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p-Ser/Phosphoserine 抗体 (16B4): sc-81514

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说明书
  • p-Ser/Phosphoserine 抗体 16B4 是小鼠单克隆 IgM κ，p-Ser/Phosphoserine抗体, 在95篇文献中引用，规格为50 µg/0.5 ml
  • 针对磷丝氨酸
  • 推荐用于 mouse, rat, human 和 canine 来源的a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine WB 和 IP检测
  • 关于如何获取p-Ser/Phosphoserine (16B4): sc-81514的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成p-Ser/Phosphoserine 抗体 (16B4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

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此外 p-Ser 抗体包括 p-Thr 和 p-Tyr

p-Ser抗体（16B4）是一种IgM κ小鼠单克隆p-Ser抗体（也称为磷酸化丝氨酸抗体、磷酸丝氨酸抗体或p-丝氨酸抗体），可通过蛋白质印迹和免疫沉淀检测小鼠、大鼠、人和犬源的p-Ser蛋白。p-Ser抗体（16B4）为非偶联抗p-Ser抗体。蛋白激酶催化目标底物中丝氨酸、苏氨酸或酪氨酸残基的磷酸化，为无数细胞信号通路提供了一种控制机制。几个激酶家族在目标底物中磷酸化丝氨酸和苏氨酸残基，包括Raf、Rsk、ROCK、PAK、Ak和PKC家族的丝氨酸/苏氨酸蛋白激酶。蛋白质通过磷酸化修饰可导致其结构的三维变化，从而改变其酶活性或与其他蛋白质相互作用的能力。针对磷酸化丝氨酸的抗体可用于表征具有磷酸化丝氨酸残基的蛋白质，并阐明涉及丝氨酸磷酸化的细胞通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Ser/Phosphoserine 抗体 (16B4) 参考文献:

  1. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  2. 从微生物到高等真核生物，磷丝氨酸氨基转移酶具有保守的活性位点，但存在细微差异。  |  Singh, RK., et al. 2021. Protein Pept Lett. 28: 996-1008. PMID: 33588715
  3. 磷丝氨酸磷酸酶的下调可增强肿瘤免疫环境，从而提高免疫检查点阻断疗法的效果。  |  Peng, ZP., et al. 2023. J Immunother Cancer. 11: PMID: 36849198
  4. 磷丝氨酸负载的壳聚糖膜通过激活内源性干细胞促进骨再生。  |  Ke, Y., et al. 2023. Front Bioeng Biotechnol. 11: 1096532. PMID: 37034248
  5. 大肠杆菌中不可水解的磷丝氨酸在重组蛋白中的生物合成和遗传编码。  |  Zhu, P., et al. 2023. Bio Protoc. 13: e4861. PMID: 37969748
  6. 过量磷丝氨酸-129 α-突触核蛋白诱导脊椎动物突触囊泡运输和去簇缺陷。  |  Wallace, JN., et al. 2024. Mol Biol Cell. 35: ar10. PMID: 37991902
  7. 由 MEK 和 MKK 同工酶定义的独立人类 MAP 激酶信号转导途径。  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
  8. 哺乳动物 MAP 激酶激酶（MEK1）的组成突变体和假定调节丝氨酸磷酸化位点。  |  Pagès, G., et al. 1994. EMBO J. 13: 3003-10. PMID: 8039496
  9. 丝裂原活化蛋白激酶和 90-kDa 核糖体 S6 激酶对 c-Fos 转抑结构域的磷酸化。  |  Chen, RH., et al. 1993. Proc Natl Acad Sci U S A. 90: 10952-6. PMID: 8248197
  10. ROCK-I和ROCK-II，小鼠Rho相关线圈形成蛋白丝氨酸/苏氨酸激酶的两种异构体。  |  Nakagawa, O., et al. 1996. FEBS Lett. 392: 189-93. PMID: 8772201
  11. 人类 Ste20 同源物 hPAK1 将 GTPases 与 JNK MAP 激酶通路连接起来。  |  Brown, JL., et al. 1996. Curr Biol. 6: 598-605. PMID: 8805275
  12. 新型磷丝氨酸磷酸酶抑制剂。  |  Hawkinson, JE., et al. 1997. Eur J Pharmacol. 337: 315-24. PMID: 9430431

订购信息

产品名称产品编号规格价格数量收藏夹

p-Ser/Phosphoserine 抗体 (16B4)

sc-81514
50 µg/0.5 ml
RMB2377.00

Is there any possibility that p-Ser Antibody (16B4): sc-81514 does work for yeast samples as well...?

Asked by: yolyol
Thank you for your question. This antibody has only been tested in samples of mouse, rat, human and canine origin. It has not been tested in yeast species.
Answered by: Tech Support Europe
Date published: 2021-02-26

Hello, Which buffer is better to detect p-Ser antibody, skim-milk or 3-5% BSA? I immunoprecipitated phospho-protein and used 3% BSA in TBS-T buffer for blocking and 1st antibody but I couldn't get specific phosphorylation band.

