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p-VASP Antibody (16C2) - Western Blotting - Image 72649
未经处理 (A, C) 和经λ蛋白磷酸酶处理 (B, D) 的人血小板提取物中 VASP 磷酸化的 Western 印迹分析. 测试的抗体包括 p-VASP 抗体 (16C2): sc-101439 (A, B) 和 VASP (A-11): sc-46668 (C, D).
未经处理 (A, C) 和经λ蛋白磷酸酶处理 (B, D) 的人血小板提取物中 VASP 磷酸化的 Western 印迹分析. 测试的抗体包括 p-VASP 抗体 (16C2): sc-101439 (A, B) 和 VASP (A-11): sc-46668 (C, D).

p-VASP 抗体 (16C2): sc-101439

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说明书
  • p-VASP 抗体 16C2 是小鼠单克隆 IgG1, 在10篇文献中引用，提供 50 µg/0.5 ml
  • 针对human来源的合成VASP，在丝氨酸239处磷酸化
  • 推荐用于 mouse, rat 和 human 来源的Ser 239 phosphorylated VASP WB, IP, IF, FCM 和 ELISA检测
  • 目前，我们还没有完成p-VASP 抗体 (16C2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Wiskott-Aldrich综合征（WAS）以血小板减少、湿疹、细胞介导和体液免疫缺陷以及对淋巴增生性疾病的易感性为特征。该综合征是由编码富含脯氨酸的蛋白质（称为WASP）的基因突变引起的。WASP已被鉴定为Cdc42的下游效应子，并已涉及肌动蛋白聚合和细胞骨架组织。一个远缘相关蛋白，VASP（血管扩张刺激磷蛋白），通过与类肌动蛋白丝相互作用参与细胞结构的维持。VASP与WASP的氨基末端具有有限的同源性，后者在WAS患者中经常发生突变。作为cAMP和cGMP依赖性激酶的既定底物，VASP在调节性丝氨酸残基157上被磷酸化，并定位于粘着斑、微丝和质膜的高活性区域。VASP在人血小板中高表达，与WASP一样，可能在细胞骨架组织中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-VASP 抗体 (16C2) 参考文献:

  1. 中性粒细胞衍生的谷氨酸可调节血管内皮屏障功能。  |  Collard, CD., et al. 2002. J Biol Chem. 277: 14801-11. PMID: 11847215
  2. VASP 与 TRPC4 在 PKG 介导的系膜细胞储存操作钙反应抑制中的关联。  |  Wang, X., et al. 2007. Am J Physiol Renal Physiol. 293: F1768-76. PMID: 17913834
  3. 分析前样品制备导致的血小板内磷酸-VASP 表达变化，说明血小板药理学中的质量控制问题。  |  Gharehbaghian, A., et al. 2015. Iran J Pharm Res. 14: 321-8. PMID: 25561938
  4. PDE3A 基因突变导致常染色体显性高血压并伴有手足畸形。  |  Maass, PG., et al. 2015. Nat Genet. 47: 647-53. PMID: 25961942
  5. 血管扩张剂刺激的磷蛋白活性是烧伤柯西氏杆菌在人类巨噬细胞中生长的必要条件  |  Colonne, PM., et al. 2016. PLoS Pathog. 12: e1005915. PMID: 27711191
  6. 血管扩张剂刺激的磷蛋白（VASP）不是大鼠血小板聚集、血栓形成、止血和普拉格雷抗血小板作用的主要介质。  |  Ito, Y., et al. 2018. Sci Rep. 8: 9955. PMID: 29967338
  7. 子痫前期 cGMP 依赖性蛋白激酶 I 介导的蜕膜化异常。  |  Chen, J., et al. 2021. Hypertens Res. 44: 318-324. PMID: 33093639
  8. MAP4K4 抑制剂在血小板聚集和血小板介导的血块回缩过程中的新功能。  |  Nam, GS., et al. 2021. Biochem Pharmacol. 188: 114519. PMID: 33737052
  9. 尼可刹米治疗糖尿病大鼠胸主动脉的内皮依赖性松弛得到改善  |  Engin, S., et al. 2021. Cardiovasc Toxicol. 21: 563-571. PMID: 33772737
  10. 青蒿琥酯通过调节 cAMP 和 PI3K/MAPK 通路对人体血小板的抗血栓作用  |  Yoon, SS., et al. 2022. Int J Mol Sci. 23: PMID: 35163507

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产品名称产品编号规格价格数量收藏夹

p-VASP 抗体 (16C2)

sc-101439
50 µg/0.5 ml
RMB2377.00

I intend to use this p-VASP antibody for FCM assay. What would be an appropriate secondary antibody to use?

Asked by: joegzhou
Thank you for your question.At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-VASP Antibody (16C2). This work is in progress.
Answered by: BlakeJ
Date published: 2024-08-20

What is the recommended starting dilution for Western blot for p-VASP (16C2): sc-101439 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-101439.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-04-05
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Rated 5 out of 5 by from We have used this antibody in WB and it workedWe have used this antibody in WB and it worked really well for us.
Date published: 2015-06-15
Rated 5 out of 5 by from Published western blot data using this antibodyPublished western blot data using this antibody with rat thoracic aorta samples. -SCBT Publication Review
Date published: 2015-02-06
Rated 5 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-01-05
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p-VASP 抗体 (16C2) is rated 5.0 out of 5 by 3.
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