Date published: 2025-9-6
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PAH Antibody (E-8) - Western Blotting - Image 131190
PAH 抗体 (E-8): sc-271257. 非转染: sc-117752 (A) 和小鼠 PAH 转染: sc-122353 (B) 293T 全细胞裂解液中 PAH 表达的 Western 印迹分析.
PAH Antibody (E-8) - Western Blotting - Image 131190
PAH 抗体 (E-8): sc-271257. 非转染: sc-117752 (A) 和小鼠 PAH 转染: sc-122353 (B) 293T 全细胞裂解液中 PAH 表达的 Western 印迹分析.
PAH Antibody (E-8) - Western Blotting - Image 412188
PAH抗体（E-8）：sc-271257。Hep G2全细胞裂解液中PAH表达的Western blot分析。所用检测试剂：m-IgG2a BP-HRP：sc-542731。
PAH 抗体 (E-8): sc-271257. 非转染: sc-117752 (A) 和小鼠 PAH 转染: sc-122353 (B) 293T 全细胞裂解液中 PAH 表达的 Western 印迹分析.

PAH 抗体 (E-8): sc-271257

5.0(1)
寫評論提問

说明书
  • PAH抗体(E-8)是小鼠单克隆IgG2a (kappa轻链)，规格为200 µg/ml
  • 特异性抗原位于human物种的PAH的C-terminus附近的氨基酸404-432之间
  • 推荐用于 mouse, rat 和 human 来源的PAH WB, IP, IF 和 ELISA检测; 也和以下物种反应，包括： 和 equine, canine and porcine
  • 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是PAH Antibody (E-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PAH Antibody (E-8) 打包提供（请参阅下面的订购信息）。

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    描述
    基因信息
    说明书与实验方案
    研究信息

    関連項目

    PAH基因编码苯丙氨酸羟化酶（PAH），该酶将苯丙氨酸转化为酪氨酸，是苯丙氨酸分解代谢中的限速酶。哺乳动物PAH是一种可溶性、同源四聚体蛋白质，在人类肝脏中大量表达。PAH活性缺陷导致常染色体隐性遗传病苯丙酮尿症（PKU），其特征为智力迟钝，除非在生命早期就引入低苯丙氨酸饮食。PAH基因位于人类染色体12q23.2上，包含编码功能性PAH所需的所有遗传信息，这表明单个基因参与了经典疾病表型。许多突变会损害PAH基因，导致酶活性降低并引起不同程度的PKU。据报道，PAH存在多种同工酶，但这些很可能是PAH的等位基因变异体，产生的蛋白质亚基电荷和电泳迁移率略有不同。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    PAH 抗体 (E-8)

    sc-271257
    200 µg/ml
    		RMB2377.00

    PAH (E-8): m-IgGκ BP-HRP 套装

    sc-534909
    200 µg Ab; 40 µg BP
    		RMB2662.00

    PAH (E-8): m-IgG2a BP-HRP 套装

    sc-546200
    200 µg Ab; 10 µg BP
    		RMB2662.00

    PAH (E-8) 中和勝肽

    sc-271257 P
    100 µg/0.5 ml
    		RMB511.00

    Dear SC tgeam, what are the difference between E-8 antibody and H2 antibody. They both target the same region in the C-terminal of PAH protein. Are they have a different epitopes ?

    Asked by: Dima
    Thank you for your question. These are different antibody clones that were raised against the same protein sequence but will have (minimally) different binding properties and may have different epitopes within the epitope region. Note also that these antibodies are different isotypes, i.e. IgG1 and IgG2a, respectively.
    Answered by: Tech Support Europe
    Date published: 2024-08-13

    Dear SC team, what are the difference between E-8 antibody and H2 antibody. They both target the same region in the C-terminal of PAH protein. Are they have a different epitopes ?

    Asked by: Dima
    Thank you for your question. These are different antibody clones that were raised against the same protein sequence but will have (minimally) different binding properties and may have different epitopes within the epitope region. Note also that these antibodies are different isotypes, i.e. IgG1 and IgG2a, respectively.
    Answered by: Tech Support Europe
    Date published: 2024-08-13

    What application is the blocking peptide sc-271257 P appropriate for?

    Asked by: Randy McDonald
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Satisfactory Western blot data of PAH expressionSatisfactory Western blot data of PAH expression in non-transfected and mouse PAH transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-04-17
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    PAH 抗体 (E-8) is rated 5.0 out of 5 by 1.
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