Date published: 2025-9-8
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pADPr Antibody (10H) - Immunofluorescence - Image 306512
pADPr 抗体 (10H): sc-56198. 福尔马林固定的 HeLa 细胞免疫荧光染色显示核定位. 波士顿儿童医院新生儿医学科, 哈佛医学院细胞生物学系向阳博士友情提供.
pADPr Antibody (10H) - Immunofluorescence - Image 306512
pADPr 抗体 (10H): sc-56198. 福尔马林固定的 HeLa 细胞免疫荧光染色显示核定位. 波士顿儿童医院新生儿医学科, 哈佛医学院细胞生物学系向阳博士友情提供.
pADPr 抗体 (10H): sc-56198. 福尔马林固定的 HeLa 细胞免疫荧光染色显示核定位. 波士顿儿童医院新生儿医学科, 哈佛医学院细胞生物学系向阳博士友情提供.

pADPr 抗体 (10H): sc-56198

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说明书
  • pADPr 抗体 10H 是小鼠单克隆 IgG3，pADPr抗体, 在68篇文献中引用，规格为100 µg/ml
  • 与甲基化牛血清白蛋白混合的聚（ADP-核糖）
  • 推荐用于 poly(ADP-ribose) polymer (pADPr) synthesized by a variety of pADPr polymerase (PARP)-related enzymes including PARP-1, -2, -3, tankyrase, vPARP, sPARP and others. WB, IF 和 ELISA检测; 可能与 bovine serum albumin产生交联反应; 不会与 ADP-ribose, 5′-AMP, or yeast RNA产生交联反应
  • 关于如何获取pADPr (10H): sc-56198的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是pADPr Antibody (10H) 适用于 WB 应用。 的首选辅助检测试剂，该试剂现与pADPr Antibody (10H) 搭配使用（请参阅下面的订购信息）。

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研究信息

関連項目

额外PARP抗体，包括PARP-1, PARP-10, PARP-14, PARP-2, PARP-3和PARP-4

pADPr抗体（10H）是通过WB、IF和ELISA检测pADPr蛋白的IgG3小鼠单克隆pADPrs抗体（也称为多（ADP-核糖）抗体、标准杆数抗体或ADP-核糖聚合物抗体）。pADPr抗体（10H）可作为非缀合的抗pADPro抗体获得。聚ADP核糖基化是一种DNA损伤依赖性翻译后修饰的方法，有助于挽救受伤的增殖细胞免于细胞死亡。PARP（聚[ADP核糖]聚合酶）蛋白包括一个功能性修饰组蛋白和其他核蛋白的酶超家族，从而防止细胞死亡。PARP使用NAD+作为底物将ADP核糖残基催化转移到蛋白质受体上；当重复多次时，这一过程导致形成独立存在或连接到靶蛋白的聚ADP核糖（pADPr）链。这些链的存在改变了靶蛋白的功能并促进细胞存活。此外，pADPr链被认为对细胞周期进展和细胞对DNA损伤的反应至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

pADPr 抗体 (10H) 参考文献:

  1. 过表达显性阴性 PARP 会干扰 HeLa 细胞在裸鼠体内形成肿瘤：这是体内肿瘤细胞凋亡增加的证据。  |  Hans, MA., et al. 1999. Oncogene. 18: 7010-5. PMID: 10597301
  2. 分析完整细胞中 ADP 核糖聚合物的大小。  |  Gagné, JP., et al. 2001. Mol Cell Biochem. 224: 183-5. PMID: 11693195
  3. B 型侵袭性淋巴瘤家族蛋白具有独特的结构域，可调节转录并具有聚（ADP-核糖）聚合酶活性。  |  Aguiar, RC., et al. 2005. J Biol Chem. 280: 33756-65. PMID: 16061477
  4. CDK 依赖性激活聚（ADP-核糖）聚合酶成员 10 (PARP10)。  |  Chou, HY., et al. 2006. J Biol Chem. 281: 15201-7. PMID: 16455663
  5. 聚（ADP-核糖）新陈代谢的作用不断扩大：当前的挑战和新的前景。  |  Gagné, JP., et al. 2006. Curr Opin Cell Biol. 18: 145-51. PMID: 16516457
  6. Stat6 协作者（CoaSt6）缔合作用的聚（ADP-核糖）聚合酶活性可调节 Stat6 依赖性基因转录。  |  Goenka, S., et al. 2007. J Biol Chem. 282: 18732-9. PMID: 17478423
  7. 哺乳动物 PARPS--一个小而强大的聚-ADP-核糖聚合酶家族--的多种生物学作用。  |  Hassa, PO. and Hottiger, MO. 2008. Front Biosci. 13: 3046-82. PMID: 17981777
  8. 聚（ADP-核糖）聚合酶 1 通过协同激活缺氧诱导因子-1 依赖性基因的表达，促进肿瘤细胞的存活。  |  Elser, M., et al. 2008. Mol Cancer Res. 6: 282-90. PMID: 18314489
  9. 聚（ADP-核糖）聚合酶的活性调节烷基化 DNA 损伤后 HeLa 细胞的凋亡。  |  Liu, X., et al. 2008. Cancer Biol Ther. 7: 934-41. PMID: 18376143
  10. 非共价pADPr与蛋白质的相互作用以及与RNA竞争与蛋白质的结合。  |  Ji, Y. 2011. Methods Mol Biol. 780: 83-91. PMID: 21870256
  11. 果蝇组织中pADPr的免疫检测：厚切片的免疫染色；免疫电镜。  |  Jarnik, M. 2011. Methods Mol Biol. 780: 401-11. PMID: 21870274
  12. 体内检测和定量pADPr。  |  Lamade, AM., et al. 2023. Methods Mol Biol. 2609: 23-42. PMID: 36515827

