Date published: 2025-9-8

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Pax-7 抗体 (EE-8): sc-81975

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说明书
  • Pax-7抗体(EE-8)是小鼠单克隆IgG2a κ, 在21篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组Pax-7
  • 推荐用于 mouse, rat 和 human 来源的Pax-7 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 Pax (D-7): sc-514352,可查看有标记的 Pax-7 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取Pax-7 (EE-8): sc-81975免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Pax-7 抗体 (EE-8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Pax-7抗体(EE-8)是一种小鼠单克隆IgG2a kappa轻链抗体,用于检测Pax-7蛋白,又称PAX7,是配对盒(PAX)家族核转录因子的重要成员。Pax-7 单克隆抗体(EE-8)针对重组人 Pax-7 产生,对鼠、大鼠和人源 Pax-7 具有强反应性,因此抗 Pax-7 抗体(EE-8)适用于多种应用,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)。Pax-7主要位于卫星细胞和肌细胞的细胞核中,在调节基因表达方面发挥着重要作用,而基因表达对于肌肉组织的分化与维持至关重要。Pax-7在细胞核中的精确定位对于其控制肌肉干细胞增殖和自我更新的功能至关重要,而肌肉干细胞的增殖和自我更新对于肌肉再生和修复非常重要。此外,Pax-7还参与多种信号转导通路,影响胚胎发育和成年生物体内的细胞分化。PAX7基因的突变和染色体畸变与多种疾病有关,包括2型横纹肌肉瘤(RMS2),也称为肺泡横纹肌肉瘤,这凸显了Pax-7在癌症研究中的重要性。Pax-7 (EE-8)抗体以非结合形式提供,为研究人员提供了可靠的工具,用于研究Pax-7在肌肉生物学和相关病理条件下的表达、定位和功能作用。通过靶向这一关键的转录因子,科学家可以更深入地了解控制肌肉发育、再生和肌肉相关疾病进展的分子机制。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Pax-7 抗体 (EE-8)

sc-81975
100 µg/ml
RMB2504.00

I am going to use the antibody for immunofluorescence in C2C12 cells. Which dilution should I use?

Asked by: IrisUM
Thank you for your question! For Immunofluorescence the dilution range is 1:50-1:500. We recommend a starting dilution of 1:50.
Answered by: Tech Support Europe
Date published: 2021-07-26

Qué pH se recomienda para la recuperación antigenica?

Asked by: tecAP
Gracias por su pregunta. Puede encontrar nuestro protocolo recomendado con métodos de recuperación de antígenos en este enlace: https://www.scbt.com/scbt/es/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2021-07-23

Can this antibody be used with equine samples?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 1
Date published: 2019-06-08

What is the recommended secondary for this primary antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 1
Date published: 2017-01-13
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Rated 5 out of 5 by from Very good signal by WB (1/1000) on RD cell lysateヒト横紋筋肉腫細胞株のタンパク質抽出物できれいなバンドが検出できました。抗体の希釈は、1000倍、ロードしたタンパク質量は20μグラム/lane。
Date published: 2022-12-27
Rated 1 out of 5 by from Not good for IHCIt did not work for IHC on hindlimbs during rat development. We tested it using several concentrations and several secondary antibodies.
Date published: 2021-04-05
Rated 5 out of 5 by from Good for IHCThis antibody works for IHC, showing strong specific staining.
Date published: 2017-01-12
Rated 5 out of 5 by from Very good signal by WB (1/500) on mouse andVery good signal by WB (1/500) on mouse and human skeletal muscle
Date published: 2016-04-11
Rated 5 out of 5 by from CleanClean, strong band seen in WB using NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Beautiful immunoperoxidase nuclear stainingBeautiful immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-02-22
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Pax-7 抗体 (EE-8) is rated 4.3 out of 5 by 6.
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