Date published: 2025-9-8
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p68 RNA Helicase Antibody (D-7) - Western Blotting - Image 359792
p68 RNA 螺旋酶抗体 (D-7): sc-365164. Hep G2 (A), SH-SY5Y (B), NIH/3T3 (C) 和 HeLa (D) 全细胞裂解液中 p68 RNA Helicase 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
p68 RNA 螺旋酶抗体 (D-7): sc-365164. Hep G2 (A), SH-SY5Y (B), NIH/3T3 (C) 和 HeLa (D) 全细胞裂解液中 p68 RNA Helicase 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

p68 RNA Helicase 抗体 (D-7): sc-365164

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说明书
  • p68 RNA Helicase 抗体 D-7 是小鼠单克隆 IgG2a κ，p68 RNA Helicase抗体, 在10篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的p68 RNA Helicase的C-terminus附近的氨基酸547-579之间
  • p68 RNA Helicase 抗体 (D-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 p68 RNA Helicase; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 抗p68 RNA Helicase抗体（D-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p68 RNA Helicase (D-7): sc-365164的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是p68 RNA Helicase Antibody (D-7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与p68 RNA Helicase Antibody (D-7) 打包提供（请参阅下面的订购信息）。

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描述
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说明书与实验方案
研究信息

関連項目

p68 RNA解旋酶抗体（D-7）是一种IgG2a κ小鼠单克隆p68 RNA解旋酶抗体（也称为DEAD-Box解旋酶5抗体、DEAD/H (Asp-Glu-Ala-Asp/His) Box多肽5（RNA解旋酶68kD）抗体、DEAD (Asp-Glu-Ala-Asp) Box解旋酶5抗体、DEAD Box蛋白5抗体、RNA解旋酶P68抗体、G17P1抗体、HLR1抗体、ATP依赖性RNA解旋酶DDX5抗体、DEAD Box-5抗体或HUMP68抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的p68 RNA解旋酶蛋白。p68 RNA解旋酶抗体（D-7）既可作为非偶联抗p68 RNA解旋酶抗体形式提供，也可作为多种偶联形式的抗p68 RNA解旋酶抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。p68 RNA解旋酶是一种具有RNA依赖性ATP酶活性的核蛋白。蛋白激酶C的磷酸化抑制了p68 RNA解旋酶的活性。p68 RNA解旋酶似乎在发育过程中的器官分化中发挥作用。此外，p68 RNA解旋酶在多种不同脊索动物胚胎的早期神经发育和各种中胚层组织中表达。在细胞水平上，p68 RNA解旋酶的表达水平在血清诱导的静止细胞系中增加。p68 RNA解旋酶可能作为雌激素受体α的共激活剂。此外，p68 RNA解旋酶与转录共激活剂CBP和p300相关联。在正常情况下，p68 RNA解旋酶定位于细胞核。在晚期末期，p68 RNA解旋酶和fibrillarin共同定位于新生的核仁。p68 RNA解旋酶可能与p72 RNA解旋酶形成异二聚体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p68 RNA Helicase 抗体 (D-7) 参考文献:

  1. 纯化并鉴定作为人类雌激素受体 alpha 激活功能 1 特异性转录辅激活子的 p68 RNA 螺旋酶。  |  Endoh, H., et al. 1999. Mol Cell Biol. 19: 5363-72. PMID: 10409727
  2. 核DEAD盒RNA螺旋酶p68与核小体蛋白纤维蛋白相互作用，并在端期特异性地聚集在新生核小体中。  |  Nicol, SM., et al. 2000. Exp Cell Res. 257: 272-80. PMID: 10837141
  3. p68是一种DEAD-box RNA螺旋酶，在脊索动物胚胎神经和中胚层组织中表达。  |  Seufert, DW., et al. 2000. J Exp Zool. 288: 193-204. PMID: 11069138
  4. p68 RNA 螺旋酶与转录辅激活因子 CBP 和 p300 之间的协同作用。  |  Rossow, KL. and Janknecht, R. 2003. Oncogene. 22: 151-6. PMID: 12527917
  5. 细胞中高度相关的 DEAD box RNA 螺旋酶 p68 和 p72 以异二聚体形式存在。  |  Ogilvie, VC., et al. 2003. Nucleic Acids Res. 31: 1470-80. PMID: 12595555
  6. P68 RNA螺旋酶通过使Axin脱离β-catenin，介导PDGF诱导的上皮间充质转化。  |  Yang, L., et al. 2006. Cell. 127: 139-55. PMID: 17018282
  7. P68 RNA 螺旋酶能解链人类 let-7 microRNA 前体双链，是 let-7 引导的基因表达沉默所必需的。  |  Salzman, DW., et al. 2007. J Biol Chem. 282: 32773-9. PMID: 17724023
  8. p68 RNA 螺旋酶通过直接调控 NF-κB 转录因子 p50 促进胶质瘤细胞在体外和体内增殖。  |  Wang, R., et al. 2012. Neuro Oncol. 14: 1116-24. PMID: 22810421
  9. 与人类 p68 蛋白相关的 RNA 螺旋酶活性。  |  Hirling, H., et al. 1989. Nature. 339: 562-4. PMID: 2471939
  10. 通过分子动力学模拟分析 β-catenin 和 RX-5902 与磷酸化 p68 RNA 螺旋酶结合的结构特征。  |  Ali, W., et al. 2018. Prog Biophys Mol Biol. 140: 79-89. PMID: 29729328
  11. 钙调蛋白和蛋白激酶 C 对 p68 RNA 螺旋酶的调控  |  Buelt, MK., et al. 1994. J Biol Chem. 269: 29367-70. PMID: 7525583
  12. 死盒 'RNA 螺旋酶 p68 的表达受发育和生长调控，并与胎儿器官分化/成熟相关。  |  Stevenson, RJ., et al. 1998. J Pathol. 184: 351-9. PMID: 9664900

