Date published: 2025-9-8
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p300 Antibody (F-4) - Immunohistochemistry - Image 156757
p300 抗体 (F-4): sc-48343. 对福尔马林固定, 石蜡包埋的人体直肠组织进行免疫过氧化物酶染色, 显示腺细胞亚群的细胞核和细胞质染色.
p300 抗体 (F-4): sc-48343. 对福尔马林固定, 石蜡包埋的人体直肠组织进行免疫过氧化物酶染色, 显示腺细胞亚群的细胞核和细胞质染色.

p300 抗体 (F-4): sc-48343

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说明书
  • p300 抗体 F-4 是小鼠单克隆 IgG2a κ，p300抗体, 在104篇文献中引用，规格为200 µg/ml
  • 免疫human物种的p300的氨基酸774-1045
  • p300 抗体 (F-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 p300
  • 抗p300抗体（F-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-48343 X, 200 µg/0.1 ml)
  • 关于如何获取p300 (F-4): sc-48343的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 p300 抗体 (F-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p300 抗体 (F-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p300抗体（F-4）是一种小鼠单克隆IgG2a kappa轻链抗体，也称为KAT3B或EP300抗体，用于通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组化 与石蜡包埋切片（IHCP）和酶联免疫吸附测定（ELISA）一起使用。p300是一种重要的转录共激活因子和组蛋白乙酰转移酶（HAT），通过改变染色质结构在调节基因表达方面发挥着重要作用，而染色质结构对于细胞生长、分化及凋亡等过程至关重要。p300主要位于细胞核中，与多种转录因子相互作用，包括CREB（cAMP反应元件结合蛋白）和CBP（CREB结合蛋白），促进转录复合物的组装，从而维持细胞内环境稳定。p300的翻译后修饰（如乙酰化和磷酸化）对于调节功能以及与其他蛋白相互作用的能力至关重要，从而动态调节基因表达。在结构上，p300具有多个结构域，包括溴结构域和HAT结构域，这些结构域对于酶促活性和与乙酰化组蛋白及其他蛋白的相互作用至关重要。p300单克隆抗体（F-4）针对人类p300的氨基酸774-1045。抗 300抗体（F-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®变体，为各种实验应用提供了多功能性。通过靶向p300，研究人员可以研究癌症、发育生物学和其他疾病的关键通路，在这些疾病中，p300介导的转录调控至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p300 抗体 (F-4) 参考文献:

  1. 转录共激活因子和乙酰转移酶p300在成纤维细胞生物学和纤维化中的作用。  |  Ghosh, AK. and Varga, J. 2007. J Cell Physiol. 213: 663-71. PMID: 17559085
  2. 转录共激活因子p300的生死。  |  Chen, J. and Li, Q. 2011. Epigenetics. 6: 957-61. PMID: 21730760
  3. 转录共激活因子p300和CBP的基因组占用。  |  Holmqvist, PH. and Mannervik, M. 2013. Transcription. 4: 18-23. PMID: 23131664
  4. P300抑制增强吉西他滨诱导的胰腺癌细胞凋亡。  |  Ono, H., et al. 2016. Oncotarget. 7: 51301-51310. PMID: 27322077
  5. 乙酰转移酶p300调节NRF2的稳定性和定位。  |  Ganner, A., et al. 2020. Biochem Biophys Res Commun. 524: 895-902. PMID: 32057361
  6. 组蛋白乙酰转移酶CBP和p300在转录和功能水平上调节滑膜成纤维细胞应激反应通路。  |  Krošel, M., et al. 2023. Sci Rep. 13: 17112. PMID: 37816914
  7. 腺病毒 E1A 相关 300-kD 蛋白（p300）的分子克隆和功能分析揭示了一种具有转录适配器特性的蛋白质。  |  Eckner, R., et al. 1994. Genes Dev. 8: 869-84. PMID: 7523245
  8. E1A 肿瘤蛋白靶向的转录适配蛋白家族。  |  Arany, Z., et al. 1995. Nature. 374: 81-4. PMID: 7870178
  9. 腺病毒 E1A 相关蛋白 p300 作为转录共激活因子 CBP 的功能同源物。  |  Lundblad, JR., et al. 1995. Nature. 374: 85-8. PMID: 7870179
  10. E1A 相关 p300 和 CREB 相关 CBP 属于一个保守的辅助激活子家族。  |  Arany, Z., et al. 1994. Cell. 77: 799-800. PMID: 8004670
  11. 磷酸化的 CREB 能与核蛋白 CBP 特异性结合。  |  Chrivia, JC., et al. 1993. Nature. 365: 855-9. PMID: 8413673
  12. 核蛋白 CBP 是转录因子 CREB 的辅助激活因子。  |  Kwok, RP., et al. 1994. Nature. 370: 223-6. PMID: 7913207

