Date published: 2025-9-6
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p-Stat3 Antibody (pS727.25) - Western Blotting - Image 400936
p-Stat3 抗体 (pS727. 25): sc-293059. 未转染 293T: sc-117752 (A), 转染人 Stat3 的 293T: sc-177985 (B), 未处理的 A-431 (C) 和 EGF 处理的 A-431 (D) 全细胞裂解液中 p-Stat3 表达的 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.
p-Stat3 Antibody (pS727.25) - Western Blotting - Image 400936
p-Stat3 抗体 (pS727. 25): sc-293059. 未转染 293T: sc-117752 (A), 转染人 Stat3 的 293T: sc-177985 (B), 未处理的 A-431 (C) 和 EGF 处理的 A-431 (D) 全细胞裂解液中 p-Stat3 表达的 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.
p-Stat3 抗体 (pS727. 25): sc-293059. 未转染 293T: sc-117752 (A), 转染人 Stat3 的 293T: sc-177985 (B), 未处理的 A-431 (C) 和 EGF 处理的 A-431 (D) 全细胞裂解液中 p-Stat3 表达的 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.

p-Stat3 抗体 (pS727.25): sc-293059

3.7(16)
寫評論提問

说明书
  • p-Stat3抗体(pS727.25)是小鼠单克隆IgG1 κ, 在10篇文献中引用，规格为200 µg/ml
  • 针对含Stat3的human起源的磷酸化Ser 727的短氨基酸序列
  • 推荐用于 human 和 rat 来源的Ser 727 phosphorylated Stat3 of human and rat origin WB, IP 和 IF检测; 不会与 Ser 727 phosphorylated Stat3 of mouse origin产生交联反应
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-293059 X, 200 µg/0.1 ml)
  • 1 BP-HRP">m-IgG1 BP-HRP是p-Stat3 Antibody (pS727.25) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与p-Stat3 Antibody (pS727.25) 打包提供（请参阅下面的订购信息）。

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関連項目

附加Stat抗体，包括Stat1, Stat2, Stat3, Stat4, Stat5和Stat6

各种配体（包括干扰素和EGF等生长激素）的膜受体信号传导可诱导JAK激酶的活化，从而导致各种Stat转录因子的酪氨酸磷酸化。Stat1和Stat2受IFN-α诱导形成异源二聚体，是ISGF3转录因子复合物的一部分。虽然早期报道表明Stat3受EGF和IL-6激活，但已经表明Stat3β似乎受两者激活，而Stat3α则受EGF激活，但不受IL-6激活。Stat4在睾丸和髓系细胞中表达最高。已鉴定IL-12为Stat4的激活剂。已表明Stat5可被催乳素和IL-3激活。Stat6参与IL-4激活的信号通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p-Stat3 抗体 (pS727.25)

sc-293059
200 µg/ml
RMB2377.00

p-Stat3 (pS727.25): m-IgG1 BP-HRP 套装

sc-550932
200 µg Ab; 20 µg BP
RMB2662.00

p-Stat3 抗体 (pS727.25) X

sc-293059 X
200 µg/0.1 ml
RMB2377.00

Can this antibody be used to perform immunofluorescence on paraffin embedded tissue sections ?

Asked by: DH55
Thank you for your question. This antibody is recommended for immunocytochemistry, but not for immunohistochemistry. We do not recommend it for paraffin-embedded tissue samples.
Answered by: Tech Support Europe
Date published: 2021-02-03

What is the total volume provided of p-Stat3 (pS727.25): sc-293059 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. p-Stat3 (pS727.25): sc-293059 is provided as 200 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2019-06-19
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Rated 1 out of 5 by from Nos nos ha funcionado.Usamos una muestra de este anticuerpo y no nos ha dado buena detección.
Date published: 2019-12-14
Rated 3 out of 5 by from Weak bands for WBGave weak bands when using at 1:200 for mouse samples. May work for others samples though.
Date published: 2018-03-22
Rated 5 out of 5 by from GreatThese are trial samples, including p-Stat3 and Stat3, but help me complete one set of experimental.
Date published: 2018-02-14
Rated 3 out of 5 by from only work in A549 cell line.p-STAT3 can only be detected in A549 cell line not in breast cancer cell lines
Date published: 2017-10-12
Rated 3 out of 5 by from weakmay be it is not suitable for my cell, because I can't get a good bind in western blot
Date published: 2017-10-05
Rated 3 out of 5 by from works with weak signalThis antibody doesn't give a very strong positive band.Bands are not clear.
Date published: 2017-09-28
Rated 4 out of 5 by from GoodThe WB result was specific,clear and strong.I will support your products!
Date published: 2017-09-15
Rated 5 out of 5 by from goodThis is antibody is specific great antibody for Western blotting
Date published: 2017-09-07
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p-Stat3 抗体 (pS727.25) is rated 3.7 out of 5 by 16.
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