Date published: 2025-9-8

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p300 抗体 (NM11): sc-32244

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说明书
  • p300抗体(NM11)是小鼠单克隆IgG2b κ, 在50篇文献中引用,规格为200 µg/ml
  • 亲和纯化的 p300 of human 起源
  • 抗-p300 抗体 (NM11) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 p300 and CBP (CREB-binding protein)
  • 抗p300抗体(NM11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-32244 X, 200 µg/0.1 ml)
  • 关于如何获取p300 (NM11): sc-32244免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 p300 抗体 (NM11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与p300 抗体 (NM11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p300抗体(NM11)是一种小鼠单克隆IgG2b kappa轻链抗体,用于在小鼠、大鼠和人类样本中检测p300蛋白(也称为EP300)及其密切相关的对应物CBP(CREB结合蛋白或CREBBP),检测方法包括蛋白质印迹(WB )、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)检测小鼠、大鼠和人类样本中的p300蛋白(又称EP300)及其密切相关的蛋白CBP(CREB结合蛋白或CREBBP)。p300蛋白是一种重要的转录共激活因子,通过改变染色质结构和促进转录复合物的组装来调节基因表达,这对于细胞生长、分化、凋亡等过程至关重要。p300主要位于细胞核中,通过与各种转录因子和核受体相互作用,在基因调控中发挥关键作用,从而影响多种细胞通路并保持细胞稳态。从结构上看,p300具有多个结构域,包括溴结构域和HAT(组蛋白乙酰转移酶)结构域,这些结构域对于组蛋白和其他蛋白质的乙酰化至关重要,从而调节基因可及性和转录活性。p300单克隆抗体(NM11) 有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®变体,为不同的实验应用提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p300 抗体 (NM11)

sc-32244
200 µg/ml
RMB2377.00

p300 (NM11): m-IgG Fc BP-HRP 套装

sc-525532
200 µg Ab; 10 µg BP
RMB2662.00

p300 抗体 (NM11) X

sc-32244 X
200 µg/0.1 ml
RMB2377.00

p300 抗体 (NM11) AC

sc-32244 AC
500 µg/ml, 25% agarose
RMB3129.00

p300 抗体 (NM11) HRP

sc-32244 HRP
200 µg/ml
RMB2377.00

p300 抗体 (NM11) FITC

sc-32244 FITC
200 µg/ml
RMB2482.00

p300 抗体 (NM11) PE

sc-32244 PE
200 µg/ml
RMB2580.00

p300 抗体 (NM11) Alexa Fluor® 488

sc-32244 AF488
200 µg/ml
RMB2685.00

p300 抗体 (NM11) Alexa Fluor® 546

sc-32244 AF546
200 µg/ml
RMB2685.00

p300 抗体 (NM11) Alexa Fluor® 594

sc-32244 AF594
200 µg/ml
RMB2685.00

p300 抗体 (NM11) Alexa Fluor® 647

sc-32244 AF647
200 µg/ml
RMB2685.00

p300 抗体 (NM11) Alexa Fluor® 680

sc-32244 AF680
200 µg/ml
RMB2685.00

p300 抗体 (NM11) Alexa Fluor® 790

sc-32244 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-32244 X be used instead of the standard form sc-32244?

Asked by: jenniferc
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 15
Date published: 2017-01-19
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Rated 4 out of 5 by from It works fine for western blottingI used this ab sample a few days ago. It works fine for western blotting
Date published: 2021-11-10
Rated 4 out of 5 by from Good for WBI bought this ab a few weeks ago and did WB with whole cell lysate. The result is good.
Date published: 2021-06-03
Rated 4 out of 5 by from Good ABThis AB works in IF (1/30) for fibroblast cells (PFA fixation)
Date published: 2018-03-22
Rated 1 out of 5 by from No WB band for mouse samplesI did 1:500 dilution but didn't see any band at the expected size.
Date published: 2018-01-11
Rated 2 out of 5 by from Faint band in western blotting!It detected a very faint band in the size range expected for endogenous p300 in total protein extracts of immortalized HFFs (1:500 in 5% milk/TBS-Tween, overnight at 4C).
Date published: 2017-11-27
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Superb product !!Strong clear western blot data; also very nice IHC staining data
Date published: 2017-02-01
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of p300 expression in Jurkat nuclear extract.
Date published: 2017-01-11
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p300 抗体 (NM11) is rated 3.9 out of 5 by 14.
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