Date published: 2025-9-6
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PAK4 Antibody (B-3) - Western Blotting - Image 361646
PAK4 抗体 (B-3): sc-390507. NTERA-2 cl. D1 (A), SW480 (B), MCF7 (C), Jurkat (D) 和 HeLa (E) 全细胞裂解液中 PAK4 表达的 Western 印迹分析.
PAK4 抗体 (B-3): sc-390507. NTERA-2 cl. D1 (A), SW480 (B), MCF7 (C), Jurkat (D) 和 HeLa (E) 全细胞裂解液中 PAK4 表达的 Western 印迹分析.

PAK4 抗体 (B-3): sc-390507

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说明书
  • PAK4 抗体 B-3 是小鼠单克隆 IgG1 κ，PAK4抗体, 在12篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PAK4的内部区域内的氨基酸167-209之间
  • PAK4 抗体 (B-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PAK4
  • 抗PAK4抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PAK4 (B-3): sc-390507的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 PAK4 抗体 (B-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PAK4 抗体 (B-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PAK 抗体包括 和

PAK4抗体（B-3）是一种IgG1 κ小鼠单克隆PAK4抗体（也称为P21（RAC1）活化激酶4抗体、P21蛋白（Cdc42/Rac）活化激酶4抗体、丝氨酸/苏氨酸蛋白激酶PAK 4抗体、P21（CDKN1A）活化激酶4抗体、与S. Cerevisiae STE20效应物相关的蛋白激酶针对Cdc42Hs的抗体或P21活化激酶4抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的PAK4蛋白。PAK4抗体（B-3）既可以是非偶联的抗PAK4抗体形式，也可以是多种偶联形式的抗PAK4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。最近鉴定的三种丝氨酸/苏氨酸激酶同工型，称为αPAK p68、βPAK p65和γPAK p62，已显示出与S. cerevisiae激酶STE20具有高度序列同源性，后者参与信息素信号传导。α、β和γPAK同工型在其活性GTP结合状态下特异性地与Rac1和Cdc42复合，抑制它们的内在GTP酶活性并导致其自磷酸化。一旦磷酸化且它们对Rac/Cdc42的亲和力降低，PAK同工型就会从复合物中解离出来以寻找下游底物。其中一个这样的假定底物是MEK激酶，它是参与JNK信号通路的MEK4的上游效应器。虽然PAK同工型以GTP依赖的方式与Rac1和Cdc42相互作用，但它们不与Rho相互作用。PAK4在前列腺、睾丸和结肠中高度表达。PAK4与GTP结合的CDC42紧密结合，而不与GDP结合的CDC42结合，并且与RAC弱结合。PAK4使自身和其他底物磷酸化，并激活JNK通路。PAK4和活化的CDC42的共同表达诱导了富含肌动蛋白的丝状伪足的持续形成，并使PAK4与聚合的肌动蛋白簇和高尔基体中的beta coat蛋白共定位。编码PAK4的基因定位于人类19号染色体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PAK4 抗体 (B-3) 参考文献:

  1. 人胎盘 GTP 结合蛋白 Gp（G25K）基因的分子克隆：确定该 GTP 结合蛋白为酵母细胞分裂周期蛋白 CDC42 的人类同源物。  |  Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
  2. 癌症中微小 RNA 对 KRAS-PAK4 轴的调控。  |  Choudhry, ZS., et al. 2014. Curr Pharm Des. 20: 5275-8. PMID: 24479809
  3. rac，一种与 ras 相关的新型蛋白质家族，是肉毒毒素的底物。  |  Didsbury, J., et al. 1989. J Biol Chem. 264: 16378-82. PMID: 2674130
  4. PAK4 在免疫系统中的作用及其对癌症免疫疗法的潜在影响。  |  Naїja, A., et al. 2021. Cell Immunol. 367: 104408. PMID: 34246086
  5. 通过调节ER应激，靶向PAK4可逆转NSCLC的顺铂耐药性。  |  Liu, S., et al. 2024. Cell Death Discov. 10: 36. PMID: 38238316
  6. 小 GTP 结合蛋白 Rac1 和 Cdc42 可调节 JNK/SAPK 信号通路的活性。  |  Coso, OA., et al. 1995. Cell. 81: 1137-46. PMID: 7600581
  7. 一种由 rac1/CDC42Hs 依赖性自身磷酸化激活的新型丝氨酸激酶与 PAK65 和 STE20 有关。  |  Martin, GA., et al. 1995. EMBO J. 14: 1970-8. PMID: 7744004
  8. MEKK1 磷酸化活化剂 SEK1 激活应激活化蛋白激酶。  |  Yan, M., et al. Nature. 372: 798-800. PMID: 7997270
  9. 一种由 Cdc42 和 Rac1 激活的脑丝氨酸/苏氨酸蛋白激酶。  |  Manser, E., et al. 1994. Nature. 367: 40-6. PMID: 8107774
  10. 调节 Ras 及其近亲的蛋白质。  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
  11. 由 MEK 激酶和 Raf 定义的 MAP 激酶调控网络存在分歧。  |  Lange-Carter, CA., et al. 1993. Science. 260: 315-9. PMID: 8385802
  12. PAK4是Cdc42Hs的一种新型效应子，它与肌动蛋白细胞骨架的重组和丝状体的形成有关。  |  Abo, A., et al. 1998. EMBO J. 17: 6527-40. PMID: 9822598

