Nishida, A., et al. 1994. Stroke. 25: 1247-51. PMID: 8202988
  11. ras蛋白对磷脂酶D的激活与蛋白激酶C无关。  |  del Peso, L., et al. 1996. J Cell Biochem. 61: 599-608. PMID: 8806084

订购信息

产品名称产品编号规格价格数量收藏夹

PC-PLD1 抗体 (F-12)

sc-28314
200 µg/ml
RMB2377.00

PC-PLD1 (F-12): m-IgG Fc BP-HRP 套装

sc-528418
200 µg Ab; 10 µg BP
RMB2662.00

PC-PLD1 (F-12): m-IgGκ BP-HRP 套装

sc-520792
200 µg Ab, 40 µg BP
RMB2662.00

PC-PLD1 (F-12): m-IgG2a BP-HRP 套装

sc-547091
200 µg Ab; 10 µg BP
RMB2662.00

I have used this antibody to detect PLD1 in A549 cells for WB, but the results (3 experiments) consistently are at about 40KD. What should I do?

Asked by: Melbrane
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-08-24

Can this antibody detect PC-PLD1 of mouse origin?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service
Date published: 2017-02-03
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Rated 4 out of 5 by from Worked for WB but not for IPThis antibody generates a very clean band on Western blot. However, it did not work for IP in mouse cells in our hands.
Date published: 2017-03-21
Rated 5 out of 5 by from Great for Western blottingPublished antibody great for detecting human PC-PLD1. Strong Western blot results, and good staining in IHC(P). Great for IP, IF, and ELISA as well.
Date published: 2017-01-30
Rated 4 out of 5 by from Good signal with this antibody in our WesternGood signal with this antibody in our Western.
Date published: 2015-06-09
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using MCF7 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-06-03
Rated 5 out of 5 by from Band detected at expected mw in UBand detected at expected mw in U-937 + LPS and MCF7 + insulin whole cell lysates. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Published data for WB in human leukocyte and HLPublished data for WB in human leukocyte and HL-60 cells. -SCBT Publication Review
Date published: 2015-04-28
Rated 5 out of 5 by from Worked perfectlyWorked perfectly.
Date published: 2015-04-08
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lower stomach tissue. -SCBT QC
Date published: 2015-03-13
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PC-PLD1 抗体 (F-12) is rated 4.8 out of 5 by 11.
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