订购信息

产品名称产品编号规格价格数量收藏夹

p68 RNA Helicase 抗体 (D-7)

sc-365164
200 µg/ml
RMB2377.00

p68 RNA Helicase (D-7): m-IgG Fc BP-HRP 套装

sc-529318
200 µg Ab; 10 µg BP
RMB2662.00

p68 RNA Helicase (D-7): m-IgGκ BP-HRP 套装

sc-522150
200 µg Ab, 40 µg BP
RMB2662.00

p68 RNA Helicase (D-7): m-IgG2a BP-HRP 套装

sc-547297
200 µg Ab; 10 µg BP
RMB2662.00

p68 RNA Helicase 抗体 (D-7) AC

sc-365164 AC
500 µg/ml, 25% agarose
RMB3129.00

p68 RNA Helicase 抗体 (D-7) HRP

sc-365164 HRP
200 µg/ml
RMB2377.00

p68 RNA Helicase 抗体 (D-7) FITC

sc-365164 FITC
200 µg/ml
RMB2482.00

p68 RNA Helicase 抗体 (D-7) PE

sc-365164 PE
200 µg/ml
RMB2580.00

p68 RNA Helicase 抗体 (D-7) Alexa Fluor® 488

sc-365164 AF488
200 µg/ml
RMB2685.00

p68 RNA Helicase 抗体 (D-7) Alexa Fluor® 546

sc-365164 AF546
200 µg/ml
RMB2685.00

p68 RNA Helicase 抗体 (D-7) Alexa Fluor® 594

sc-365164 AF594
200 µg/ml
RMB2685.00

p68 RNA Helicase 抗体 (D-7) Alexa Fluor® 647

sc-365164 AF647
200 µg/ml
RMB2685.00

p68 RNA Helicase 抗体 (D-7) Alexa Fluor® 680

sc-365164 AF680
200 µg/ml
RMB2685.00

p68 RNA Helicase 抗体 (D-7) Alexa Fluor® 790

sc-365164 AF790
200 µg/ml
RMB2685.00

p68 RNA Helicase (D-7) 中和勝肽

sc-365164 P
100 µg/0.5 ml
RMB511.00

Hi All, Do you know, who had established the hybridoma that generates this antibody ?

Asked by: Peter28
Thank you for your question. This antibody was created against amino acid range 547-579 of human p68 RNA Helicase in our own labs.
Answered by: Tech Support Europe
Date published: 2021-03-22

What application is the blocking peptide sc-365164 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Very goodVery nice signal in WB of total lysates of human cell lines.
Date published: 2020-05-26
Rated 5 out of 5 by from very goodthis ddx5 antibody is very well, i usually use 1 ul antibody in 1000ul 5% bsa for WB.
Date published: 2017-12-01
Rated 5 out of 5 by from Nice antibodyPerfect results in a 1:5000 dilution over night. Clean bands without any background.
Date published: 2017-11-28
Rated 4 out of 5 by from very good, clean antibodyI used it for western blotting and for immunoprecipitation from cell lines of human origin. It worked very well in both applications.
Date published: 2017-10-04
Rated 5 out of 5 by from Great for WB and IPUsed at 1:1000 for detection of DDX5 in nuclear extracts of HEK293T cells and yielded specific and decent band. Also efficiently immunoprecipitates endogenous protein at 2ug/ml nuclear extract.
Date published: 2017-09-20
Rated 4 out of 5 by from Good Ab for WBIt is good for western-blot in breast cancer cells
Date published: 2017-08-10
Rated 5 out of 5 by from Very good at Western BlottinWe tested p68 antibody in Western Blotting and it worked pretty good at a dilution of 1:1000 over night
Date published: 2017-08-03
Rated 5 out of 5 by from good IFGood IF in formalin-fixed HepG2 cells, strong staining of HepG2 cells.
Date published: 2017-05-07
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p68 RNA Helicase 抗体 (D-7) is rated 4.8 out of 5 by 12.
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