p35 抗体 (4G11): sc-293184

質問頂き、誠にありがとうございます。CDK5R1はp35の別名である、この遺伝子によってコードされるタンパク質（p35）は、サイクリン依存性キナーゼ5（CDK5）のニューロン特異的活性化因子であり、CDK5の活性化は中枢神経系の適切な発達に必要である。https://www.uniprot.org/uniprotkb/Q15078/entry#names_and_taxonomy CDK5R1 is another name for p35.The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. https://www.uniprot.org/uniprotkb/Q15078/entry#names_and_taxonomy

Thank you for your question. According to Uniprot, (http://www.uniprot.org/uniprot/Q15078#ptm_processing), the cleaved p25 form of p35 corresponds to amino acids 99-307. This mouse monoclonal antibody, p35 (4G11): sc-293184, is raised against amino acids 208-307 of human p35. Therefore, the antibody does have an epitope that should recognize the cleaved p25 form. We have not confirmed this experimentally.

Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining