Date published: 2025-9-6

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p-Stat4 抗体 (E-2): sc-28296

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说明书
  • p-Stat4抗体(E-2)是小鼠单克隆IgG1 κ, 在17篇文献中引用,规格为200 µg/ml
  • 针对含有磷酸化丝氨酸721的短氨基酸序列
  • p-Stat4 抗体 (E-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ,检测 mouse, rat, human 和 来源的Ser 721 phosphorylated Stat4; 也和以下物种反应,包括: 和 equine, canine, bovine, porcine and avian
  • 抗p-Stat4抗体(E-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-28296 X, 200 µg/0.1 ml)
  • 关于如何获取p-Stat4 (E-2): sc-28296免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 p-Stat4 抗体 (E-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-Stat4 抗体 (E-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p-Stat4抗体(E-2)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附 检测(ELISA)。p-Stat4 (E-2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合物。Stat4在免疫反应中起着至关重要的作用,特别是介导白介素-12(IL-12)信号,而IL-12对于T辅助细胞1(Th1)的分化至关重要。在IL-12的激活下,Stat4发生磷酸化,导致二聚化并随后转移到细胞核,Stat4与特定的DNA元件结合以调节基因表达。Ser 721的磷酸化作用尤为重要,因为这种修饰是Stat4转录活性所必需的,并且依赖于p38 MAPK信号传导,突出了控制免疫反应的复杂调节机制。抗Stat4抗体(E-2)可特异性检测这种磷酸化形式,使得p-Stat4(E-2)单克隆抗体成为研究人员研究免疫信号传导通路和Stat4在各种生物过程中的作用的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Stat4 抗体 (E-2) 参考文献:

  1. STAT5的细胞膜酪氨酸去磷酸化。SHP-2 在 STAT5 调节中的潜在作用。  |  Yu, CL., et al. 2000. J Biol Chem. 275: 599-604. PMID: 10617656
  2. IL-12 通过磷脂酰肌醇 3- 激酶和 Ras 依赖性信号转导途径选择性地调节 STAT4。  |  Athié-M, V., et al. 2000. Eur J Immunol. 30: 1425-34. PMID: 10820390
  3. STAT 的丝氨酸磷酸化。  |  Decker, T. and Kovarik, P. 2000. Oncogene. 19: 2628-37. PMID: 10851062
  4. MKK6/p38 通路对白细胞介素-12 诱导的 STAT4 丝氨酸磷酸化和转录活性的重要性  |  Visconti, R., et al. 2000. Blood. 96: 1844-52. PMID: 10961885
  5. 通过流式细胞仪检测细胞内磷酸化的 STAT-4。  |  Uzel, G., et al. 2001. Clin Immunol. 100: 270-6. PMID: 11513540
  6. 用 HTLV-I 转化的 T 细胞中 STAT4 的酪氨酸和丝氨酸显性磷酸化。  |  Higashi, T., et al. 2005. Genes Cells. 10: 1153-62. PMID: 16324152
  7. 牛皮癣保护性 TYK2 I684S 变体会损害皮肤归巢 CD4+ 和 CD8+ 记忆 T 细胞的 IL-12 刺激 pSTAT4 反应。  |  Enerbäck, C., et al. 2018. Sci Rep. 8: 7043. PMID: 29728633
  8. STAT4 T辅助细胞磷酸化可预测难治性慢性鼻-鼻窦炎的外科手术结果。  |  Abidin, MR., et al. 2023. medRxiv.. PMID: 38168423
  9. 唾液腺上皮细胞中下调的 GPX4 通过脂质 ROS/pSTAT4/AQP5 轴导致了 Sjogren 综合征患者唾液分泌功能障碍。  |  Zhou, J., et al. 2024. Free Radic Biol Med. 218: 1-15. PMID: 38574973
  10. 对多肽配体的转录反应:JAK-STAT 途径。  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
  11. Stat4,一种在早期骨髓分化过程中表达的新型γ干扰素激活位点结合蛋白。  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
  12. Jak-STAT 通路和转录激活对 IFNs 及其他细胞外信号蛋白的反应。  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