订购信息

产品名称产品编号规格价格数量收藏夹

p300 抗体 (F-4)

sc-48343
200 µg/ml
RMB2377.00

p300 (F-4): m-IgG Fc BP-HRP 套装

sc-528519
200 µg Ab; 10 µg BP
RMB2662.00

p300 (F-4): m-IgGκ BP-HRP 套装

sc-520915
200 µg Ab, 40 µg BP
RMB2662.00

p300 抗体 (F-4) X

sc-48343 X
200 µg/0.1 ml
RMB2377.00

p300 抗体 (F-4) AC

sc-48343 AC
500 µg/ml, 25% agarose
RMB3129.00

p300 抗体 (F-4) HRP

sc-48343 HRP
200 µg/ml
RMB2377.00

p300 抗体 (F-4) FITC

sc-48343 FITC
200 µg/ml
RMB2482.00

p300 抗体 (F-4) PE

sc-48343 PE
200 µg/ml
RMB2580.00

p300 抗体 (F-4) Alexa Fluor® 488

sc-48343 AF488
200 µg/ml
RMB2685.00

p300 抗体 (F-4) Alexa Fluor® 546

sc-48343 AF546
200 µg/ml
RMB2685.00

p300 抗体 (F-4) Alexa Fluor® 594

sc-48343 AF594
200 µg/ml
RMB2685.00

p300 抗体 (F-4) Alexa Fluor® 647

sc-48343 AF647
200 µg/ml
RMB2685.00

p300 抗体 (F-4) Alexa Fluor® 680

sc-48343 AF680
200 µg/ml
RMB2685.00

p300 抗体 (F-4) Alexa Fluor® 790

sc-48343 AF790
200 µg/ml
RMB2685.00

I would like to test this antibody for RIP-seq in hamster cells. Could I get an aliquote to validate it via WB? Thanks a lot

Asked by: CateR
Thank you for your question. Please contact our technical service team directly to assist with your sample request.
Answered by: Tech Support Europe
Date published: 2022-02-01

I would like to get a FREE 10 µg sample of p300 (F-4): sc-48343. Thanks a lot !

Asked by: Lily18
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-15

I would like to receive a FREE 10 µg sample of p300 (F-4): sc-48343. Best regards

Asked by: Tomasz
Thank you for the question and your interest in our products. We will contact you by email today.
Answered by: Technical Support Europe
Date published: 2018-08-01

I have failed several times with NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels and transfer with iBlot2 for 10 minute. Do you think this gel is qualified to detect a protein located in 300 kDa? And do you have any suggestion? should I load more sample?

Asked by: Jack1109
Thank you for your question. We have not specifically used gels and transfer system that you are using. You may want to contact those suppliers for more information about the suitability of their reagents. In our experience with our p300 Antibody (F-4): sc-48343, we recommend using a low-concentration gel and longer transfer times to a PVDF membrane (rather than nitrocellulose) for detection of a larger protein such as p300. We recommend loading 40-60 µg of nuclear extract protein for p300 (e.g., HeLa, MCF7, Jurkat or A-431). For large proteins, transfer out of the gel may be very slow, just as the larger proteins run slowly within the gel during separation. If blotting a large protein, we recommend to run your samples in a low-concentration gel, 8% or less. These gels will be very fragile, so handle carefully. We also recommend to choose wet transfer overnight at 4C instead of semi-dry transfer. The following reference discusses a gel and buffer system that allows transfer of proteins as large as 500 kD: Bolt and Mahoney, High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate– polyacrylamide, gel electrophoresis. Analytical Biochemistry 247, 185–192 (1997).
Answered by: Technical Support
Date published: 2017-02-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-48343 PE), fluorescein (sc-48343 FITC), Alexa Fluor® 488 (sc-48343 AF488) or Alexa Fluor® 647 (sc-48343 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-10
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Rated 2 out of 5 by from weak signal, high backgroundnot very happy with this antibody. will not buy full size vial.
Date published: 2025-07-22
Rated 1 out of 5 by from p300 ChIPUpon receipt of the Ab, we have kept it at 4C (~1yr). We performed a p300 ChIP-Seq in mouse cell lines and pulled nothing out. Looks like the antibody does not work.
Date published: 2023-11-20
Rated 5 out of 5 by from Excelente Resultado!Anteriormente, utilizábamos los anticuerpos anti-p300 (C-20): sc-585 y (N-15): sc-584 pero se nos acabaron. Ambos funcionaban muy bien para técnicas como WB, IHQ e IF, obtuvimos excelentes resultados, pero en la página ya no figuran más y fueron reemplazados por: p300 (F-4): sc-48343. Nuestro laboratorio habitualmente compra anticuerpos de Santa Cruz. Adjunto publicación: -Fermento et al, 2014. Inhibition of p300 suppresses growth of breast cancer. Role of p300. subcellular localization.
Date published: 2022-09-16
Rated 5 out of 5 by from It worked!Used in WB with Licor Odyssey system with recommended dilution and saw a signal
Date published: 2022-08-14
Rated 4 out of 5 by from Great antibodyIt detectes a specific band in WB. Works in IF and PLA assays. works in ChIP-qPCR. But using 2ug of this antibody in co-IP we couldn't Immunoprecipitate Skip.
Date published: 2020-02-17
Rated 1 out of 5 by from No signalThe antibody doesn't detect any protein at 1:1000 and 1:250 dilutions. Poor
Date published: 2018-10-02
Rated 2 out of 5 by from AB not great for IFI did not get a good signal with this AB on IF (1/50 , PFA fixation) should be OK for WB
Date published: 2018-03-22
Rated 2 out of 5 by from p300We detected p300 (300 kDa) in mouse cell line. However we also detected another band (around 200 kDa) that may be degraded form.
Date published: 2018-01-11
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p300 抗体 (F-4) is rated 4.1 out of 5 by 40.
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