Asked by: chohee0720
Thank you for your question. Please contact Technical Service directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2022-07-16

HI , I recently purchased this pserine antibody and I do see a band at 20kDa. why do i see a band arounf 20kda for pSerine?

Asked by: Dhanusha
Thank you for your question. As p-Ser Antibody (16B4): sc-81514 detects a broad range of serine-phosphorylated proteins in crude cell extracts, a signal at 20 kDa can respond to a large variety of different proteins of this molecular weight. The identity of this protein could be determined by assays such as MALDI-TOF.
Answered by: Tech Support Europe
Date published: 2020-07-14

The p-Ser Antibody (16B4) antibody is a monoclonal mouse IgM κ (kappa light chain). Still, you recommend the m-IgGκ BP-HRP: sc-516102 as a secondary antibody. But this a IgG-kappa specific ab. Would it still work?

Asked by: PeterPM
Thank you for your question. Yes, m-IgGκ BP-HRP: sc-516102 can detect any antibody that possesses a kappa light chain, including p-Ser Antibody (16B4): sc-81514, other IgM, and IgA antibodies.
Answered by: Technical Support Europe
Date published: 2019-05-06

Just wondering if your products of sc-81516 and sc-81514 recognize any of the phosphorylated S/T/Y, or they require certain residues beside these phosphorylated residues (like a certain motif).

Asked by: Zhyuhao
Thank you for your question. These antibodies both detect a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine. Additional information regarding the detected residues is not available.
Answered by: Tech Service
Date published: 2019-04-10

"preferring positively charged amino acids directly neighbored to phosphoserine". Does it mean that phosphoserine without neighboring positively charged amino acids will not be recognized by the 16B4 Ab ?

Asked by: Yo14
Thank you for your question. The antibody recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with pSK and pSP motifs strongly, recognizing substrates of MAP/SAP kinases and CDC kinases. Phosphoserine detection using an antibody generally is affected by the surrounding amino acids sequence, but it is possible that it can detect phosphoserine without neighboring + charged amino acids.
Answered by: Tech Service
Date published: 2018-08-09

Hi, is there any possibility to receive a sample from this antibody? Thanks for your answer.

Asked by: Constanze
Thank you for your question. We do not currently offer sample sizes for our p-Ser Antibody (16B4): sc-81514. This is a very popular and well cited antibody. If you purchase the antibody, please know that it is covered by our generous product warranty for one year from the date of purchase. If it does not work in any of the recommended applications, please contact our Technical Service Department and we can provide you with a replacement or refund.
Answered by: Technical Support
Date published: 2017-03-01

What is the expected molecular weight in Western blot when using p-Ser Antibody (16B4): sc-81514?

Asked by: cjMara
Thank you for your question. This antibody is recommended for detection of a broad range of serine-phosphorylated proteins. Therefore, the observed molecular weight is dependent upon which specific phosphorylated protein(s) you are evaluating with this antibody.
Answered by: Technical Support
Date published: 2016-12-22
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Rated 4 out of 5 by from p-Ser (16B4): sc-81514Western blot: Detecta multiples proteínas en extractos totales de células CRFK con 20 ug de proteína a una dilución de 1:500 y 1:1000 IP: Se utilizó 5 y 7 uL para inmunoprecipitar proteínas fosforiladas de células CRFK. Se utilizó la dilución de 1:500 para identificar las proteínas inmunoprecipitadas y sí se observan diversas bandas con diferentes pesos moleculares
Date published: 2024-04-01
Rated 5 out of 5 by from Works greatIt adequately detects phosphoserine antibodies. Even though it has some background, you can clean it up by more TBS T20 washes.
Date published: 2024-03-28
Rated 1 out of 5 by from 使えなかった一切バンドがでなかった。通常リン酸化抗体はブロードなバンドがみえるが、ひとつもバンドがみえなかった。
Date published: 2020-11-18
Rated 3 out of 5 by from Pretty good antibodyThe antibody works pretty well upon immunoprecipitation of the phosphorylated protein. On total cellular extract is too weak.
Date published: 2020-10-07
Rated 3 out of 5 by from Worked in WBphosphorylation was detected in Pull downed protein in WB
Date published: 2019-07-23
Rated 5 out of 5 by from Successful detectionSuccessful detection of Ser phosphorylation in immunoprecipitates
Date published: 2018-12-07
Rated 3 out of 5 by from P-Ser AntibodyCompared to other p-ser abs there was a lower background. Signal was much lower too. Tested in HeLa total lysates.
Date published: 2018-02-14
Rated 3 out of 5 by from Nice antibodyWe used this antibody for IP last month. It works well for some primary proteins. Overall a decent buy.
Date published: 2017-11-13
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p-Ser/Phosphoserine 抗体 (16B4) is rated 4.0 out of 5 by 24.
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