Date published: 2025-9-8
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CDKN2A/p16 Antibody (JC8) - Western Blotting - Image 16201
p16 INK4A 抗体 (JC8): sc-56330. HeLa (A), Saos-2 (B), SHP-77 (C) 和 ME-180 (D) 全细胞裂解液中 p16 INK4A 表达的 Western 印迹分析.
p16 INK4A 抗体 (JC8): sc-56330. HeLa (A), Saos-2 (B), SHP-77 (C) 和 ME-180 (D) 全细胞裂解液中 p16 INK4A 表达的 Western 印迹分析.

CDKN2A/p16INK4a 抗体 (JC8): sc-56330

3.5(4)
寫評論提問

说明书
  • CDKN2A/p16 抗体 JC8 是小鼠单克隆 IgG2a, 在299篇文献中引用，提供 200 µg/ml
  • 针对全长重组p16 INK4A，human起源
  • CDKN2A/p16 抗体 (JC8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 p16 INK4A
  • 抗CDKN2A/p16抗体（JC8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CDKN2A/p16 (JC8): sc-56330的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 CDKN2A/p16 抗体 (JC8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CDKN2A/p16 抗体 (JC8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 p16 INK4A/p19 ARF/p14 ARF (CDKN2A) 抗体包括 p14 ARF 和 p19 ARF

p16 INK4A 抗体（JC8）是一种小鼠单克隆 IgG2a 抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人类来源的 p16 INK4A。p16 INK4A（JC8）抗体有非结合型和多种 结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。p16 INK4A通过抑制细胞周期依赖性激酶4（Cdk4）来发挥肿瘤抑制剂的作用，从而防止视网膜母细胞瘤蛋白（Rb）磷酸化并阻止细胞周期从G1期进入S期。这种调节功能可维持正常的细胞周期控制，防止不受控制的细胞增殖，而细胞增殖失控正是癌症的特征。p16 INK4A基因的变异（包括纯合缺失和点突变）在各种人类肿瘤中经常发现，这凸显了其在肿瘤发生中的重要性。使用抗p16 INK4A抗体（JC8）使研究人员能够研究细胞周期调节机制和癌症生物学中的p16 INK4A失调。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CDKN2A/p16 抗体 (JC8)

sc-56330
200 µg/ml
RMB2377.00

CDKN2A/p16INK4a (JC8): m-IgG Fc BP-HRP 套装

sc-525655
200 µg Ab; 10 µg BP
RMB2662.00

CDKN2A/p16INK4a (JC8): m-IgG2a BP-HRP 套装

sc-546354
200 µg Ab; 10 µg BP
RMB2662.00

CDKN2A/p16INK4a 抗体 (JC8) AC

sc-56330 AC
500 µg/ml, 25% agarose
RMB3129.00

CDKN2A/p16INK4a 抗体 (JC8) HRP

sc-56330 HRP
200 µg/ml
RMB2377.00

CDKN2A/p16INK4a 抗体 (JC8) FITC

sc-56330 FITC
200 µg/ml
RMB2482.00

CDKN2A/p16INK4a 抗体 (JC8) PE

sc-56330 PE
200 µg/ml
RMB2580.00

CDKN2A/p16INK4a 抗体 (JC8) Alexa Fluor® 488

sc-56330 AF488
200 µg/ml
RMB2685.00

CDKN2A/p16INK4a 抗体 (JC8) Alexa Fluor® 546

sc-56330 AF546
200 µg/ml
RMB2685.00

CDKN2A/p16INK4a 抗体 (JC8) Alexa Fluor® 594

sc-56330 AF594
200 µg/ml
RMB2685.00

CDKN2A/p16INK4a 抗体 (JC8) Alexa Fluor® 647

sc-56330 AF647
200 µg/ml
RMB2685.00

CDKN2A/p16INK4a 抗体 (JC8) Alexa Fluor® 680

sc-56330 AF680
200 µg/ml
RMB2685.00

CDKN2A/p16INK4a 抗体 (JC8) Alexa Fluor® 790

sc-56330 AF790
200 µg/ml
RMB2685.00

Dopo l'utilizzo di questo anticorpo per un western blot è stato possibile osservare la comparsa di due bande in ogni replicato che ho analizzato. Vorrei sapere a cosa corrispondono le due differenti bande

Asked by: andrea
Grazie per la domanda. Rivolgersi direttamente al servizio tecnico per ricevere assistenza. Thank you for your question. Please contact technical service directly for assistance.
Answered by: Tech Support Europe
Date published: 2024-12-11

Does this antibody apply to mouse or rat tissue for WB or IHC?

Asked by: Matthew
This antibody has only been validated in human tissue
Answered by: Technical Service
Date published: 2023-11-07
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Rated 1 out of 5 by from Non funziona su estratti proteici di topoHo provato la trial size circa un mese fa. Purtroppo non funziona per western blot su estratti proteici preparati da muscolo di topo. Non riconosce la proteina P16 nè nel muscolo di topo WT, nè in di topo trangenico che dovrebbero avere un'induzione di P16. Riconosce solo le catene leggere e pesanti delle immunoglobuline a 20 kDa e 55kDa, rispettivamente
Date published: 2023-04-14
Rated 3 out of 5 by from Weak bands, but can detectThe band was weak but detectable by WB. 1:500 was detectable.
Date published: 2022-11-08
Rated 5 out of 5 by from Western blot excellent detectionHeLa lysate, 40ug total protein load per well, semi-dry transfer, 30 minute block, primary overnight 4 degrees C, secondary goat anti-mouse IgG (H+L) cross-adsorbed HRP 1:10,000 1hr RT, ECL substrate, GeneGnome gel imager photograph. Block and antibody solutions: 5% blotting-grade block in TBST. Excellent detection. May try lower concentration.
Date published: 2020-12-02
Rated 5 out of 5 by from very good signal by WBI used this antibody routinely for the detection of p16 in primary keratinocytes during replicative senescence (1:1000 in 5% milk/PBST ON 4C). MW around 15-20 kDa (using precast or >12% gels). We used primary keratinocytes at passage 4 as positive control of the antibody.
Date published: 2020-04-14
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CDKN2A/p16INK4a 抗体 (JC8) is rated 3.5 out of 5 by 4.
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