Date published: 2025-9-6

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Paip1 抗体 (E-7): sc-365687

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说明书
  • Paip1 抗体 E-7 是小鼠单克隆 IgG2b κ,Paip1抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列180-479位于human物种的Paip1的C-terminus
  • Paip1 抗体 (E-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Paip1
  • 抗Paip1抗体(E-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Paip1 (E-7): sc-365687免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Paip1 抗体 (E-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Paip1 抗体 (E-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Paip1抗体(E-7)是一种IgG2b κ小鼠单克隆Paip1抗体(也被指定为Paip1抗体),它可以通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类的Paip1蛋白。Paip1抗体(E-7)既可以以非偶联的抗Paip1抗体形式存在,也可以以多种偶联形式的抗Paip1抗体存在,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。PAIP,即PABP相互作用蛋白,与多聚腺苷酸结合蛋白(PABP)结合,在酵母和植物中已显示其与真核起始因子组分eIF4G结合。存在两种称为Paip1和Paip2的Paip蛋白。Paip1刺激翻译,而Paip2与Paip1竞争与PABP结合,抑制翻译。Paip2降低了PABP对多聚腺苷酸RNA的亲和力,并破坏了多聚(A)核糖核蛋白的重复结构。Paip1包含一个eIF4A结合区和一个富含脯氨酸的N端。在COS-7细胞中过表达Paip1可以刺激翻译,可能是通过提供mRNA末端之间的物理联系来实现的。人类Paip1基因编码一个由480个氨基酸组成的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Paip1 抗体 (E-7) 参考文献:

  1. 多聚(A)结合蛋白的多个部分刺激体内翻译。  |  Gray, NK., et al. 2000. EMBO J. 19: 4723-33. PMID: 10970864
  2. 翻译耦合 mRNA 翻转的机制:poly(A) 尾部与 c-fos RNA 编码决定因子通过蛋白质复合物相互作用。  |  Grosset, C., et al. 2000. Cell. 103: 29-40. PMID: 11051545
  3. 人类多聚(A)结合蛋白 C 端 PABC 结构域的结构和功能。  |  Kozlov, G., et al. 2001. Proc Natl Acad Sci U S A. 98: 4409-13. PMID: 11287632
  4. 翻译抑制因子 Paip2 与聚(A)结合蛋白的双重相互作用。  |  Khaleghpour, K., et al. 2001. Mol Cell Biol. 21: 5200-13. PMID: 11438674
  5. 果糖调节分化的 Caco-2 细胞中 GLUT5 mRNA 的稳定性:cAMP 信号通路和 PABP(多腺苷酸结合蛋白)-互作蛋白(Paip)2 的作用。  |  Gouyon, F., et al. 2003. Biochem J. 375: 167-74. PMID: 12820898
  6. 多聚腺苷酸结合蛋白与 eIF4G 同源物 PAIP 的相互作用可促进翻译。  |  Craig, AW., et al. 1998. Nature. 392: 520-3. PMID: 9548260

订购信息

产品名称产品编号规格价格数量收藏夹

Paip1 抗体 (E-7)

sc-365687
200 µg/ml
$316.00

Paip1 (E-7): m-IgGκ BP-HRP 套装

sc-522330
200 µg Ab, 40 µg BP
$354.00

Paip1 (E-7): m-IgG2b BP-HRP 套装

sc-549021
200 µg Ab; 10 µg BP
$354.00

Paip1 抗体 (E-7) AC

sc-365687 AC
500 µg/ml, 25% agarose
$416.00

Paip1 抗体 (E-7) HRP

sc-365687 HRP
200 µg/ml
$316.00

Paip1 抗体 (E-7) FITC

sc-365687 FITC
200 µg/ml
$330.00

Paip1 抗体 (E-7) PE

sc-365687 PE
200 µg/ml
$343.00

Paip1 抗体 (E-7) Alexa Fluor® 488

sc-365687 AF488
200 µg/ml
$357.00

Paip1 抗体 (E-7) Alexa Fluor® 546

sc-365687 AF546
200 µg/ml
$357.00

Paip1 抗体 (E-7) Alexa Fluor® 594

sc-365687 AF594
200 µg/ml
$357.00

Paip1 抗体 (E-7) Alexa Fluor® 647

sc-365687 AF647
200 µg/ml
$357.00

Paip1 抗体 (E-7) Alexa Fluor® 680

sc-365687 AF680
200 µg/ml
$357.00

Paip1 抗体 (E-7) Alexa Fluor® 790

sc-365687 AF790
200 µg/ml
$357.00

Thank you for your suggestion! Based on viewing of the human protein atlas the salivary gland and thyroid gland express the PAIP1 protein! So can either of the two tissues be used as a positive control? Also do endothelial cells show positivity for PAIP1?

Asked by: Neeti Swarup
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Thank you for suggestion! I referred the atlas and found thyroid and salivary gland express PAIP1 protein, so can either of the two be used as an alternative to adrenal gland? Also can endothelial cells express PAIP1?

Asked by: Neeti Swarup
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

I want to use this antibody for IHC, can you please suggest the positive and negative controls to be used with this antibody for validation?

Asked by: Neeti
Thank you for your question. We recommend using human adrenal gland tissue as a positive control for Paip1 with this antibody. We have not characterized negative control tissues for Paip1. According to the Human Protein Atlas (HPA) PAIP1 has RNA detected in all tissues tested with low tissue specificity. Please refer to the link below for this reference. https://www.proteinatlas.org/ENSG00000172239-PAIP1/tissue
Answered by: Technical Support
Date published: 2020-08-04

Can Paip1 (E-7): sc-365687 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, Paip1 (E-7): sc-365687 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2020-08-04
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Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Nice Western Blot data of Paip1 expression in nonNice Western Blot data of Paip1 expression in non-transfected and mouse Paip1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-11-10
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Paip1 抗体 (E-7) is rated 5.0 out of 5 by 2.
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