Date published: 2025-9-7
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p-RKIP Antibody (80.Ser 153) - Western Blotting - Image 411224
p-RKIP Antibody (80.Ser 153): sc-135779. Western blot analysis of RKIP phosphorylation in HeLa whole cell lysate. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.
p-RKIP Antibody (80.Ser 153): sc-135779. Western blot analysis of RKIP phosphorylation in HeLa whole cell lysate. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.

p-RKIP 抗体 (80.Ser 153): sc-135779

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说明书
  • p-RKIP抗体(80.Ser 153)是小鼠单克隆IgG2a κ, 在2篇文献中引用，规格为200 µg/ml
  • 针对含磷酸化丝氨酸153的短氨基酸序列的抗体，RKIP human来源
  • 推荐用于 human 来源的Ser 153 phosphorylated RKIP WB, IP 和 ELISA检测
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 关于如何获取p-RKIP (80.Ser 153): sc-135779的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是p-RKIP Antibody (80.Ser 153) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p-RKIP Antibody (80.Ser 153) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p-RKIP (80.Ser 153) 是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过 Western 印迹（WB）和酶联免疫吸附试验（ELISA）检测 Ser 153 磷酸化的 RKIP。Raf 激酶抑制蛋白（RKIP）又称 PEBP，在调节 Raf/MAPK 信号通路方面起着至关重要的作用，而 Raf/MAPK 信号通路对细胞生长、分化和存活的调节至关重要。RKIP 丝氨酸 153 处的磷酸化会影响 RKIP 与信号级联中其他蛋白质相互作用的能力。当蛋白激酶 C 在植物醇酯或表皮生长因子等刺激下磷酸化 RKIP 时，RKIP 会发生构象变化，从而与 Raf-1 分离，并与 G 蛋白偶联受体激酶 2（GRK2）结合。这种相互作用能有效抑制 GRK2 的活性，从而影响下游信号事件。RKIP 调节这些通路的能力对肿瘤抑制功能至关重要，它能抑制 NFkB 对 TNF-α 和白细胞介素-1 beta 等炎症细胞因子的激活，最终影响癌症的进展和转移。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-RKIP 抗体 (80.Ser 153) 参考文献:

  1. RKIP 对 Raf-1 激酶活性和 MAP 激酶信号的抑制。  |  Yeung, K., et al. 1999. Nature. 401: 173-7. PMID: 10490027
  2. raf激酶抑制蛋白抑制Raf/MEK/细胞外信号调节激酶途径的机制。  |  Yeung, K., et al. 2000. Mol Cell Biol. 20: 3079-85. PMID: 10757792
  3. 来自大肠杆菌的 YBHB 和 YBCL 的晶体结构，它们是 Raf 激酶抑制蛋白的两种细菌同源物。  |  Serre, L., et al. 2001. J Mol Biol. 310: 617-34. PMID: 11439028
  4. Raf 激酶抑制蛋白与 NF-kappaB 诱导激酶和 TAK1 相互作用，抑制 NF-kappaB 的激活。  |  Yeung, KC., et al. 2001. Mol Cell Biol. 21: 7207-17. PMID: 11585904
  5. 蛋白激酶 C 通过涉及 Raf 激酶抑制蛋白的机制激活 Raf-1 信号。  |  Corbit, KC., et al. 2003. J Biol Chem. 278: 13061-8. PMID: 12551925
  6. 蛋白激酶 C 将 Raf 激酶抑制剂从 Raf-1 转换到 GRK-2。  |  Lorenz, K., et al. 2003. Nature. 426: 574-9. PMID: 14654844
  7. 利妥昔单抗抑制 Raf-MEK1/2-ERK1/2 信号通路, 下调 Bcl-xL 以及非霍奇金淋巴瘤 B 细胞的化疗敏感性。  |  Jazirehi, AR., et al. 2004. Cancer Res. 64: 7117-26. PMID: 15466208
  8. 前列腺癌转移抑制蛋白的生物学特性：Raf 激酶抑制蛋白  |  Keller, ET., et al. 2005. J Cell Biochem. 94: 273-8. PMID: 15565643
  9. Raf 激酶抑制蛋白能调节 Raf-1 而非 B-Raf 激酶的活化。  |  Trakul, N., et al. 2005. J Biol Chem. 280: 24931-40. PMID: 15886202
  10. 磷酸化Raf激酶抑制蛋白（pRKIP）的表达是肺癌存活的预测指标。  |  Huerta-Yepez, S., et al. 2011. BMC Cancer. 11: 259. PMID: 21689459
  11. CDK5介导的RKIP磷酸化与自噬调节帕金森氏症中的神经元死亡。  |  Wen, Z., et al. 2014. Neurobiol Aging. 35: 2870-2880. PMID: 25104559
  12. 蛋白激酶 C 介导的 RKIP 磷酸化调节白三烯 D（4）对肠上皮细胞中 Na - 丙氨酸共转运的抑制。  |  Arthur, S. and Sundaram, U. 2014. Am J Physiol Cell Physiol. 307: C1010-6. PMID: 25231108

订购信息

产品名称产品编号规格价格数量收藏夹

p-RKIP 抗体 (80.Ser 153)

sc-135779
200 µg/ml
RMB2377.00

p-RKIP (80.Ser 153): m-IgG Fc BP-HRP 套装

sc-528792
200 µg Ab; 10 µg BP
RMB2662.00

p-RKIP (80.Ser 153): m-IgGκ BP-HRP 套装

sc-521301
200 µg Ab, 40 µg BP
RMB2662.00

p-RKIP (80.Ser 153): m-IgG2a BP-HRP 套装

sc-547172
200 µg Ab; 10 µg BP
RMB2662.00

p-RKIP 抗体 (80.Ser 153) AC

sc-135779 AC
500 µg/ml, 25% agarose
RMB3129.00

p-RKIP 抗体 (80.Ser 153) HRP

sc-135779 HRP
200 µg/ml
RMB2377.00

Gent.mi,  How did you check the specificity of this antibody?  We are testing it by ELISA but It seems that It recognizes both native and p-153 RKIP with the same affinity...It should not happen. 

Asked by: Marrabio10
Thank you for your question. p-RKIP Antibody (80.Ser 153): sc-135779 has been validated by WB and ELISA for specificity against Ser 153 phosphorylated RKIP of human origin. Please contact our Technical Service Department for help with troubleshooting the antibody if you have results that indicate the antibody is non-specifically recognizing the non-phosphorylated antigen. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-24

How much sample do you recommend loading on the gel for Western blot detection using p-RKIP (80.Ser 153): sc-135779?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Good antibody but have a week band at liver sampleGood antibody but have a week band at normal liver sample
Date published: 2017-12-04
Rated 5 out of 5 by from Works well for Western blottingHave used this antibody on Western blots and it works very well. Would recommend for Westerns.
Date published: 2017-07-21
Rated 5 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is recommended for Western blotting. There was very strong detection of RKIP in 30 minute anisomycin-treated human HeLa cells on Western blots, and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2017-03-14
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p-RKIP 抗体 (80.Ser 153) is rated 4.0 out of 5 by 4.
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