订购信息

产品名称产品编号规格价格数量收藏夹

PAK4 抗体 (B-3)

sc-390507
200 µg/ml
RMB2377.00

PAK4 (B-3): m-IgG Fc BP-HRP 套装

sc-527637
200 µg Ab; 10 µg BP
RMB2662.00

PAK4 (B-3): m-IgG1 BP-HRP 套装

sc-533010
200 µg Ab; 20 µg BP
RMB2662.00

PAK4 抗体 (B-3) AC

sc-390507 AC
500 µg/ml, 25% agarose
RMB3129.00

PAK4 抗体 (B-3) HRP

sc-390507 HRP
200 µg/ml
RMB2377.00

PAK4 抗体 (B-3) FITC

sc-390507 FITC
200 µg/ml
RMB2482.00

PAK4 抗体 (B-3) PE

sc-390507 PE
200 µg/ml
RMB2580.00

PAK4 抗体 (B-3) Alexa Fluor® 488

sc-390507 AF488
200 µg/ml
RMB2685.00

PAK4 抗体 (B-3) Alexa Fluor® 546

sc-390507 AF546
200 µg/ml
RMB2685.00

PAK4 抗体 (B-3) Alexa Fluor® 594

sc-390507 AF594
200 µg/ml
RMB2685.00

PAK4 抗体 (B-3) Alexa Fluor® 647

sc-390507 AF647
200 µg/ml
RMB2685.00

PAK4 抗体 (B-3) Alexa Fluor® 680

sc-390507 AF680
200 µg/ml
RMB2685.00

PAK4 抗体 (B-3) Alexa Fluor® 790

sc-390507 AF790
200 µg/ml
RMB2685.00

PAK4 (B-3) 中和勝肽

sc-390507 P
100 µg/0.5 ml
RMB511.00

Which amount of cell lysate do you recommend for Western Blott for each lane, to see good results?

Asked by: AnTake
Thank you for your question. For Western Blotting we recommend a starting dilution of 1:100, with a dilution range of 1:100-1:1000. Optimum conditions for your particular experimental setup and samples will have to be determined experimentally.
Answered by: Tech Support Europe
Date published: 2023-01-26

What application is the blocking peptide sc-390507 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 2 out of 5 by from Weak with backgroundUsed a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. The signal was weak with some background. Blocked with milk.
Date published: 2017-11-30
Rated 4 out of 5 by from The band is a little weak1:200 diluted for wb of rat protein which extracted from h9c2 cells,the band is a little weak.
Date published: 2017-10-20
Rated 5 out of 5 by from My drug-resistant cells are significantly differenI diluted the ratio of 1:50, WB showed a good trend, I hope the follow-up experiment smoothly
Date published: 2017-09-04
Rated 5 out of 5 by from Clean and specific results!This PAK4 antibodies gives clean and specific results in Western blotting and immunohistochemistry applications.
Date published: 2017-02-02
Rated 4 out of 5 by from Potent and quite specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~68 kDa target protein in a HeLa/Jurkat cell lysate mix, and possibly in mouse tissues (brain/testes/thymus/muscle mix), where a ~80 kDa protein was observed that was about 12 kDa higher than predicted. No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for weak cross-reactivity with a ~20 kDa protein. A ~22 kDa strong cross-reactive protein was also evident in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-05-21
Rated 5 out of 5 by from WB: Worked well with human PAK4 transfectedWB: Worked well with human PAK4 transfected lysate. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Great antibody data in NTERAGreat antibody data in NTERA-2 cl.D1, SH-SY5Y and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-02-12
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PAK4 抗体 (B-3) is rated 4.4 out of 5 by 8.
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