Date published: 2025-9-8
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p14ARF Antibody (DCS-240) - Western Blotting - Image 397970
p14 ARF 抗体 (DCS-240): sc-53639. DU 145 全细胞裂解液 (A) 和 HeLa 核提取物 (B) 中 p14 ARF 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
p14ARF Antibody (DCS-240) - Western Blotting - Image 397970
p14 ARF 抗体 (DCS-240): sc-53639. DU 145 全细胞裂解液 (A) 和 HeLa 核提取物 (B) 中 p14 ARF 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
p14ARF Antibody (DCS-240) - Western Blotting - Image 14586
p14 ARF 抗体 (DCS-240): sc-53639. Western 印迹分析 BJAB 全细胞裂解液中 p14 ARF 的表达.
p14 ARF 抗体 (DCS-240): sc-53639. DU 145 全细胞裂解液 (A) 和 HeLa 核提取物 (B) 中 p14 ARF 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

p14ARF 抗体 (DCS-240): sc-53639

4.9(8)
寫評論提問

说明书
  • p14ARF抗体(DCS-240)是小鼠单克隆IgG1 κ, 在14篇文献中引用，规格为200 µg/ml
  • 针对p14 ARF的human起源的N-terminus的
  • p14ARF 抗体 (DCS-240) 推荐用于WB, IP 和 IF，检测human 来源的 p14 ARF
  • 抗p14ARF抗体（DCS-240）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p14ARF (DCS-240): sc-53639的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是p14ARF Antibody (DCS-240) for WB applications. 的首选辅助检测试剂，这些试剂现与p14ARF Antibody (DCS-240) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加p16 INK4A/p19 ARF/p14 ARF (CDKN2A)抗体，包括p14 ARF和p19 ARF

p14 ARF 抗体（DCS-240）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测人源 p14 ARF。p14 ARF 单克隆抗体（DCS-240）有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 共轭物。p14 ARF（DCS-240）抗体可识别一种在调节细胞周期中起关键作用的蛋白质，它是一种肿瘤抑制因子，对维持细胞完整性和防止细胞失控增殖至关重要。通过与 MDM2 结合，p14 ARF 稳定了 p53 的水平，导致 p21 的表达增加，细胞周期随之停滞在 G1 和 G2/M 阶段。p14 ARF 调节这些关键通路的能力突出了抗 p14 ARF 抗体（DCS-240）在细胞周期调控和肿瘤抑制研究中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p14ARF 抗体 (DCS-240)

sc-53639
200 µg/ml
RMB2377.00

p14ARF (DCS-240): m-IgG Fc BP-HRP 套装

sc-526839
200 µg Ab; 10 µg BP
RMB2662.00

p14ARF (DCS-240): m-IgG1 BP-HRP 套装

sc-532212
200 µg Ab; 20 µg BP
RMB2662.00

p14ARF 抗体 (DCS-240) AC

sc-53639 AC
500 µg/ml, 25% agarose
RMB3129.00

p14ARF 抗体 (DCS-240) HRP

sc-53639 HRP
200 µg/ml
RMB2377.00

p14ARF 抗体 (DCS-240) FITC

sc-53639 FITC
200 µg/ml
RMB2482.00

p14ARF 抗体 (DCS-240) PE

sc-53639 PE
200 µg/ml
RMB2580.00

p14ARF 抗体 (DCS-240) Alexa Fluor® 488

sc-53639 AF488
200 µg/ml
RMB2685.00

p14ARF 抗体 (DCS-240) Alexa Fluor® 546

sc-53639 AF546
200 µg/ml
RMB2685.00

p14ARF 抗体 (DCS-240) Alexa Fluor® 594

sc-53639 AF594
200 µg/ml
RMB2685.00

p14ARF 抗体 (DCS-240) Alexa Fluor® 647

sc-53639 AF647
200 µg/ml
RMB2685.00

p14ARF 抗体 (DCS-240) Alexa Fluor® 680

sc-53639 AF680
200 µg/ml
RMB2685.00

p14ARF 抗体 (DCS-240) Alexa Fluor® 790

sc-53639 AF790
200 µg/ml
RMB2685.00

I would like to know if p14ARF antibody (DCS-240) is better diluted in milk or BSA 5%. Thank you!

Asked by: Gpb27
Thank you for your question. Ideal blocking conditions are dependent on a variety of different factors. If your western blots show a lot of background, milk will provide a broader blocking effect, while a BSA-based blocking buffer will prevent overblocking of weaker signals.
Answered by: Tech Support Europe
Date published: 2020-12-01

Is this antibody specific for p14 instead of p16 or can it detect both p14 and p16 from human cells? Thank you!

Asked by: Mai Xu
Thank you for your question. This monoclonal antibody is specific for p14 ARF of human origin.
Answered by: Technical Support
Date published: 2019-06-16

I would like to use p14 ARF Antibody (DCS-240): sc-53639 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-53639 is not available directly conjugated, but we have an alternative p14 ARF antibody (sc-53392) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-02-08
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Rated 4 out of 5 by from Work for In cell westernI only tried this antibody for in cell western and it works fine in dilution 1:400.
Date published: 2018-01-30
Rated 5 out of 5 by from Very popular choice!This monoclonal p14 ARF Antibody (DCS-240) is a very popular choice, especially for Western blotting of human samples, as indicated by the many product citations for this clone.
Date published: 2017-01-26
Rated 5 out of 5 by from OKAntibody was published for use in IP and WB on human Hela and H1299 Cells. the wb:1:500 is OK
Date published: 2016-12-07
Rated 5 out of 5 by from Antibody was published for use in IP andAntibody was published for use in IP and WB on human 293T and H1299 cell - SCBT Publication Review
Date published: 2015-04-27
Rated 5 out of 5 by from Nice Western blot data obtained in BJAB wholeNice Western blot data obtained in BJAB whole cell lysate. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from WB publishable data with human stable fibroblastWB publishable data with human stable fibroblast cell lines - SCBT Publication Review
Date published: 2015-01-27
Rated 5 out of 5 by from Good data published with this antibody inGood data published with this antibody in WB on immortalized human fibroblast cell line (hTeRT-BJ1 cells) - SCBT Publication Review
Date published: 2015-01-05
Rated 5 out of 5 by from Publishable WB in human MDAPublishable WB in human MDA-MB-231 cells and hTERT-BJ1 cells -SCBT Publication Review
Date published: 2013-10-03
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p14ARF 抗体 (DCS-240) is rated 4.9 out of 5 by 8.
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