Date published: 2025-9-11

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p-Ser/Phosphoserine 抗体 (4A3): sc-81516

3.9(10)
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说明书
  • p-Ser/Phosphoserine抗体(4A3)是小鼠单克隆IgM κ, 在10篇文献中引用,规格为50 µg/0.5 ml
  • 针对磷丝氨酸
  • 推荐用于 mouse, rat, human 和 canine 来源的a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine WB 和 IP检测
  • 关于如何获取p-Ser/Phosphoserine (4A3): sc-81516免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成p-Ser/Phosphoserine 抗体 (4A3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

p-Ser/磷酸丝氨酸抗体(4A3)是一种IgM κ小鼠单克隆p-Ser抗体(也称为磷酸化丝氨酸抗体),它可通过蛋白质印迹和免疫沉淀检测粗细胞提取物中范围广泛的丝氨酸磷酸化蛋白质,更偏好于直接邻近小鼠、大鼠和人源磷酸丝氨酸的正电荷氨基酸。p-Ser/磷酸丝氨酸抗体(4A3)为非偶联抗p-Ser抗体形式。蛋白激酶催化目标底物中丝氨酸、苏氨酸或酪氨酸残基的磷酸化,为无数细胞信号通路提供了一种控制机制。几个激酶家族在目标底物中磷酸化丝氨酸和苏氨酸残基,包括Raf、Rsk、ROCK、PAK、Ak和PKC家族的丝氨酸/苏氨酸蛋白激酶。蛋白质通过磷酸化修饰可导致其结构的三维变化,从而改变其酶活性或与其他蛋白质相互作用的能力。针对磷酸化丝氨酸的抗体可用于表征具有磷酸化丝氨酸残基的蛋白质,并阐明涉及丝氨酸磷酸化的细胞通路。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

p-Ser/Phosphoserine 抗体 (4A3)

sc-81516
50 µg/0.5 ml
RMB2377.00

What is the recommended storage condition for p-Ser (4A3): sc-81516?

Asked by: jerojero
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2018-06-03
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Rated 5 out of 5 by from Works Great!Used this antibody to look at pSer of my protein after 6-Bnz-cAMP PKA activator treatment. Used at 1:500 in 1% milk-PBS(T) for 2h at RT. I think 1:1000 should also work fine.
Date published: 2022-01-26
Rated 3 out of 5 by from worked in WBPhosphorylation was detected in WB using human lysate derived pull-downed protein.
Date published: 2019-07-23
Rated 1 out of 5 by from Not working for WB in our handWe used his antibody for Western blot using human cell nuclear extract. Surprising to us, we did not detect any signal considering so many good reviews from other users.
Date published: 2018-06-05
Rated 5 out of 5 by from Work well in WB.I got this sample, work well in WB after IP. Possibly need optimization in case.
Date published: 2018-03-13
Rated 3 out of 5 by from Better than 16B4Tested this on Hela lysates. The signals detected were stronger than the 16B4 pSer ab. Background was fine.
Date published: 2018-02-14
Rated 4 out of 5 by from Works for IP samplesI used the antibody for post-IP Western blot. I diluted the antibody 1:200 in milk overnight. Saw clear bands. I think 1:500 dilution should also work well.
Date published: 2017-12-01
Rated 4 out of 5 by from Nice antibodyWe used it last month for IP. After IP with this antibody we probed with many other antibodies. Works well.
Date published: 2017-11-13
Rated 5 out of 5 by from has the high stablityWorks well for Western blot at 1:300, this antibody is good.
Date published: 2017-08-15
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p-Ser/Phosphoserine 抗体 (4A3) is rated 3.9 out of 5 by 10.
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