订购信息

产品名称产品编号规格价格数量收藏夹

pADPr 抗体 (10H)

sc-56198
100 µg/ml
RMB2377.00

pADPr (10H): m-IgG3 BP-HRP 套装

sc-550377
100 µg Ab; 40 µg BP
RMB2662.00

Is there a carrier-free version of sc-56198? Perhaps custom? Need to label it with a fluorophore for flow cytometry.

Asked by: pkiela
Thank you for your question. For carrier free products please contact special orders by emailing [email protected]
Answered by: BlakeJ
Date published: 2023-05-31

Is this antibody suitable for immunoprecipitation studies?

Asked by: Irshad
Thank you for your question. We haven’t tested this antibody for immunoprecipitation (IP) and so that is not a recommended application at this time. However, we are aware of a few product citations which cite this antibody for that application. Please use the product citation search on our website to search for those specific publications. Or please give us a call in the Technical Service Department for support. https://www.scbt.com/scbt/misc/citationSearch.jsp
Answered by: Technical Support
Date published: 2025-04-30

How much sample should be loaded on SDS-PAGE for detecting pADPr using this antibody?

Asked by: MLis344
Thank you for your question. We recommend loading 40-60µg of sample on your WB for this antibody. Please contact Technical Service if you have any further questions.
Answered by: Technical Support
Date published: 2019-01-26

What is the most recommended diluent for this primary antibody? Is it ok to use 5% BSA in the solution? Thank you in advance.

Asked by: Lucius
Thank you for your question. We recommend diluting the primary antibodies in 5% milk with TBS-T. It is OK to use BSA, but we test them using milk as it tends to give cleaner results. Please contact technical support via phone, email or chat if you have any further questions!
Answered by: Tech Service
Date published: 2020-11-24

I am all out of my donkey anti-mouse secondary antibody, what should I use instead?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-29
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Rated 5 out of 5 by from Works excellentlyWe used 10H to detect the photocrosslinking activity between 10H antibody and poly-ADP-ribosyl(PAR). Such detection requires antibody to be equipped with superb purity and stability. 10H from Santa Cruz Biotech always give us reproducible results with great sensitivity. The ultra performance of 10H has encouraged us to keep using it in our upcoming Photocrossliking-series research!
Date published: 2021-04-23
Rated 4 out of 5 by from It works for western blotting!It detected high molecular size bands, as expected for poly-ADP-ribosylated proteins, in total nuclear protein lysates from HEK 293T cells (1:500 dilution in 5% milk in TBS/0.1% Tween-20 overnight at 4C). Though, I recommend checking antibody specificity with PARP1-specific siRNAs or pharmacological inhibitors.
Date published: 2018-07-30
Rated 5 out of 5 by from 对实验很有帮助！这个产品对我们的实验帮助很大，这个抗体只有SC才有！使用这个产品之后，做出了很理想的结果！对以后发表文章有用。
Date published: 2018-03-12
Rated 3 out of 5 by from pADPr 10H AntibodyTested for Western Blot analysis in cells collected 15 and 30’ minutes after H2O2 addition and a pre-treatment of 30 minutes of Veliparib, with the relative controls. The signal is not very strong but good and well evaluable.
Date published: 2018-01-15
Rated 4 out of 5 by from Specific detection of poly-ADP-riboseI used this antibody in western blot to detect PAR polymer in cells treated with hydrogen peroxide and LPS. The most striking difference between treated and untreated cells was achieved when membranes were blocked with 0.5% gelatin.
Date published: 2017-12-01
Rated 1 out of 5 by from No IF signalAfter getting a sample, I tried this antibody several times with no success. I used it on both Histochoice and 4% PFA fixed fibroblasts, and used it at dilutions of 1:200, 1:100, and 1:50, and even tried a 1hr DAKO antigen retrieval incubation at 95C, after speaking to technical support, all without being able to get any specific signal.
Date published: 2017-11-03
Rated 4 out of 5 by from well-established Ab for PAR detectionthe antibody works well for IF (1:100) and IP (1-1.5ug); however, it does seem somewhat less robust than preparations I have ordered from other suppliers (at the same concentration)
Date published: 2017-06-01
Rated 5 out of 5 by from Worked wellWorked well in IF in human Hela cells, very satisfied with the performance of the antibody.
Date published: 2017-05-28
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pADPr 抗体 (10H) is rated 4.1 out of 5 by 16.
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