订购信息

产品名称产品编号规格价格数量收藏夹

p-Stat4 抗体 (E-2)

sc-28296
200 µg/ml
RMB2377.00

p-Stat4 (E-2): m-IgG Fc BP-HRP 套装

sc-528411
200 µg Ab; 10 µg BP
RMB2662.00

p-Stat4 (E-2): m-IgGκ BP-HRP 套装

sc-520785
200 µg Ab, 40 µg BP
RMB2662.00

p-Stat4 (E-2): m-IgG1 BP-HRP 套装

sc-542919
200 µg Ab; 20 µg BP
RMB2662.00

p-Stat4 抗体 (E-2) X

sc-28296 X
200 µg/0.1 ml
RMB2377.00

p-Stat4 抗体 (E-2) AC

sc-28296 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Stat4 抗体 (E-2) HRP

sc-28296 HRP
200 µg/ml
RMB2377.00

p-Stat4 抗体 (E-2) FITC

sc-28296 FITC
200 µg/ml
RMB2482.00

p-Stat4 抗体 (E-2) PE

sc-28296 PE
200 µg/ml
RMB2580.00

p-Stat4 抗体 (E-2) Alexa Fluor® 488

sc-28296 AF488
200 µg/ml
RMB2685.00

p-Stat4 抗体 (E-2) Alexa Fluor® 546

sc-28296 AF546
200 µg/ml
RMB2685.00

p-Stat4 抗体 (E-2) Alexa Fluor® 594

sc-28296 AF594
200 µg/ml
RMB2685.00

p-Stat4 抗体 (E-2) Alexa Fluor® 647

sc-28296 AF647
200 µg/ml
RMB2685.00

p-Stat4 抗体 (E-2) Alexa Fluor® 680

sc-28296 AF680
200 µg/ml
RMB2685.00

p-Stat4 抗体 (E-2) Alexa Fluor® 790

sc-28296 AF790
200 µg/ml
RMB2685.00

p-Stat4 (E-2) 中和勝肽

sc-28296 P
100 µg/0.5 ml
RMB511.00

Can p-Stat4 (E-2): sc-28296 be frozen?

Asked by: jenniferc
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 15
Date published: 2017-06-07
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Rated 3 out of 5 by from goodIt has a perfect bind but the bind is not what I want .
Date published: 2017-10-05
Rated 5 out of 5 by from Good picturei got this 2 weeks ago, picture is clear, very helpful.
Date published: 2017-09-08
Rated 5 out of 5 by from p-Stat4 (E-2):sc-28296 抗体使用对象我在前段时间申请的p-Stat4单克隆抗体使用装,我的研究对象为草鱼,先前看到这个抗体的使用面为人,鼠,牛,猪和鸟类,通过比较磷酸化位点的保守性,我们发现这个抗体应该可以使用。后来发现效果比较好
Date published: 2017-06-23
Rated 5 out of 5 by from Very potent and specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of ~70 kDa STAT4 in overnight serum-starved human A431 cells treated for 5 minutes with EGF on Western blots, and no significant cross-reactivities on these blots with any off-target proteins, except for a prominent 160 kDa protein.
Date published: 2017-03-14
Rated 5 out of 5 by from Specific for Ser 721This mono is specific for Ser 721 phosphorylated Stat4 and suitable for detection in a broad range of species including mouse, rat, human, equine, canine, bovine, porcine and avian.
Date published: 2016-12-29
Rated 5 out of 5 by from Works great for WB in human and mouse CD4+ T cellsWorks great for WB in human and mouse CD4+ T cells. -SCBT Publication Review
Date published: 2015-03-26
Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody, works well to detect phosphorylation in untreated and lambda protein phosphatase (sc-200312A) treated HEK293 whole cell lysates. -SCBT QC
Date published: 2015-03-12
Rated 4 out of 5 by from WB: Antibody is reasonably specific and sensitiveWB: Antibody is reasonably specific and sensitive in detecting phosphorylation in untreated and PMA + LPS-treated RAW 264.7 whole cell lysates. -SCBT QC
Date published: 2015-02-04
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p-Stat4 抗体 (E-2) is rated 4.7 out of 5 by 10.
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